High Fluorescent Lymphocyte Count Examination in Dengue Hemorrhagic Patients With Sysmex Xn-1000 Hematology Analyzer

Abstract

Sysmex XN-1000 hematology analyzer is an automated 5-part diff analyzer (eosinophils, basophils, neutrophils, lymphocytes, and monocytes). In the calculated area, the type of difference between the Sysmex hematology device and other hematology devices is Immature Granulocyte (IG), Nucleated Red Blood Cell (NRBC), and High Fluorescent Lymphocytes Count (HFLC). The cells calculated in the HFLC area are atypical lymphocytes. In patients with dengue hemorrhagic fever, it is often found atypical lymphocytes called blue plasma lymphocytes. The purpose of this study was to determine the description of HFLC in patients with dengue fever using the hematology analyzer Sysmex XN-1000. A descriptive retrospective study was conducted during April-May 2017. The subjects of the study were adult patients diagnosed with dengue hemorrhagic fever with WHO criteria. Of the 47 samples of Dengue Hemorrhagic Fever (DHF) patients, the average HFLC results were between 2.0-32.3%, which was 11.5%, while the average range of normal HFLC values was between 0.0-1.4% and was 0.3%. In cases of DHF, there is an increase in HFLC. This is likely to be attributed to atypical lymphocyte increase in dengue hemorrhagic fever. Further research with more varied samples still needs to be done.

Rapid screening of dengue fever using research parameters from new generation hematological analyzers

“…We observed significantly high HFLC% in cases with dengue fever compared with the febrile illness with negative dengue serology and healthy controls. Our findings corroborated the limited data available in the literature on the utility of HFLC in dengue fever 5,9–11 …”

“…Tanzanite et al reported that HFLC% was significantly higher in the febrile patients with infective etiologies, predominantly dengue fever (67%), compared with patients with either autoimmune diseases or malignancies or healthy controls 9 . Similarly, Raharjo et al also reported a significantly increased HFLC% in patients with dengue hemorrhagic fever 10 . In our study, the regression analysis did not find HFLC as an independent predictor for dengue fever.…”

Cross-Reaction Antibody Test between SARS-CoV-2 and Dengue Hemorrhagic Fever in Indonesia

“…Dengue virus can trigger the cellular and humoral immune system so that after the third day of fever, the anti-dengue IgM serological examination can become reactive, which is then followed by IgG. The changes in the humoral immune system can be detected through the formation of IgM and IgG antibodies that can be identified by rapid/serological 4,6,7 examination.…”

Deteksi Limfosit Plasma Biru pada Citra Darah untuk Diagnosa Pendukung pada Kasus Demam Berdarah Dengue

“…Namun deteksi dan perhitungan LBP dilakukan secara manual, diperlukan ketelitian dan pengalaman tenaga medis. Studi lainnya oleh Raharjo dan Hadi, melakukan deteksi dan perhitungan jumlah limfosit secara otomatis menggunakan Sysmex XN-1000 hematology analyzer (Raharjo & Hadi, 2019). Namun demikian, ini menjadikan ketergantungan terhadap perangkat lunak dari produsen alat tersebut.…”