High expression of RUVBL1 and HNRNPU is associated with poor overall survival in stage I and II non-small cell lung cancer patients

Abstract

The present study aimed to investigate expression levels and prognostic significance of RUVBL1 and HNRNPU in stage I and II non–small-cell lung cancer (NSCLC) patients. Therefore, we evaluated immunohistochemical staining of RUVBL1 and HNRNPU, as well as RNA-seq data from public sources, and the results were evaluated concerning overall survival (OS) and clinicopathological features. We found that RUVBL1 and HNRNPU proteins and mRNA levels were higher in tumor tissues as compared to adjacent/normal tissues. RUVBL1 (p = 0.013) and HNRNPU (p = 0.021) high protein levels were independent prognostic factors for poor OS. Also, the multivariate analysis in the TCGA dataset revealed that high RUVBL1 (p = 0.064) and HNRNPU (p = 0.181) mRNA levels were not significantly associated with prognosis. However, the co-expression status of these markers (R + H +) was independently associated with poor OS both in the TCGA dataset (p = 0.027) and in our cohort (p = 0.001). In conclusion, combined and individual expression of RUVBL1 and HNRNPU proteins, as well as R + H + mRNA status, may serve as potential prognostic biomarkers for NSCLC. This study adds to the previous observations that RUVBL1 and HNRNPU might be novel and promising therapeutic targets and markers for prognostic evaluation.

RUVBL1-modulated chromatin remodeling alters the transcriptional activity of oncogenic CTNNB1 in uveal melanoma

“…The initial finding from this study supported that RUVBL1 was expressed at a high level in UVM samples and cells, acting as an independent prognostic factor. Largely in agreement with our finding, highly expressed RUVBL1 in non-small cell lung cancer has been reported to be indicative of undesirable oncogenic outcomes of patients, serving as a promising prognostic biomarker [ 17 ]. Also, the prognostic and diagnostic potential of RUVBL1 in oral squamous cell carcinoma has been uncovered [ 18 ].…”

RUVBL1 in Clear-Cell Renal Cell Carcinoma: Unraveling Prognostic Significance and Correlation with HIF1A

“…Our analyses, in line with the findings in the TCGA cohort, revealed a substantial upregulation in the protein expression of RUVBL1 in ccRCC tissues when compared to the corresponding control tissues. These discoveries are consistent with previous studies, which also noted the elevated expression of RUVBL1 in other cancer types, such as oral squamous cell carcinoma [15], osteosarcoma [16], salivary gland cancer [17], lung cancer [18,19], hepatocellular carcinoma [20], colorectal carcinoma [21,22], and glioma [23]. In the study conducted by Zhang et al, they also observed strong cytoplasmic and nuclear staining of RUVBL1 in ccRCC tissues, whereas non-tumor tissues exhibited either no expression of RUVBL1 or predominantly moderate nuclear expression [24].…”

“…Our findings bolstered the idea that both forms of expression are intricately associated with a shorter survival time, offering additional insights into the multifaceted role of RUVBL1 in the context of ccRCC. The association between high expression levels and a shorter survival time has also been observed in studies conducted by other authors [17,19,[23][24][25][26]. The cumulative evidence robustly underscores the prospective clinical significance of RUVBL1 as a prognostic marker in ccRCC, underscoring its importance in forecasting the overall survival of patients.…”