High expression of INHBA is an adverse prognostic factor for<i>de novo</i>acute myeloid leukemia

Si, Ting; Lu, Yunlong; Li, Fenglin; Jiang, Lei; Pei, Renzhi; Zhou, Jeff X.

doi:10.1080/10428194.2017.1324157

Cited by 13 publications

(14 citation statements)

References 35 publications

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“…Activin A was reported to cause apoptosis of myeloma cells and chronic myeloid leukemia cells (10,11). On the other hand, similar to our results, high expression of βA subunit, which composes activin A, is an adverse prognostic factor for acute myeloid leukemia (12). Considering that activin A can affect tumor progression in an indirect manner, such as inhibiting anti-tumor immunity or attracting macrophages that helps tumor development (4,13), our results and the previous report suggest that activin A may be associated with progression of some other hematological malignancies in addition to MF/SS.…”

Section: Discussionsupporting

confidence: 90%

Increased Serum Activin A Levels in Mycosis Fungoides and Sézary Syndrome

Oka

Miyagaki²,

Kamijo³

et al. 2019

Acta Derm Venerol

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Fig. 1. (a) Serum activin A levels in sera of all mycosis fungoides (MF)/Sézary syndrome (SS) (n = 29) patients and those of normal controls (n = 14). p = 0.012 by Mann-Whitney test. (b) Serum activin A levels in sera of stage I or IIA of MF/SS (early stage of MF/SS; n = 8) patients, those of stage IIB or more of patients with MF/SS (advanced stage of MF/SS; n = 21), and those of normal controls (n = 14). p = 0.004 by Kruskal-Wallis test. The measured values from individual patients were plotted by dots. Error bars represent standard error of the mean. (c) Correlation between serum activin A levels and serum soluble interleukin (IL)-2 receptor levels in MF/SS (n = 28). p = 0.005 by Spearman's rank correlation test. (d) Correlation between serum activin A levels and serum lactate dehydrogenase levels in MF/SS (n = 29). p = 0.014 by Spearman's rank correlation test.

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Section: Discussionsupporting

confidence: 90%

Increased Serum Activin A Levels in Mycosis Fungoides and Sézary Syndrome

Oka

Miyagaki²,

Kamijo³

et al. 2019

Acta Derm Venerol

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“…As an important member of the TGF-β, INHBA is associated with tumorigenesis and tumor progression in multiple types of solid tumors. Si et al found that high expression of INHBA is an adverse prognostic factor for de novo acute myeloid leukemia [60] . Wamsley et al demonstrates that INHBA mRNA and protein expression are elevated in human non-small cell lung cancer (NSCLC) and INHBA is a critical autocrine factor that matains mesenchymal properties of cancer-initiating cells in NSCLC [61] .…”

Section: Discussionmentioning

confidence: 99%

Identification of INHBA as a Potential Biomarker for Gastric Cancer by Comprehensive Analysis

LIU

Jia

et al. 2020

Preprint

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BackgroundInhibin subunit beta A (INHBA) is a member of the TGF-beta (transforming growth factor-beta) superfamily proteins, which plays a fundamental role in various cancers. However, there is little systematical analysis on the exact role of INHBA in patients with gastric cancer (GC). Herein, we explored the exact role and the underlying mechanisms of INHBA regarding GC using multiple bioinformatic approaches. MethodsThe expression levels of INHBA in GC were analyzed in TIMER, GEPIA2, GEO, Oncomine and UALCAN databases. Protein and PCR test were performed to verify the expression states of INHBA in GC tissues. The correlation of INHBA and prognosis of GC was analyzed based on Kaplan Meier plotter database. Besides, the relationship between INHBA expression and immune infiltration levels and the type markers of immune cells in GC was explored in the TIMER database. What’s more, we studied INHBA mutations, promoter methylation, functional enrichment analysis in GC patients based on cBioportal, MEXPRESS, Metascape and LinkedOmics databases. Besides, we performed immunohistochemistry (IHC) and polymerase chain reaction (qRT-PCR ) verification in tissues from patients with gastric cancerResultsINHBA was elevated in GC and high expression level of INHBA in GC was significantly related to the unfavorable prognosis. Protein and PCR test verified the highly expression states of INHBA in GC tissues. Further analysis showed that INHBA was negatively correlated with B cell while positively correlated with the marker type of CD8+ T cells, macrophage, neutrophil and dendritic cell infiltration. High INHBA expression level had a poor prognosis in different enriched immune cells subgroups in GC. And there is week significant methylation level change between tumor and normal tissues. Moreover, INHBA mainly enriched cancer-related signaling pathways, including TGF-beta signaling pathway, ECM-receptor signaling pathway, PID ALK1/2 pathway, and AGE-RAGE signaling pathway. ConclusionsThe present study implies that INHBA may serve as a potential biomarkers for predicting prognosis in GC patients. High INHBA expression in GC may affect prognosis through immune infiltration.

