High expression of IQGAP3 indicates poor prognosis in colorectal cancer patients

Wu, Jian; Chen, Zhe; Cao, Huihua; Yu, Zhan; Jin, Feng; Wang, Kai; Lu, Qicheng; Wu, Yugang

doi:10.1177/1724600819876951

Cited by 16 publications

(12 citation statements)

References 20 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CRC is one of the most dangerous cancers and its global incidence and mortality have increased markedly over the past decades. Though genetic and epigenetic changes of CRC have been broadly reported, the molecular mechanisms in the development of CRC remain to be further explored [ 29 ]. We aim to investigate the role of CRCSC-derived exosomal miR-17-5p during CRC development with the involvement of SPOP.…”

Section: Discussionmentioning

confidence: 99%

Tumor stem cell-derived exosomal microRNA-17-5p inhibits anti-tumor immunity in colorectal cancer via targeting SPOP and overexpressing PD-L1

Sun

Cui

et al. 2022

Cell Death Discov.

View full text Add to dashboard Cite

Exosomes are known to transmit microRNAs (miRNAs) to affect human cancer progression, and miR-17-5p has been manifested to exert facilitated effects on colorectal cancer (CRC) progression, while the role of tumor stem cells-derived exosomal miR-17-5p in CRC remains unknown. We aim to explore the effect of CRC stem cells-derived exosomes (CRCSC-exos) conveying miR-17-5p on CRC. The exosomes were isolated from CRC stem cells and identified. HCT116 cells were transfected with speckle-type POZ protein (SPOP) interfering vector or co-cultured with exosomes carrying miR-17-5p mimic/inhibitor. Then, the proliferation, migration, invasion, and apoptosis of the cells were determined. The xenograft mouse model was constructed using BALB/C mice and the serum levels of T cell cytokines were assessed. Expression of miR-17-5p, SPOP, CD4, CD8 and programmed death ligand 1 (PD-L1) was detected. The targeting relationship between miR-17-5p and SPOP was verified. MiR-17-5p was upregulated and SPOP was downregulated in CRC tissues. CRCSC-exos transmitted miR-17-5p to HCT116 cells to promote malignant behaviors and suppress anti-tumor immunity of HCT116 cells. The overexpressed SPOP exerted opposite effects. SPOP was confirmed as a target gene of miR-17-5p. Upregulated CRCSC-exosomal miR-17-5p inhibits SPOP to promote tumor cell growth and dampen anti-tumor immunity in CRC through promoting PD-L1.

show abstract

Section: Discussionmentioning

confidence: 99%

Tumor stem cell-derived exosomal microRNA-17-5p inhibits anti-tumor immunity in colorectal cancer via targeting SPOP and overexpressing PD-L1

Sun

Cui

et al. 2022

Cell Death Discov.

View full text Add to dashboard Cite

show abstract

“…Zhang et al found that IQGAP3 had high expression in breast cancer tissues and cell lines, and its high expression was correlated with the clinical stage, tumor node metastasis stage, and a poor prognosis ( Hua et al, 2020 ). High expression of IQGAP3 has been observed in colorectal cancer ( Wu et al, 2019 ), hepatocellular carcinoma ( Shi et al, 2017 ), bladder cancer ( Xu et al, 2019 ), and gastric cancer ( Huang et al, 2021 ). Hence, IQGAP3 appears to have important roles in cancer progression and could be a promising biomarker.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analyses of the Immunological and Prognostic Roles of an IQGAP3AR/let-7c-5p/IQGAP3 Axis in Different Types of Human Cancer

