High-frequency embryogenesis inBrassica campestris microspore culture

Guo, Yang-Dong; Pulli, Seppo

doi:10.1007/bf02307098

Cited by 38 publications

(24 citation statements)

References 18 publications

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“…Kott et al (1988) postulated that some toxic compounds could be released from nonembryonic microspores into the medium, which would harm potential embryogenic microspores and reduce the embryogenic frequency. So far, AC has been used with B. campestris (Guo and Pulli, 1996), B. napus (Gland et al, 1988), B. oleracea (da Silva Dias, 1999, and pepper (Supena et al, 2006;Cheng et al, 2013) to improve embryogenesis. In B. campestris, 150 mg/L AC improved embryo quality as well as the quantity of embryos by two-to fivefold.…”

Section: Discussionmentioning

confidence: 99%

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Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale

Niu¹,

Zhang²,

Tong³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. To improve embryogenesis in microspore cultures of kale (Brassica oleracea L. var. acephala DC.), 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), arabinogalactan (AG), p-chlorophenoxyisobutyric acid (PCIB), and activated charcoal (AC) were added to the medium using four varieties of kale. The results showed that the addition of AG (0.1-0.2 g/L), AC (0.1-0.2 g/L) or a combination of 6-BA (0.1-0.2 mg/L) and NAA (0.1-0.2 mg/L) promoted embryogenesis. Adding 40 μM PCIB or a combination of 40 μM PCIB and 0.2 g/L AC to NLN-13 medium at pH 5.8 effectively enhanced embryogenesis. Treatment with a combination of 40 μM PCIB and 10 mg/L AG gave the highest rate of embryonic induction, especially in genotype "Y007," which showed a twelve-fold increase in yield.

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Section: Discussionmentioning

confidence: 99%

“…In B. campestris, 150 mg/L AC improved embryo quality as well as the quantity of embryos by two-to fivefold. The enhancing effect of AC could be due to the adsorption of toxic or polyphenolic compounds produced by microspores (Guo and Pulli, 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale

Niu¹,

Zhang²,

Tong³

et al. 2015

Genet. Mol. Res.

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“…Cold pre-treatment of anthers is commonly employed to enhance the frequency of androgenic embryogenesis. It has been used extensively in many crops such as Brassica campestris (Guo and Pulli, 1996) and Triticum aestivum (Li et al, 1988). Instead of asymmetry of normal first pollen mitosis, more symmetric divisions have been reported following cold pretreatment (Kiviharju and Pehu, 1998) likely due to blocking the normal gametophytic development.…”

Section: Discussionmentioning

confidence: 99%

“…For microspore culture, sucrose concentration (w/v) at 10, 13 and 17% were tested for embryo induction across both media and six genotypes. The levels of sucrose were set according to former similar study (Guo and Pulli, 1996;Guo et al, 1999). Activated charcoal was added to the culture media at 150 mg L -1 in anther and microspore cultures.…”

Section: Experiments 4: Effects Of Basal Medium and Sugar Concentrationsmentioning

confidence: 99%

Comparison of anther and microspore culture in androgenic embryogenesis and regeneration of broccoli (Brassica oleracea L. var. italica P.)

Qin¹,

Huang²,

Pulli³

et al. 2015

Afr. J. Biotechnol.

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The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and plant regeneration for efficient doubled haploid production in broccoli breeding. Six broccoli hybrids were used in this study. Our results show that generally, the efficiency of androgenic embryogenesis and regeneration in microspore culture is higher than that in the anther culture. Moreover, the microspore culture eliminated the possibility of plantlets coming from diploid tissue. In this study, the four-day cold pre-treatment yielded the highest number of embryos in both anther and microspore culture methods; the embryo yield at 32.5°C for 24 h was the highest in anther and microspore culture. Optimal plating densities were 30 anthers per dish in anther culture and 4×10 5 microspores per ml in microspore culture. In androgenic embryo production, the PG-96 medium proved to be more effective than NLN medium. Sucrose concentration at 10% for anther culture and 13% (w/v) for microspore culture was recommended. A total of 70 regenerants were obtained from three genotypes including doubled haploids, haploids and aneuploids.

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“…However, microspore culture was not very successful in B. rapa (Guo and Pulli 1996), compared with B. napus (Zhou et al 2002;Gu et al 2004;Leroux et al 2009). Just as there are differences in embryogenic response among species, there are also genotypic differences in microspore culture response.…”

Section: Introductionmentioning

confidence: 99%

High-purity Seed Production of Doubled Haploid Chinese Cabbage [Brassica rapa L. ssp. pekinensis (Lour.)] Through Microspore Culture

Lee¹,

Lim²,

Song³

2014

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The purpose of this study was to produce doubled haploid red color heading type Chinese cabbage using isolated microspore culture. Genotypic differences in embryogenic response and regenerative ability of microspore-derived embryos to plants were observed. A high rate of plant regeneration from microspore-derived embryos was achieved by an improved protocol involving replacement of culture media and adjustment of heat shock temperature. More than 60% of regenerated plants were spontaneous doubled haploids. Haploids were characterized by short and malformed stamen and few viable pollen grains as compared to spontaneous doubled haploids and dihaploids. The seeds harvested from the 142 spontaneous doubled haploid plants were designated as H1; 25 seeds in each population was grown and characterized. The H1 generation has been shown to display considerable phenotypic variation and high purity. These spontaneous doubled haploids may be directly exploited as new varieties in a red colored Chinese cabbage breeding program.

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High-frequency embryogenesis inBrassica campestris microspore culture

Cited by 38 publications

References 18 publications

Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale

Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale

Comparison of anther and microspore culture in androgenic embryogenesis and regeneration of broccoli (Brassica oleracea L. var. italica P.)

High-purity Seed Production of Doubled Haploid Chinese Cabbage [Brassica rapa L. ssp. pekinensis (Lour.)] Through Microspore Culture

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