High frequency production of embryos from liquid flask cultures of oilseed rape

Loh, Chiang‐Shiong; Shu, W.; Khor, Eugene

doi:10.1002/(sici)1097-0290(19970505)54:3<231::aid-bit4>3.0.co;2-g

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…The embryogenic masses subcultured with maltose and KIN, and transferred from dark to light conditions, resulted in some shoot meristem development from embryos. It is also known that KIN can induce somatic embryogenesis in both woody and herbaceous species, such as Sorghum bicolor (L.) Moench (Gendy et al, 1996), Citropsis schweinfurthii (Jumin and Nito, 1996), Brassica napus (Loh et al, 1997) and Prunus avium L. (Garin et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…The choice of medium components can affect development of specific embryogenic stages and their conversion into plants; however, auxins and cytokinins are always required for starting the embryogenic process. In addition to the plant growth regulators most commonly used (BAP,NAA,2,4, for initiating embryogenisis, other synthetic cytokinins such as thiazuron stimulate not only somatic embryogenesis but also shoot differentiation in woody species such as white ash (Bates et al, 1992) and grapevine (Martinelli et al, 1991); also kinetin can induce somatic embryogenesis in both woody and herbaceous species, such as Sorghum bicolor (L.) Moench (Gendy et al, 1996), Citropsis schweinfurthii (Jumin and Nito, 1996), Brassica napus (Loh et al, 1997) and Prunus avium L. (Garin et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Influence of plant growth regulators, carbon sources and iron on the cyclic secondary somatic embryogenesis and plant regeneration of transgenic cherry rootstock `Colt' (Prunus avium � P. pseudocerasus)

Pesce

Rugini

2004

Plant Cell, Tissue and Organ Culture

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The frequency of long-term secondary somatic embryogenesis and shoot meristem development from embryogenic masses of the cherry rootstock 'Colt' (Prunus avium · P. pseudocerasus), differentiated from transgenic roots containing the T-DNA of Agrobacterium rhizogenes, has opened the way for genetic improvement by biotechnological techniques. Whole plants were produced by stimulating shoot meristem development from somatic embryos. The combination of 4 mg l )1 of kinetin and 2% of maltose under illumination stimulated shoot development and, subsequently, whole plants have been recovered by applying 1.5 mg l )1 kinetin to the rooting medium. Although numerous treatments have been tested involving both embryogenic masses and whole embryos, normal embryo germination was observed sporadically. Cold treatment was effective in stimulating secondary somatic embryogenesis with embryo development to the cotyledonary stage, but did not promote their germination. Similarly, a higher concentration (44-55 mg l )1 ) of chelated iron than that commonly used in tissue culture media (36.7 mg l )1 ) produced, after 3 weeks in culture, almost a 50% increase in the number of embryos at the cotyledonary stage per embryogenic mass. Among the cytokinins tested, 1 mg l )1 of 6-benzylaminopurine and 0.1 mg l )1 of thidiazuron were effective in inducing secondary somatic embryogenesis; however, each of them expressed highest efficiency with specific medium and environmental conditions. Furthermore, application of 1 mg l )1 thidiazuron reverted morphogenic callus to non-morphogenic callus, particularly in medium containing 2% sucrose. Finally, hormone free medium with 2% maltose enhanced maturation of the embryos to the normal cotyledonary stage. This paper has improved knowledge of embryo culture and plant production in this important genotype, opening the way for genetic improvement by biotechnological techniques, mainly with the aim of modifying the growth pattern of the canopy of sweet cherry grafted on it.Abbreviations: BAP -6-benzylaminopurine; BM -basal medium; 2,4D D -2,4-dichlorophenoxyacetic acid; GA 3 -gibberellic acid; KIN -kinetin; MS -Murashige and Skoog; NAA -naphthalenacetic acid; PGRplant growth regulators; SSE -secondary somatic embryogenesis; TDZ -thidiazuron

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of plant growth regulators, carbon sources and iron on the cyclic secondary somatic embryogenesis and plant regeneration of transgenic cherry rootstock `Colt' (Prunus avium � P. pseudocerasus)

Pesce

Rugini

2004

Plant Cell, Tissue and Organ Culture

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Regeneration of Plants via Somatic Embryogenesis

2004

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Production of somatic embryos and shoots from sugarbeet callus: Effects of abscisic acid, other growth regulators, nitrogen source, sucrose concentration and genotype

Saunders

Tsai

1999

In Vitro Cell.Dev.Biol.-Plant

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Two sugarbeet (Beta vulgaris L.) genotypes, REL-1 and REL-2, were used to measure the level of somatic embryo and shoot production fiom hormone-autonomous callus plated under varied nutrient medium combinations of abscisic acid with the growth regulators 6-benzyladenine, 1-naphthaleneacetic acid, or 2,4-dichlorophenoxyacetic acid, with eight sole nitrogen sources, or with different sucrose concentrations. Clone REL-2 produced embryos up to 35-fold more frequently than clone REL-1. Inclusion of abscisic acid at some concentrations consistently improved embryo production in all experiments and was observed to stimulate shoot production. At some concentrations, 1-naphthaleneacetic acid as well as urea and glutamine stimulated greater embryo production over the control, but only for REL-1, for which there was greater room for improvement. Three and five percent sucrose were superior to 1, 7, and 9%. Higher initial 6-benzyladenine concentration [in the range 0, 0.1-1.0 mg/L (0.44M,.44 gM)] was associated with lower embryo production but greater shoot regeneration for both clones. REL-2 was significantly better than REL-1 in shoot regeneration. The range of embryo production was more than 35-fold between genotypes, whereas the range of physiological effects was no greater than 10-fold. REL-2 has been released to sugarbeet researchers because of its superior embryogenic and shoot regeneration abilities for application in biotechnology.

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High frequency production of embryos from liquid flask cultures of oilseed rape

Cited by 4 publications

References 13 publications

Influence of plant growth regulators, carbon sources and iron on the cyclic secondary somatic embryogenesis and plant regeneration of transgenic cherry rootstock `Colt' (Prunus avium � P. pseudocerasus)

Influence of plant growth regulators, carbon sources and iron on the cyclic secondary somatic embryogenesis and plant regeneration of transgenic cherry rootstock `Colt' (Prunus avium � P. pseudocerasus)

Regeneration of Plants via Somatic Embryogenesis

Production of somatic embryos and shoots from sugarbeet callus: Effects of abscisic acid, other growth regulators, nitrogen source, sucrose concentration and genotype

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