Yuan

Jiang

Tang

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

IQ motif containing GTPase-activating protein 3 (IQGAP3) is a member of the Rho family of guanosine-5′-triphosphatases (GTPases). IQGAP3 plays a crucial part in the development and progression of several types of cancer. However, the prognostic, upstream-regulatory, and immunological roles of IQGAP3 in human cancer types are not known. We found that IQGAP3 expression was increased in different types of human cancer. The high expression of IQGAP3 was correlated with tumor stage, lymph node metastasis, and a poor prognosis in diverse types of human cancer. The DNA methylation of IQGAP3 was highly and negatively correlated with IQGAP3 expression in diverse cancer types. High DNA methylation in IQGAP3 was correlated with better overall survival in human cancer types. High mRNA expression of IQGAP3 was associated with tumor mutational burden, microsatellite instability, immune cell infiltration, and immune modulators. Analyses of signaling pathway enrichment showed that IQGAP3 was involved in the cell cycle. IQGAP3 expression was associated with sensitivity to a wide array of drugs in cancer cells lines. We revealed that polypyrimidine tract–binding protein 1 (PTBP1) and an IQGAP3-associated lncRNA (IQGAP3AR)/let-7c-5p axis were potential regulations for IQGAP3 expression. We provided the first evidence to show that an IQGAP3AR/let-7c-5p/IQGAP3 axis has indispensable roles in the progression and immune response in different types of human cancer.

show abstract

“…IQGAP3 has also previously been implicated in the proliferation of epithelial cells (27). Moreover, previous studies have revealed the role of IQGAP3 in the proliferation and metastasis of lung, gastric, breast, pancreatic cancer, and colorectal cancer as well as hepatocellular carcinoma (29)(30)(31)(32)(33)(34)(35). Therefore, the role of IQGAP3 is crucial in the malignant transformation of several types of cancer.…”

Section: Discussionmentioning

confidence: 98%

“…Moreover, IQGAP3 is hypothesized to be involved in the proliferation of epithelial cells (27), and is a novel member of the IQGAP family, which was discovered in 2007 (28). IQGAP3 is located on chromosome 1 at 1q21.3 loci and has been reported to act as an oncogene in several types of cancer (29)(30)(31)(32)(33)(34)(35). Furthermore, IQGAP3 is a transmembrane protein, and has been speculated to be a potential therapeutic target (35).…”

Section: Iqgap3 Promotes Cancer Proliferation and Metastasis In High-grade Serous Ovarian Cancermentioning

confidence: 99%

IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer

et al. 2020

View full text Add to dashboard Cite

Ovarian cancer is a type of gynecological cancer with the highest mortality rate worldwide. Due to a lack of effective screening methods, most cases are diagnosed at later stages where the survival rates are poor. Thus, it is termed a ‘silent killer’ and is the most lethal of all the malignancies in women. IQ motif containing GTPase Activating Protein 3 ( IQGAP3 ) is a member of the Rho family of GTPases, and plays a crucial role in the development and progression of several types of cancer. The aim of the present study was to investigate the oncogenic functions and mechanisms of IQGAP3 on the proliferation and metastasis of high-grade serous ovarian cancer (HGSOC). Therefore, the expression levels of IQGAP3 in HGSOC and normal tissue samples were compared, and IQGAP3 knockdown was performed to examine its functional role using various in vitro and in vivo experiments. It was demonstrated that the expression of IQGAP3 was upregulated in HGSOC tissues compared with the healthy tissues; this differential expression was also observed in the ovarian cancer cell lines. Functional experimental results suggested that IQGAP3 silencing significantly reduced proliferation, migration and invasion in ovarian cancer cell lines. Moreover, in vivo experimental findings validated the in vitro results, where the tumorigenic and metastatic capacities of IQGAP3 -silenced cells were significantly lower in the nude mice compared with the mice implanted with the control cells. Furthermore, knockdown of IQGAP3 resulted in increased apoptosis, and the effects of IQGAP3 expression on various epithelial-mesenchymal transition markers were identified, suggesting a possible mechanism associated with the role of IQGAP3 in metastasis. The effect of IQGAP3 silencing on chemosensitivity towards olaparib was also assessed. Collectively, the present results indicated that IQGAP3 is a potential diagnostic and prognostic marker, and a putative therapeutic target of HGSOC.

show abstract

High expression of IQGAP3 indicates poor prognosis in colorectal cancer patients

Cited by 16 publications

References 20 publications

Tumor stem cell-derived exosomal microRNA-17-5p inhibits anti-tumor immunity in colorectal cancer via targeting SPOP and overexpressing PD-L1

Tumor stem cell-derived exosomal microRNA-17-5p inhibits anti-tumor immunity in colorectal cancer via targeting SPOP and overexpressing PD-L1

Comprehensive Analyses of the Immunological and Prognostic Roles of an IQGAP3AR/let-7c-5p/IQGAP3 Axis in Different Types of Human Cancer

IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer

Contact Info

Product

Resources

About