High-purity Seed Production of Doubled Haploid Chinese Cabbage [Brassica rapa L. ssp. pekinensis (Lour.)] Through Microspore Culture

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The purpose of this study was to produce doubled haploid red color heading type Chinese cabbage using isolated microspore culture. Genotypic differences in embryogenic response and regenerative ability of microspore-derived embryos to plants were observed. A high rate of plant regeneration from microspore-derived embryos was achieved by an improved protocol involving replacement of culture media and adjustment of heat shock temperature. More than 60% of regenerated plants were spontaneous doubled haploids. Haploids were characterized by short and malformed stamen and few viable pollen grains as compared to spontaneous doubled haploids and dihaploids. The seeds harvested from the 142 spontaneous doubled haploid plants were designated as H1; 25 seeds in each population was grown and characterized. The H1 generation has been shown to display considerable phenotypic variation and high purity. These spontaneous doubled haploids may be directly exploited as new varieties in a red colored Chinese cabbage breeding program.

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

“…В настоящее время проводится активная работа по изучению культур изолированных протопластов, их выделению и слиянию, а также использованию в селекции растений (Samko, Snigireva, 2009;Shen et al, 2014;Reed, Bargmann, 2021;Hajeri et al, 2022). Известно, что салат хорошо отзывчив на различные условия культивирования in vitro (Doerschug, Miller, 1967;Michelmore, Eash, 1986;Song et al, 2014). С использованием культуры протопластов получены соматические гибриды между культурным салатом L. sativa и диким видом L. virosa.…”

“…Кроме того, удвоение числа хромосом у полученных гаплоидов спонтанно происходит лишь у части растений. Наибольшая частота спонтанного удвоения хромосом наблюдается у растений B. rapa (Takahashi et al, 2012;Lee et al, 2014), и разновидностей B. oleracea (брокколи, кольраби) (Dias, 2003;Yuan et al, 2015). Повышение частоты регенерации и формирование проростков из эмбриоидов без промежуточных стадий, а также повышение частоты спонтанной диплоидизации могло бы обеспечить производство большего числа дигаплоидов с минимальными усилиями и техническими ресурсами, что в свою очередь облегчило бы создание гибридов F 1 капустных культур.…”

Anti-oviposition and repellence of Cordyceps fumosorosea against Spodoptera exigua

“…The Chinese cabbage (Brassica rapa chinensis L.) is an important commodity in South Korea (Lee et al 2014). This crop ranked third after rice and fresh vegetables in annual production; but nowadays, its demand increases because of its usage in preparing the native dish named Kimchi (Park et al 2014).…”