High Frequency of Mayaro Virus IgM among Febrile Patients, Central Brazil

Brunini, Sandra; França, Divânia Dias Silva; Silva, Juliana Brasiel; Silva, Leandro Nascimento; Silva, Flúvia Pereira Amorim; Spadoni, Mariana Basso; Rezza, Giovanni

doi:10.3201/eid2306.160929

Cited by 47 publications

(35 citation statements)

References 10 publications

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“…Since then, MAYV infections have been detected in several states [7][8][9] . MAYV is endemic to the Amazon region and Central Brazil, as confirmed by a high incidence of immunoglobulin M (IgM) against MAYV in febrile patients in the Goiás State 10 . Additionally, Terzian et al 11 isolated a genetically distinct Brazilian MAYV lineage in the State of Acre.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Caldas

Ferreira

Freitas

2018

Rev. Soc. Bras. Med. Trop.

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Introduction: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A 1 (PGA 1), a hormone which exhibits antiviral activity against both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) viruses. Further, we examined the effects of inducting the stress protein HSP70 following PGA 1 treatment. Methods: Hep-2 cells infected with MAYV were treated with PGA 1 (0.1-6μg/ml) 12h before infection and for different periods post-infection. Inhibition of viral replication inhibition was analyzed via viral titer determination, whereas the effect of PGA 1 on viral morphogenesis was examined via transmission electron microscopy (TEM). Autoradiography (with 35 S methionine labeling) and western blotting were used to assess the effect of PGA 1 treatment on viral and cellular protein synthesis, and on HSP70 induction, respectively. Results: PGA 1 strongly reduced viral replication in Hep-2 cells, particularly when added during the early stages of viral replication. Although PGA 1 treatment inhibited viral replication by 95% at 24 hours post-infection (hpi), viral structural protein synthesis was inhibited only by 15%. TEM analysis suggested that PGA 1 inhibited replication before viral morphogenesis. Western blot and densitometry analyses showed that PGA 1 treatment increased HSP70 protein levels, although this was not detectable via autoradiography. Conclusions: PGA 1 inhibits MAYV replication in Hep-2 cells at early stages of viral replication, prior to production of viral structural proteins, possibly via HSP70 induction.

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Section: Introductionmentioning

confidence: 99%

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Caldas

Ferreira

Freitas

2018

Rev. Soc. Bras. Med. Trop.

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“…Usually the wrist, ankle, hands, and feet joints are significantly affected, and symptoms may persist for several months, incapacitating the infected person [22,23,29]. In recent years in Brazil, several cases of MAYV have been registered in Pará (2008), Mato Grosso (2012), and Goiás (2014-2016) [20][21][22][30][31][32][33][34]. Although there is still no evidence of the transmission efficiency of MAYV in an urban cycle, it has the potential to establish an epidemic scenario in the Americas, similar to what occurred with ZIKV and CHIKV [35].…”