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“…Han et al [16] reported that RNAi could inhibit virus production and effectively inhibit pre-and/or post-integration infection events in the HIV-1 life cycle. Si et al [4] study showed that the H460 cells at 70% confluence was transfected using the optimal small interfering (siRNA) and the INHBA knockdown efficiency was 93% by the quantitative RT-PCR and the concentration of INHBAtargeting siRNA reduced 6% after 96 h of transfection. However, in the study, we constructed four inducible INHBA expression vectors as the experiment groups contain I-1, I-2, I-3, I-4, and an empty vector PLLU2G as the negative control group.…”

Section: Discussionmentioning

confidence: 99%

“…Activins are formed by the heterodimeric combinations of the two subunits (activin A =βA-βA, activin AB =βA-βB and activin B =βB-βB) [3] . Inhibin inhibits FSH secretion from the anterior pituitary and in turn regulates gonadal function and development [4] . And the mutations of INHBA gene effects on litter size in sheep significantly [5] , and plasma inhibin concentrations was related to higher ovulation rates in pigs during follicular phase [6] .…”

Section: Introductionmentioning

confidence: 99%

Üreme Mevsimi Dışında Kazak Koyunlarının Üremeleri Üzerine İnhibin-βA Geninin Etkisi

Zhu¹,

Xie²,

Yang³

et al. 2019

Kafkas Univ Vet Fak Derg

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Effect of inhibin-βA subunit gene on reproductive performance of Kazakh sheep in non-breeding seasons. Kafkas Univ Vet Fak Derg, 25 (5): 611-618, 2019.Abstract Inhibin-βA (INHBA) could feedback suppress synthesis and secretion of follicle-stimulating hormone (FSH), which correlates with the litter size of sheep. In this study, the inhibin-βA subunit was used as a candidate gene, and Kazakh sheep was used as a test object. The polymorphism of the gene was detected by PCR-SSCP method and its association with litter size was analyzed. The results showed that there were three polymorphisms in the exon 5'UTR region (primer 0-2), for which AA, AB and BB were detected in Kazakh sheep. The correlation analysis showed that genotype AA had 0.13 (P<0.05) lambs and 0.16(P<0.05) lambs more than genotype AB and BB in Kazakh sheep. Vectors that interfering the INHBA expression including PLLU2G-shINHBA-1 (I-1), PLLU2G-shINHBA-2 (I-2), PLLU2G-shINHBA-3 (I-3) and PLLU2G-INHBA-4 (I-4) were constructed by RNA interference (RNAi) technology in the study. After the successfully separated ovarian granulosa cells were transfected with the vector, and the expression level of the gene was detected by quantitative RT-PCR. The results showed that the four vectors suppressed INHBA mRNA levels with a silencing efficiency of 34%, 58%, 39% and 19% respectively, with better interference efficiency of I-2. Then, we determined the contents of INHBA, FSH, luteinizing hormone (LH) and estradiol (E2) in serum by directing intro-ovarian injection of the I-2. The results showed that the INHBA level dropped and the FSH level raised in serum, while LH and E2 levels did not change, indicating the RNAi vector could successfully silence the INHBA expression in vivo. This study sets a good theoretical basis of researching the breeding and estrus properties of sheep, and the short hairpin (shRNA) vector is hopefully used in promoting the fecundity of sheep in practice. Özİnhibin-βA (INHBA), folikül stimule edici hormon (FSH) sentez ve salınımını baskılayabilir ki bu durum koyunlarda yavru sayısı ile ilişkili olabilir. Bu çalışmada, inhibin-βA geni aday gen olarak değerlendirilerek Kazak koyunları çalışma materyali olarak kullanıldı. Genin polimorfizmi PCR-SSCP metodu ile belirlendi ve yavru sayısı ile ilişkisi analiz edildi. Elde edilen sonuçlar, Kazak koyununda ekzon 5'UTR bölgesinde AA, AB ve BB için üç polimorfizmin bulunduğunu gösterdi (primer 0-2). Korelasyon analizi, Kazak koyununda genotip AA'da 0.13 kuzu bulunduğunu (P<0.05) ve AA genotipinde AB ve BB genotiplerinden 0.16 daha fazla (P<0.05) kuzu bulunduğun gösterdi. Bu çalışmada INHBA ekspresyonuna etkiyen PLLU2G-shINHBA-1 (I-1), PLLU2G-shINHBA-2 (I-2), PLLU2G-shINHBA-3 (I-3) ve PLLU2G-INHBA-4 (I-4)'ü içeren vektörler, RNA interferans (RNAi) teknolojisi ile oluşturuldu. Başarıyla ayrılan ovaryum granuloza hücreleri vektörler ile transfekte edildi ve gen ekspresyon seviyesi kantitatif RT-PCR ile belirlendi. Sonuçlar dört vektörün, sırasıyla %34, %58, %39 ve %19 gen susturma etkinlikleri ile INHBA mRN...

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High expression of INHBA is an adverse prognostic factor forde novoacute myeloid leukemia

Cited by 13 publications

References 35 publications

Increased Serum Activin A Levels in Mycosis Fungoides and Sézary Syndrome

Increased Serum Activin A Levels in Mycosis Fungoides and Sézary Syndrome

Identification of INHBA as a Potential Biomarker for Gastric Cancer by Comprehensive Analysis

Üreme Mevsimi Dışında Kazak Koyunlarının Üremeleri Üzerine İnhibin-βA Geninin Etkisi

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