High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer

Selven, Hallgeir; Andersen, Sigve; Pedersen, Mona Irene; Lombardi, Ana Paola G.; Busund, Lill-Tove; Kilvaer, Thomas K.

doi:10.1038/s41598-022-11090-2

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…On the other hand, higher expression levels of miR-19a-3p and miR-20a-5p were observed in the low-risk group. That is in agreement with the results of Selven et al who showed a favorable prognosis for colorectal cancer patients with high miR-20a-5p expression [ 33 ]. Similarly, Farzadfard et al reported higher circulating miR-19a-3p in CLL patients than in healthy subjects [ 2 ].…”

Section: Discussionsupporting

confidence: 93%

“…We also demonstrated higher miR-20a-5p and miR-92a-1-5p expression levels in CLL patients with CR and/or PR than in patients who died. The results of the current study are consistent with those of Selven et al that have shown that high expression levels of miR-20a-5p in tumor tissue are independent indicators of favorable disease-specific survival in colon cancer [ 33 ]. Likewise, Papageorgiou et al reported that high miR-92a-3p expression predicts significantly prolonged OS (overall survival) of CLL patients [ 10 ].…”

Section: Discussionsupporting

confidence: 92%

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Prognostic Value of the miR-17~92 Cluster in Chronic Lymphocytic Leukemia

Chocholska

Zarobkiewicz

Szymańska

et al. 2023

IJMS

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The aim of this study was to investigate the expression of miR-17∼92 cluster members in chronic lymphocytic leukemia (CLL) patients. Six microRNAs (miRNAs)—miR-17, miR-18a, miR-19a, miR-19b-1, miR-20a, and miR-92a-1—very poorly characterized in CLL patients, were chosen for the study to consider their possible role as cancer biomarkers. It is currently unclear to which extent miR-17~92 expression is related to other routinely measured CLL markers, and whether the findings can be of any clinical significance. To achieve this goal, we report the expression levels of these miRNAs detected by RT-qPCR in purified CD19+ B lymphocytes of 107 CLL patients and correlate them with existing clinical data. The study provides new evidence regarding the heterogeneity of miR-17~92 cluster members’ expression in CLL patients. Higher miR-17-5p expression was associated with unfavorable prognostic factors (i.e., 17p and 11q deletions, CD38 and ZAP-70 expression). On the other hand, miR-19a, miR-20a, and miR-92a-1 negatively correlated with these adverse factors. The presence of del(13q) as a sole aberration was associated with a significantly lower miR-17-5p as well as higher miR-19a-3p and miR-92a-1-5p expression compared to patients carrying unfavorable genetic aberrations. Particularly, miR-20a could be considered an independent favorable prognostic factor. In a multivariate analysis, high miR-20a expression remained an independent marker predicting long TTT (time to treatment) for CLL patients.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Prognostic Value of the miR-17~92 Cluster in Chronic Lymphocytic Leukemia

Chocholska

Zarobkiewicz

Szymańska

et al. 2023

IJMS

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“…1 ). Consistent with our results, Selven et al also demonstrated that high expression levels of miR-20a-5p are positively correlated with a favorable prognosis in advanced stages of CRC [ 37 ]. Additionally, miR-122-5p has been identified to promote CRC progression [ 38 ].…”

Section: Discussionsupporting

confidence: 92%

Cancer-associated fibroblasts drive colorectal cancer cell progression through exosomal miR-20a-5p-mediated targeting of PTEN and stimulating interleukin-6 production

