Tomato (Lycopersicon esculentum Mill.) is an economically important and widely cultivated vegetable crop that is consumed both fresh and processed. The nutritional value of tomato fruits is related to the content of carotenoids, polyphenols, sugars, organic acids, minerals and vitamins. Currently, there is a growing interest in the qualitative and quantitative increase in the content of health-promoting compounds in tomato fruits. VIR Lycopersicon (Tourn.) Mill. genetic resources collection includes 7678 accessions of one cultivated and nine wild species, which in turn provides ample opportunities for searching for information on the variability of the content of biologically active substances and searching for sources with a high content of them in the gene pool. Our work presents the results of the study of 70 accessions of cultivated and wild tomato on the main biochemical characteristics: the content of dry matter, ascorbic acid, sugars, carotenoids, chlorophylls and anthocyanins. As the basis for the selection of accessions for the study, accessions with various colors of fruits, including new accessions with varying content of anthocyanin, were taken. As a result of this study, the amplitude of variability in the content of dry matter (3.72–8.88 and 9.62–11.33 %), sugars (1.50–5.65 and 2.20–2.70 %), ascorbic acid (12.40–35.56 and 23.62– 28.14 mg/100 g), titratable acidity (0.14–0.46 and 0.33–0.48 %), chlorophylls (0.14–5.11 and 2.95–4.57 mg/100 g), carotenoids (0.97–99.86 and 1.03–10.06 mg/100 g) and anthocyanins (3.00–588.86 and 84.31–152.71 mg/100 g) in the fruits of cultivated and wild tomatoes, respectively, was determined. We have determined correlations between the content of dry matter and monosaccharides (r = 0.40, p ≤ 0.05), total sugars (r = 0.37, p ≤ 0.05) and ascorbic acid (r = 0.32, p ≤ 0.05); the content of ascorbic acid and carotenoids (r = 0.25, p ≤ 0.05). A high dependence of the content of chlorophyll a and b among themselves (r = 0.89, p ≤ 0.05), as well as between the content of chlorophyll b and anthocyanins (r = 0.47, p ≤ 0.05), the content of β-carotene (r = 0.26, p ≤ 0.05) and the content of monosaccharides (r = –0.29, p ≤ 0.05) has been noted. We have identif ied tomato accessions with a high content of individual chemical substances, as well as with a complex of traits that can be used as sources in breeding for a high content of dry matter, sugars, ascorbic acid, pigments and anthocyanins.

Small radish and radish are economically important root crops that represent an integral part of a healthy human diet. The world collection of Raphanus L. root crops, maintained in the VIR genebank, includes 2810 accessions from 75 countries around the world, of which 2800 (1600 small radish, 1200 radish) belong to R. sativus species, three to R. raphanistrum, three to R. landra, and four to R. caudatus. It is necessary to systematically investigate the historical and modern gene pool of root-bearing plants of R. sativus and provide new material for breeding. The material for our research was a set of small radish and radish accessions of various ecological groups and different geographical origin, fully covering the diversity of the species. The small radish subset included 149 accessions from 37 countries, belonging to 13 types of seven varieties of European and Chinese subspecies. The radish subset included 129 accessions from 21 countries, belonging to 18 types of 11 varieties of European, Chinese, and Japanese subspecies. As a result of the evaluation of R. sativus accessions according to phenological, morphological, and biochemical analyses, a wide variation of these characteristics was revealed, which is due to the large genetic diversity of small radish and radish of various ecological and geographical origins. The investigation of the degree of variation regarding phenotypic and biochemical traits revealed adaptive stable and highly variable characteristics of R. sativus accessions. Such insights are crucial for the establishment and further use of trait collections. Trait collections facilitate germplasm use and contribute significantly to the preservation of genetic diversity of the gene pool.

Germplasm collections reflect the genetic variability in crops and their wild relatives. Hence, those genetic resources are tremendously valuable for breeders and researchers, especially in light of climatic change and stagnant crop production rates. In order to achieve improvements in crop production and end-use quality, favorable traits and donor alleles present in germplasm collections need to be identified and utilized. This review covers recent reports on the utilization of germplasm material to isolate genotypes of Brassica oleracea and B. rapa vegetables, focusing on high nutrient use efficiency, accumulation of biologically active metabolites, pest resistance, and favorable phenotypic appearance. We discuss the current state of Brassica vegetable collections in genebanks and summarize studies directed to the molecular characterization of those collections.

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The article presents the results of studying the biochemical composition of the large worldwide vegetable crops collection of the Brassicaceae family, stored at the All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg, Russia, consisting of 7303 accessions of different status (approximately 50% unique accessions) from 98 countries. Studied vegetable brassicas collection includes 1 representatives of all botanical varieties, agro-ecological groups and cultivar types of the species Brassica oleracea L. and related wild Mediterranean species, leafy and root vegetable crops B. rapa L., leafy and root vegetable accessions of Indian mustard B. juncea Czern. subsp. integrifolia (H. West) Tell. and subsp. foliosa L. H. Bailey, radish and small radish Raphanus sativus L., garden cress Lepidium sativum L., salad rocket Eruca sativa L.The application of complex analysis for the study of nutrient and biologically active compounds of economically important crops from six species of Brassicaceae family, which determine the quality of vegetables and feeds, also the search within each crop for sources of valuable biochemical traits for breeding use has been presented. The broadest variability of all studied traits between and within crops was revealed, the limits of natural variability were determined.The average values of the studied traits in six species within the family varied to varying degrees: they were similar in the studied species in terms of dry matter content %, protein, total acidity; differed between species to a moderate degree in terms of the content of ascorbic acid, carotene, and the amount of volatile phenolic compounds. The species differed to a very high degree from each other in terms of the average content of sugars (total, monosaccharides, etc.), carotenoids, β-carotene, chlorophylls a and b, anthocyanins, free amino acids, and free fatty acids. Studied crops, belongs toBrassica oleracea, has the highest total sugar content among the all studied crops. B. rapa leafy crops has the highest content of phenolic compounds; B. juncea -β-carotene and chlorophylls; Raphanus sativus -carotenes and anthocyanins; Lepidium sativum -the highest content of protein, total acidity, free amino acids; Eruca sativa -carotenoids and free fatty acids. 2 The maximum dry matter content was detected in the forage turnip B. rapa and Brussels sprout (B. oleracea), protein in leafy B. rapa crops and cauliflower (B. oleracea), total sugars and ascorbic acid in head cabbage (B. oleracea), carotenoids in L. sativum and E. sativa, carotenes, including β-carotene, chlorophylls, phenolic compounds, free fatty acids in B. rapa, anthocyanins in R. sativus, free amino acids in B. rapa and R. sativus.Among the studied variety types of each crop, sources of nutrients and biologically active substances were identified, including types with an optimal biochemical compounds composition for a balanced the human nutrition, which are proposed to be useful in breeding of the new cultivars for healthy diet and medical a...