High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China

Manawasinghe, Ishara S.; Dissanayake, Asha J.; Li, Xinghong; Liu, Mei; Wanasinghe, Dhanushka N.; Xu, Jianping; Zhao, Wensheng; Zhang, Wei; Zhou, Ying; Hyde, Kevin D.; Brooks, Siraprapa; Yan, Jiye

doi:10.3389/fmicb.2019.01936

Cited by 85 publications

(79 citation statements)

References 95 publications

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“…Sequence files were processed using the pattern analysis software package BioEdit Sequence Alignment Editor v.7.0.5.0 (Hall, 1999). To establish the identity of the strains at the species level, combined phylogenetic analyses of the three considered loci (ITS + tub + EF1-α) were conducted against those of different species sequences selected by extensive literature review (Udayanga et al, 2011(Udayanga et al, , 2014a(Udayanga et al, ,b, 2015Gomes et al, 2013;Lombard et al, 2014;Huang et al, 2015;Dissanayake et al, 2017a,b;Gao et al, 2017;Santos et al, 2017a,b;Fan et al, 2018;Guarnaccia et al, 2018;Yang et al, 2018;Long et al, 2019;Manawasinghe et al, 2019;Marin-Felix et al, 2019;Ozawa et al, 2019;Zhou and Hou, 2019;Hilário et al, 2020) and retrieved from the National Center for Biotechnology Information (NCBI) database ( Supplementary Table 1). Individual alignments were performed using the MUSCLE tool (Edgar, 2004) implemented in MegaX (Kumar et al, 2018).…”

Section: Molecular Data Analysismentioning

confidence: 99%

Molecular Characterization of Diaporthe Species Associated With Hazelnut Defects

et al. 2020

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Fungi of the genus Diaporthe have been reported as the main causative agent of hazelnut defects in the Caucasus area. This study aimed to define which fungal species are present in defective hazelnuts grown in Turkey and confirm the role of Diaporthe spp. Seven hazelnut orchards were selected, with each one located in a different Turkish Province (Düzce, Giresun, Ordu, Samsun, Sakarya, Trabzon, and Zonguldak), and hazelnuts were collected at early and full ripening. Fungal isolation and identification were performed at the genus level based on morphological characteristics. Several genera were isolated, with Diaporthe spp. being among the prevalent. This was the only genus with increasing incidence from early to full ripening, and incidence at full ripening was positively correlated both with internal (ρ = 0.86) and visible defects (ρ = 0.81), which confirmed its role as the key causative agent of hazelnut defects. The correlation of defect occurrence with rainfall, reported in previous study, was not confirmed, possibly due to the low defect incidence. A total of 86 Diaporthe monosporic strains isolated from Turkish hazelnut samples, together with 33 strains collected in the Caucasus region and 6 from Italy, were analyzed with a multi-locus phylogeny based on three genomic loci (ITS, EF1-α, and tub). The results showed that Diaporthe strains can be grouped into 7 distinct clades, with a majority of Turkish strains (95%) being placed into a single clade related with D. eres. These samples were organized into several sub-clades, which indicates the existence of genetically diverse sub-populations.

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Section: Molecular Data Analysismentioning

confidence: 99%

Molecular Characterization of Diaporthe Species Associated With Hazelnut Defects

et al. 2020

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“…However, with the development of molecular methods, species identification based on multigene phylogeny together with morphological characters provided a more accurate identification ( Udayanga et al, 2011 ; Gao et al, 2017 ). In recent studies, ITS, tef1 , tub2 , cal , and partial histone ( HIS ) genes have been used for species delimitation in this genus ( Udayanga et al, 2014a ; Santos et al, 2017 ; Guarnaccia and Crous, 2018 ; Manawasinghe et al, 2019 ). Diaporthe species have a worldwide distribution and diverse host associations ( Udayanga et al, 2014b ).…”

Section: Introductionmentioning

confidence: 99%

Endophytic Diaporthe Associated With Citrus grandis cv. Tomentosa in China

et al. 2021

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Diaporthe species are associated with Citrus as endophytes, pathogens, and saprobes worldwide. However, little is known about Diaporthe as endophytes in Citrus grandis in China. In this study, 24 endophytic Diaporthe isolates were obtained from cultivated C. grandis cv. “Tomentosa” in Huazhou, Guangdong Province in 2019. The nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-α (tef1), β-tubulin (tub2), and partial calmodulin (cal) gene regions were sequenced and employed to construct phylogenetic trees. Based on morphology and combined multigene phylogeny, eleven Diaporthe species were identified including two new species, Diaporthe endocitricola and D. guangdongensis. These are the first report of D. apiculata, D. aquatica, D. arecae, D. biconispora, D. limonicola, D. masirevicii, D. passifloricola, D. perseae, and D. sennae on C. grandis. This study provides the first intensive study of endophytic Diaporthe species on C. grandis cv. tomentosa in China. These results will improve the current knowledge of Diaporthe species associated with C. grandis. The results obtained in this study will also help to understand the potential pathogens and biocontrol agents and to develop a platform in disease management.

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“…Dissanayake et al [45] provided accounts for 171 accepted species with a backbone tree for the genus. Manawasinghe et al [46] contributed to the understanding of this genus by studying the high genetic diversity and species complexity of Diaporthe associated with grapevine dieback.…”

Section: Plant Pathogensmentioning

confidence: 99%

The rise of mycology in Asia

Hyde¹,

Chethana²,

Jayawardena³

et al. 2020

ScienceAsia

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Mycology was a well-studied discipline in Australia and New Zealand, Europe, South Africa and the USA. In Asia (with the exception of Japan) and South America, the fungi were generally poorly known and studied, except for the result of forays from some American and European mycologists. However, in the last 20 years, the situation has changed. With the development of Asian economies, the funding for science research and development has greatly increased. Mycological research has also diversified in many fields. Many studies have focused on applied aspects and new journals and websites have been established as a platform for Asian mycologists to publish their research. This paper will briefly review the history of the study of fungi in Asia and then discuss how it advanced during the last two decades. It will examine the current situation using case studies in plant pathogens, terrestrial saprobes, aquatic fungi, evolution studies, genomics and applied mycology and biotechnology. Finally, it will suggest research that is needed in the future.

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High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China

Cited by 85 publications

References 95 publications

Molecular Characterization of Diaporthe Species Associated With Hazelnut Defects

Molecular Characterization of Diaporthe Species Associated With Hazelnut Defects

Endophytic Diaporthe Associated With Citrus grandis cv. Tomentosa in China

The rise of mycology in Asia

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