High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat

Akiyama, Takahiko; Tachibana, Issei; Shirohara, Hisashi; Watanabe, Nobuaki; Ōtsuki, Makoto

doi:10.1016/0168-8227(96)01205-3

Cited by 151 publications

(110 citation statements)

References 36 publications

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“…Numerous reports have focused on the role of high fat diets on insulin action in skeletal muscle [21,22,23,24,25]. Consistently, it has been reported that high fat diet increases the proportion of saturated fatty acids in the skeletal muscle membranes.…”

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confidence: 86%

Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle

et al. 2003

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Aim/hypothesis. Previous studies have shown that prolonged glucose infusion causes insulin resistance and triglyceride accumulation in rat skeletal muscle. In this study, we investigated a possible relationship between insulin resistance and the composition of different accumulated lipid fractions in rat skeletal muscle. Methods. Continuous glucose infusion was carried out in rats for 7 days. Lipids were extracted from skeletal muscle, separated by thin layer chromatography and fatty acid composition of phospholipids, triglycerides, diglycerides, free fatty acids and cholesterol esters fractions was analysed by gas chromatography. ∆9-Desaturase mRNA was measured by real time polymerase chain reaction. The enzyme activity was measured in the microsomal fractions. Results. Prolonged glucose infusion (5 days) increased the relative content of palmitoleic acid (16:1 N7) several-fold (2.3-to 5.8-fold) in four out of five lipid fractions and enhanced oleic acid (18:1 N9) two-fold in three lipid fractions suggesting increased ∆9-desaturase activity while the content of several polyunsaturated fatty acids was reduced. In parallel, ∆9-Desaturase mRNA contents and enzyme activities in skeletal muscle were increased 10-fold, 75-fold, 2.6-fold and 7.7-fold after 2 and 5 days of glucose infusion, respectively. Conclusion/interpretation. Our results suggest that long-term glucose oversupply induces a rapid increase in ∆9-desaturase expression and enzyme activity in skeletal muscle which leads to fast and specific changes in fatty acid metabolism possibly contributing to the insulin resistance in this animal model. [Diabetologia (2003) 46:203-212]

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confidence: 86%

Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle

et al. 2003

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“…There is a large body of evidence that rats fed HF chronically produce obesity and insulin resistance (3,4), which is similar to the effects of the western style diet on humans (5,6). We already reported that visceral obesity is an independent determinant for decreased insulin responsiveness in skeletal muscle in vitro (7).…”

Section: Introductionmentioning

confidence: 80%

“…Insulin resistance by chronic HF is known to be associated with increased visceral fat mass (3,18). However, a large body of evidence suggests that FFA is also one of the most important factors to modulate glucose metabolism (9,10,12).…”

Section: Discussionmentioning

confidence: 99%

Effect of High Fat Diet on Insulin Resistance: Dietary Fat Versus Visceral Fat Mass

Park

Kim

et al. 2001

J Korean Med Sci

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The purpose of the present study was to determine whether chronic high-fat diet (HF) induces insulin resistance independently of obesity. We randomly divided 40 rats into two groups and fed them either with a HF or with a high-carbohydrate diet (HC) for 8 weeks. Whole body glucose disappearance rate (Rd) was measured using a euglycemic hyperinsulinemic clamp. Firstly, we defined whether insulin resistance by HF was associated with obesity. Plasma glucose and triglyceride concentrations were significantly increased in HF. Rd was decreased (10.6+/-0.2 vs. 9.1+/-0.2 mg/kg/min in HC and HF, respectively) and the hepatic glucose output rate (HGO) was increased in HF (2.2+/-0.3 vs. 4.5+/-0.2 mg/kg/min in HC and HF, respectively). Rd was significantly correlated with %VF (p<0.01). These results implicate that visceral obesity is associated with insulin resistance induced by HF. In addition, to define whether dietary fat induces insulin resistance regardless of visceral obesity, we compared Rd and HGO between groups 1) after matching %VF in both groups and 2) using an ANCOVA to adjust for %VF. After matching %VF, Rd in HF was significantly decreased by 14% (p<0.001) and HGO was significantly increased by 110% (p<0.001). Furthermore, statistical analyses using an ANCOVA also showed Rd for HF was significantly decreased even after adjusting %VF. In conclusion, we suggest that dietary fat per se could induce insulin resistance in rats fed with chronic HF independently of obesity.

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“…Segundo Akiyama et al 12 , ratos alimentados com dieta hiperenergética com alto teor de gorduras podem desenvolver obesidade. Neste estudo, embora o consumo energético semanal tenha sido semelhante para os dois grupos avaliados, observa-se que os animais do grupo--experimental tiveram maior ganho de peso quando comparados aos animais do grupo-controle, como reflexo de um consumo maior de energia ao longo das oito semanas de experimento.…”

Section: I S C U S S ã Ounclassified

“…Esse aumento da glicemia provavelmente esteja associado ao aumento da quantidade de gordura corporal e também ao aumento de hormônios hiperglicemiantes: glucagon, adrenalina, cortisol e hormônio de crescimento 19,20 . Hábitos alimentares desbalanceados levaram às alterações bioquímicas séricas tais como hiperlipidemia, hiperinsulinemia e intolerância à glicose, como foi demonstrado por Akiyama et al 12 e Fernandes et al 21 . Por esses motivos, é importante conhecer a associação entre o consumo de determinados alimentos e os níveis de lipídeos séricos 22 , uma vez que a dislipidemia pode desencadear o aparecimento de aterosclerose e outras doenças.…”

Section: Estadella Et Alunclassified

Dieta hiperlipídico-proteica utilizada para emagrecimento induz obesidade em ratos

Borba¹,

Rocha²,

Silva³

et al. 2011

Rev. Nutr.

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OBJETIVO: Neste trabalho, analisa-se o efeito de dieta hiperlipídico-proteica com baixo teor de carboidrato sobre o peso corporal, peso de órgãos, consumo de ração, parâmetros bioquímicos e alterações histopatológicas no fígado de ratos. MÉTODOS: Foram utilizados 24 animais - 12 no grupo-controle e 12 no grupo-experimental - com peso médio de 160 gramas no início do experimento. Semanalmente, foram verificados o peso corporal e o consumo de ração, e ao final de oito semanas foram feitas as dosagens bioquímicas sanguíneas, pesagem de órgãos e análise histopatológica dos fígados. RESULTADOS: Os animais do grupo-experimental tiveram maior ganho de peso corporal e acumularam mais tecido adiposo que os animais do grupo-controle. Fígado, rins e baço não sofreram alterações quanto ao peso. Os animais que receberam dieta hiperlipídico-proteica tiveram um aumento na ingestão energética acumulada nas oito semanas do estudo. O grupo-experimental desenvolveu hiperglicemia e hipertrigliceridemia, aumento da fração lipoproteína de alta densidade do colesterol e da creatinina sérica quando comparado ao grupo-controle. Foi detectada esteatose hepática no grupo-experimental. CONCLUSÃO: Os resultados demonstraram que dietas pobres em carboidratos e ricas em gordura e proteínas podem acarretar alterações metabólicas prejudiciais ao organismo.

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High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat

Cited by 151 publications

References 36 publications

Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle

Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle

Effect of High Fat Diet on Insulin Resistance: Dietary Fat Versus Visceral Fat Mass

Dieta hiperlipídico-proteica utilizada para emagrecimento induz obesidade em ratos

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