High-fat diets rich in soy or fish oil distinctly alter hypothalamic insulin signaling in rats

Pimentel, Gustavo D.; Dornellas, Ana Paula Segantine; Rosa, José César; Lira, Fábio Santos; Cunha, Carolina; Boldarine, Valter Tadeu; Souza, Gabriel I.H.; Hirata, Aparecida Emiko; Nascimento, Cláudia Maria Oller do; Oyama, Lila Missae; Watanabe, Regina Lúcia Harumi; Ribeiro, Eliane

doi:10.1016/j.jnutbio.2011.04.006

Cited by 26 publications

(16 citation statements)

References 43 publications

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“…These findings agree with the report of induction of inflammatory process after intracerebroventricular administration of the n-6 PUFA linoleic acid but not of the n-3 PUFA linolenic acid to rats for 3 days [6]. Interestingly, unlike the effects of saturated fat-feeding [6–9], in the present experiments, the levels of TNF- α , IL-1 β , and IL-6, as well as of their receptor proteins, were not altered in the hypothalamus of the soy-diet rats, in agreement with previous data [6]. Intermediates of the NF κ B pathway have been shown to affect insulin signal transmission, as IKK was able to both induce serine phosphorylation of IRS-1 and upregulate SOCS3 (suppressor of cytokine signaling) in the hypothalamus of mice fed high-fat saturated diet [19].…”

Section: Discussionsupporting

confidence: 91%

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High-Fat Fish Oil Diet Prevents Hypothalamic Inflammatory Profile in Rats

et al. 2013

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Whether PUFA diets affect inflammatory mediators in central and peripheral sites is not clear. We investigated the effect of high-fat PUFA diets on the expression of proteins involved in inflammatory pathways in hypothalamus, muscle, and liver. Male rats were fed for 2 months with either chow or high-fat diets enriched with either soy (n-6 PUFAs) or fish oil (n-3 PUFAs). The fish group had normal body weight, low serum NEFA, reduced hypothalamic levels of TNF-α, IL-6, and TRAF6, and increased levels of IL-10 receptor. In contrast, the soy group had increased body weight and hypothalamic levels of TRAF6 and NFκBp65. In muscle, the fish diet reduced TNF-α and IL-6 levels. Both PUFA diets increased muscle IL-10 levels and reduced liver TNF-α and IL-6 levels. The data showed that the high-fat soy diet induced activation of the hypothalamic NFκB inflammatory pathway, a feature predisposing to feeding and energy expenditure disturbances associated with the development of obesity. On the other hand, the high-fat fish diet improved the central and the peripheral inflammatory profile via reduction of intracellular inflammatory mediators, suggesting a protection against obesity.

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Section: Discussionsupporting

confidence: 91%

“…Serum endotoxin was assayed using chromogenic limulus amebocyte lysate kit (Cambrex Bio Science, MD, USA), as previously detailed [9]. …”

Section: Methodsmentioning

confidence: 99%

High-Fat Fish Oil Diet Prevents Hypothalamic Inflammatory Profile in Rats

et al. 2013

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“…Impaired insulin-induced hypophagia, as well as impaired hypothalamic insulin signalling, have been shown in adult rats submitted to intrauterine food restriction, in rats fed a trans fatty acid-enriched, and in rats fed a soybean oil-enriched diet (4,25,59). …”

Section: We Did Not Find Any Differences In Hypothalamic Levels Of Irmentioning

confidence: 99%

“…In the hypothalamus, insulin binds to insulin receptors present in areas involved in the control of food intake, including the arcuate nucleus and the ventromedial hypothalamus (3,4,5). The initial steps of insulin signalling in hypothalamic nuclei are similar to those in muscle and adipose tissues (6,7).…”

Section: Introductionmentioning

confidence: 99%

Deleterious effects of lard-enriched diet on tissues fatty acids composition and hypothalamic insulin actions

Dornellas

Watanabe

Pimentel

et al. 2015

Prostaglandins, Leukotrienes and Essential Fatty Acids

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“…Deol et al reported that an HFD (C:F:P = 43:40:16) containing soybean oil and hydrogenated coconut oil at 1:1 ratio was more obesogenic than an HFD mainly containing hydrogenated coconut oil [10]. These differences were considered to be caused by the lipid composition of the dietary fat [1, 8, 12, 17, 32, 34]. Polyunsaturated FAs (PUFAs) are the main contributors to the physiological activity of dietary fat; soybean oil contains 15% saturated FAs and 55% PUFAs, while lard contains 40% saturated FAs and 10% PUFAs.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

Tanaka¹,

Yasuoka

Shimizu

et al. 2017

Genes Nutr

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BackgroundTo elucidate the effects of altered dietary carbohydrate and fat balance on liver and adipose tissue transcriptomes, 3-week-old rats were fed three kinds of diets: low-, moderate-, and high-fat diets (L, M, and H) containing a different ratio of carbohydrate-fat (C-F) (65:15, 60:20, and 35:45 in energy percent, respectively).MethodsThe rats consumed the diets for 9 weeks and were subjected to biochemical and DNA microarray analyses.ResultsThe rats in the H-group exhibited lower serum triacylglycerol (TG) levels but higher liver TG and cholesterol content than rats in the L-group. The analysis of differentially expressed genes (DEGs) between each group (L vs M, M vs H, and L vs H) in the liver revealed about 35% of L vs H DEGs that were regulated in the same way as M vs H DEGs, and most of the others were L- vs H-specific. Gene ontology analysis of these L vs H DEGs indicated that those related to fatty acid synthesis and circadian rhythm were enriched. Interestingly, about 30% of L vs M DEGs were regulated in a reverse way compared with L vs H and M vs H DEGs. These reversed liver DEGs included M-up/H-down genes (Sds for gluconeogenesis from amino acids) and M-down/H-up genes (Gpd2 for gluconeogenesis from glycerol, Agpat9 for TG synthesis, and Acot1 for beta-oxidation). We also analyzed L vs H DEGs in white (WAT) and brown (BAT) adipose tissues and found that both oxidation and synthesis of fatty acids were inhibited in these tissues.ConclusionsThese results indicate that the alteration of dietary C-F balance differentially affects the transcriptomes of metabolizing and energy-storing tissues.Electronic supplementary materialThe online version of this article (doi:10.1186/s12263-017-0558-2) contains supplementary material, which is available to authorized users.

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High-fat diets rich in soy or fish oil distinctly alter hypothalamic insulin signaling in rats

Cited by 26 publications

References 43 publications

High-Fat Fish Oil Diet Prevents Hypothalamic Inflammatory Profile in Rats

High-Fat Fish Oil Diet Prevents Hypothalamic Inflammatory Profile in Rats

Deleterious effects of lard-enriched diet on tissues fatty acids composition and hypothalamic insulin actions

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

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