Ghofrani-Shahpar,

Pakravan,

Razmara

et al. 2024

BMC Cancer

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Background This study evaluated the clinical relevance of a set of five serum-derived circulating microRNAs (miRNAs) in colorectal cancer (CRC). Additionally, we investigated the role of miR-20a-5p released by exosomes derived from cancer-associated fibroblasts (CAFs) in the context of CRC. Methods The expression levels of five circulating serum-derived miRNAs (miR-20a-5p, miR-122-5p, miR-139-3p, miR-143-5p, and miR-193a-5p) were quantified by real-time quantitative PCR (RT-qPCR), and their associations with clinicopathological characteristics in CRC patients were assessed. The diagnostic accuracy of these miRNAs was determined through Receiver Operating Characteristic (ROC) curve analysis. CAFs and normal fibroblasts (NFs) were isolated from tissue samples, and subsequently, exosomes derived from these cells were isolated and meticulously characterized using electron microscopy and Western blotting. The cellular internalization of fluorescent-labeled exosomes was visualized by confocal microscopy. Gain- and loss-of-function experiments were conducted to elucidate the oncogenic role of miR-20a-5p transferred by exosomes derived from CAFs in CRC progression. The underlying mechanisms were uncovered through luciferase reporter assay, Western blotting, enzyme-linked immunosorbent assays, as well as proliferation and migration assays. Results The expression levels of serum-derived circulating miR-20a-5p and miR-122-5p were significantly higher in CRC and were positively correlated with advanced stages of tumorigenesis and lymph node metastasis (LNM). In contrast, circulating miR-139-3p, miR-143-5p, and miR-193a-5p were down-regulated in CRC and associated with early tumorigenesis. Except for miR-139-3p, they showed a negative correlation with LNM status. Among the candidate miRNAs, significantly elevated levels of miR-20a-5p were observed in both cellular and exosomal fractions of CAFs. Our findings indicated that miR-20a-5p induces the expression of EMT markers, partly by targeting PTEN. Exosomal miR-20a secreted by CAFs emerged as a key factor enhancing the proliferation and migration of CRC cells. The inhibition of miR-20a impaired the proliferative and migratory potential of CAF-derived exosomes in SW480 CRC cells, suggesting that the oncogenic effects of CAF-derived exosomes are mediated through the exosomal transfer of miR-20a. Furthermore, exosomes originating from CAFs induced increased nuclear translocation of the NF-kB p65 transcription factor in SW480 CRC cells, leading to increased interleukin-6 (IL-6) production. Conclusions We established a set of five circulating miRNAs as a non-invasive biomarker for CRC diagnosis. Additionally, our findings shed light on the intricate mechanisms underpinning the oncogenic impacts of CAF-derived exosomes and underscore the pivotal role of miR-20a-5p in CRC progression.

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“…The higher expression of these miRNAs also correlated with negative ZAP70 and CD38 expression, while low miR-20a expression (<1.629) was strongly associated with shorter time to treatment (TTT) and PD. In multivariate analysis, low miR-20a expression remained an independent marker predicting short TTT for CLL patients, replying to the results obtained by Selven and colleagues [81] in colorectal cancer and opening a way to its use as a potential blood biomarker.…”

Section: Mir-17-92 Clustermentioning

confidence: 68%

Role of microRNAs in Chronic Lymphocytic Leukemia

Autore

Ramassone

Stirparo

et al. 2023

IJMS

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Chronic Lymphocytic Leukemia (CLL) is the most common form of leukemia in adults, with a highly variable clinical course. Improvement in the knowledge of the molecular pathways behind this disease has led to the development of increasingly specific therapies, such as BCR signaling inhibitors and BCL-2 inhibitors. In this context, the emerging role of microRNAs (miRNAs) in CLL pathophysiology and their possible application in therapy is worth noting. MiRNAs are one of the most important regulatory molecules of gene expression. In CLL, they can act both as oncogenes and tumor suppressor genes, and the deregulation of specific miRNAs has been associated with prognosis, progression, and drug resistance. In this review, we describe the role of the miRNAs that primarily impact the disease, and how these miRNAs could be used as therapeutic tools. Certainly, the use of miRNAs in clinical practice is still limited in CLL. Many issues still need to be solved, particularly regarding their biological and safety profile, even if several studies have suggested their efficacy on the disease, alone or in combination with other drugs.

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High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer

Cited by 11 publications

References 43 publications

Prognostic Value of the miR-17~92 Cluster in Chronic Lymphocytic Leukemia

Prognostic Value of the miR-17~92 Cluster in Chronic Lymphocytic Leukemia

Cancer-associated fibroblasts drive colorectal cancer cell progression through exosomal miR-20a-5p-mediated targeting of PTEN and stimulating interleukin-6 production

Role of microRNAs in Chronic Lymphocytic Leukemia

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