High-Frequency Induction of Multiple Shoots and Plant Regeneration From Cotyledonary Node Explants of Tongkat Ali (Eurycoma Longifolia Jack)

Alttaher, Annor Gebril Annour; Yusof, Zetty Norhana Balia; Mahmood, Marziah; Shaharuddin, Noor Azmi

doi:10.15666/aeer/1805_63216333

Cited by 5 publications

(4 citation statements)

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“…Thus, in our study, BAP was found to be a superior cytokinin in regenerating shoots than kinetin. Earlier reports also suggested that BAP is a better cytokinin for shoot regeneration in several plants, such as Chenopodium quinoa [ 39 ], Eurycomalongifolia [ 40 ] and Cicer arietinum [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Bansal

Mujib

Siddiqui

et al. 2022

Genes

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Digitalis purpurea L. is a therapeutically important plant that synthesizes important cardiotonics such as digitoxin and digoxin. The present work reports a detailed and efficient propagation protocol for D. purpurea by optimizing various PGR concentrations in Murashige and Skoog (MS) medium. The genetic homogeneity of in vitro regenerants was assessed by the flow cytometric method (FCM) and Start Codon Targeted (SCoT) marker technique. Firstly, the seeds inoculated in full MS medium added with 0.5 mg/L GA3 produced seedlings. Different parts such as hypocotyl, nodes, leaves and apical shoots were used as explants. The compact calli were obtained on BAP alone or in combinations with 2, 4-D/NAA. The hypocotyl-derived callus induced somatic embryos which proliferated and germinated best in 0.75 mg/L BAP-fortified MS medium. Scanning electron microscopic (SEM) images confirmed the presence of various developmental stages of somatic embryos. Shoot regeneration was obtained in which BAP at 1.0 mg/L and 2.0 mg/L BAP + 0.5 mg/L 2,4-D proved to be the best treatments of PGRs in inducing direct and indirect shoot buds. The regenerated shoots showed the highest rooting percentage (87.5%) with 24.7 ± 1.9 numbers of roots/shoot in 1.0 mg/L IBA augmented medium. The rooted plantlets were acclimatized in a greenhouse at a survival rate of 85–90%. The genome size and the 2C nuclear DNA content of field-grown, somatic embryo-regenerated and organogenic-derived plants were estimated and noted to be 3.1, 3.2 and 3.0 picogram (pg), respectively; there is no alteration in ploidy status and the DNA content, validating genetic uniformity. Six SCoT primers unveiled 94.3%–95.13% monomorphic bands across all the plant samples analyzed, further indicating genetic stability among in vitro clones and mother plants. This study describes for the first time successful induction of somatic embryos from hypocotyl callus; and flow cytometry and SCoT marker confirmed the genetic homogeneity of regenerated plants.

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Section: Discussionmentioning

confidence: 99%

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Bansal

Mujib

Siddiqui

et al. 2022

Genes

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“…Each part of the plant is used as a therapeutic agent due to its various medicinal values and the most important part of the plant is the root. 16 Traditionally, the use of TA root extracts as an antiinflammatory and analgesic therapeutic agent is well confirmed. Previous studies had proved that TA root extracts have antioxidant, anti-inflammatory, antipyretic, antimalarial, cytotoxic, aphrodisiac, and antimicrobial properties.…”

Section: Wound Creation and Study Designmentioning

confidence: 99%

“…Previous studies had proved that TA root extracts have antioxidant, anti-inflammatory, antipyretic, antimalarial, cytotoxic, aphrodisiac, and antimicrobial properties. [16][17][18][19][20][21][22][23] In our previous study, ethanol extract of…”

Section: Wound Creation and Study Designmentioning

confidence: 99%

The Effect of The Topical Application of Eurycoma Longifolia Jack (TA) Root Extract Hydrogel on Vascular Endothelial Growth Factor (VEGF) Expression During Wound Healing in vivo Excisional Wound Model

Al-Bayati

Faisal

Fuaat

et al. 2023

imjm

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INTRODUCTION: Medicinal plants are known for their positive impacts on wound healing by stimulating angiogenesis in the skin. Eurycoma longifolia Jack locally known as Tongkat Ali (TA), is a medicinal plant characterised by anti-inflammatory and antioxidant effects. MATERIALS AND METHODS: Excisional wound (15mm × 15mm) in diameter and (2mm) depth was created at the back of 20 male Sprague Dawley rats by incising the marked skin with sterilized surgical scalpel blade then excised the skin by surgical scissors and toothed forceps. The wounded rats were divided into 4 groups, each group contained 5 rats (n=5). Experimental groups were formed as follows: untreated (-ve) control, Hydrocyn® aqua gel (+ve) control, vehicle hydrogel, and TA hydrogel. All the treatments were applied twice daily for 5 days starting on the first day (wounding day). On Day 5 post-wounding, the granulation tissue was harvested from all groups and evaluated by immunohistochemistry assay for Vascular endothelial growth factor (VEGF) expression. RESULTS: VEGF expression in granulation tissue of rat’s skin treated with TA hydrogel and vehicle hydrogel increased significantly compared to that in the untreated (-ve) control group with p-values of 0.040 and 0.029, respectively. Although there was no significant difference in VEGF expression in granulation tissue of rat’s skin treated with the TA group, Hydrocyn® aqua gel (+ve) control, and vehicle hydrogel groups, our study group showed higher expression of VEGF. CONCLUSION: Our study showed that the topical application of TA hydrogel increased the VEGF expression in granulation tissue of rat’s skin, which is an essential growth factor for wound healing. Thus, there is great potential for TA hydrogel to be an effective wound-healing agent for managing cutaneous wounds.

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“…Using nodal segments, cotyledons, and in vitro leaves as explants, shoots were induced on both half-strength and full-strength MS supplemented with 0.5 mg/L BAP and 1.0 mg/L BAP, respectively. Additionally, roots were induced on half-strength MS supplemented with 10 mg/L and 0.5 mg/L IBA and full-strength MS supplemented with 0.5 mg/L IBA [ 53 , 54 , 55 ]. The light conditions used in the above studies were 16L: 8D with an intensity of 35–150 µmol/m 2 /s.…”

Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

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High-Frequency Induction of Multiple Shoots and Plant Regeneration From Cotyledonary Node Explants of Tongkat Ali (Eurycoma Longifolia Jack)

Cited by 5 publications

References 0 publications

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

The Effect of The Topical Application of Eurycoma Longifolia Jack (TA) Root Extract Hydrogel on Vascular Endothelial Growth Factor (VEGF) Expression During Wound Healing in vivo Excisional Wound Model

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

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