Section: Introductionmentioning

confidence: 99%

Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

Pereira¹,

Carvalho²,

Mendonça³

et al. 2020

PLoS Negl Trop Dis

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Newly emerging or re-emerging arthropod-borne viruses (arboviruses) are important causes of human morbidity and mortality worldwide. Arboviruses such as Dengue (DENV), Zika (ZIKV), Chikungunya (CHIKV), and West Nile virus (WNV) have undergone extensive geographic expansion in the tropical and subtropical regions of the world. In the Americas the main vectors of DENV, ZIKV, and CHIKV are mosquito species adapted to urban environments, namely Aedes aegypti and Aedes albopictus, whereas the main vector of WNV is Culex quinquefasciatus. Given the widespread distribution in the Americas and high permissiveness to arbovirus infection, these mosquito species may play a key role in the epidemiology of other arboviruses normally associated with sylvatic vectors. Here, we test this hypothesis by determining the vector competence of Ae. aegypti, Ae. albopictus, and Cx. quinquefasciatus to Mayaro (MAYV) virus, a sylvatic arbovirus transmitted mainly by Haemagogus janthinomys that has been causing an increasing number of outbreaks in South America, namely in Brazil. Using field mosquitoes from Brazil, female mosquitoes were experimentally infected, and their competence for infection and transmission rates of MAYV was evaluated. We found consistent infection rate for MAYV in Ae. aegypti (57.5%) and Ae. albopictus (61.6%), whereas very low rates were obtained for Cx. quinquefasciatus (2.5%). Concordantly, we observed high potential transmission ability in Ae. aegypti and Ae. albopictus (69.5% and 71.1% respectively), in contrast to Cx. quinquefasciatus, which could not transmit the MAYV. Notably, we found that very low quantities of virus present in the saliva (undetectable by RT-qPCR) were sufficiently virulent to guarantee transmission. Although Ae. aegypti and Ae. albopictus mosquitoes are not the main vectors for MAYV, our studies suggest that these mosquitoes could play a significant role in the transmission of this arbovirus, since both species showed significant vector competence for MAYV (Genotype D), under laboratory conditions.

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“…Additional reports found MAYV exposure in at least 34% of the subjects at certain study sites (41,42), while VEEV exposure was reported in up to 84% of the populations studied (18). After the recent spread of CHIKV throughout the New World, a study of acute febrile illness in Brazil identified CHIKV antibodies in 5 (6.7%) out of 75 DENV-negative samples and MAYV antibodies were detected in 15 (55.6%) out of 27 DENV-and CHIKV-negative samples (43). Misdiagnosis, especially in countries where DENV is endemic, can be problematic.…”

Section: Discussionmentioning

confidence: 97%

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

Smith

Pugh

Cisney

et al. 2018

mSphere

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Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), and chikungunya virus (CHIKV) are vector-borne alphaviruses that cocirculate in South America. Human infections by these viruses are frequently underdiagnosed or misdiagnosed, especially in areas with high dengue virus endemicity. Disease may progress to debilitating arthralgia (MAYV, CHIKV), encephalitis (VEEV), and death. Few standardized serological assays exist for specific human alphavirus infection detection, and antigen cross-reactivity can be problematic. Therefore, serological platforms that aid in the specific detection of multiple alphavirus infections will greatly expand disease surveillance for these emerging infections. In this study, serum samples from South American patients with PCR-and/or isolation-confirmed infections caused by MAYV, VEEV, and CHIKV were examined by using a protein microarray assembled with recombinant capsid, envelope protein 1 (E1), and E2 from nine New and Old World alphaviruses. Notably, specific antibody recognition of E1 was observed only with MAYV infections, whereas E2 was specifically targeted by antibodies from all of the alphavirus infections investigated, with evidence of cross-reactivity to E2 of o'nyong-nyong virus only in CHIKV-infected patient serum samples. Our findings suggest that alphavirus structural protein microarrays can distinguish infections caused by MAYV, VEEV, and CHIKV and that this multiplexed serological platform could be useful for high-throughput disease surveillance.IMPORTANCE Mayaro, chikungunya, and Venezuelan equine encephalitis viruses are closely related alphaviruses that are spread by mosquitos, causing diseases that produce similar influenza-like symptoms or more severe illnesses. Moreover, alphavirus infection symptoms can be similar to those of dengue or Zika disease, leading to underreporting of cases and potential misdiagnoses. New methods that can be used to detect antibody responses to multiple alphaviruses within the same assay would greatly aid disease surveillance efforts. However, possible antibody cross-reactivity between viruses can reduce the quality of laboratory results. Our results demonstrate that antibody responses to multiple alphaviruses can be specifically quantified within the same assay by using selected recombinant protein antigens and further show that Mayaro virus infections result in unique responses to viral envelope proteins.

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High Frequency of Mayaro Virus IgM among Febrile Patients, Central Brazil

Cited by 47 publications

References 10 publications

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages

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