High glucosinolate broccoli: a delivery system for sulforaphane

Sarıkamış, Gölge; Marquez, Julietta; MacCormack, R.; Bennett, Richard N.; Roberts, Jeremy A.; Mithen, Richard

doi:10.1007/s11032-006-9029-y

Cited by 67 publications

(41 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The underlying genes are likely to correspond directly to cloned genes that have been functionally analysed in Arabidopsis, described above. In addition, quantitative trait loci (QTLs) have been identified that determine the overall level of GLSs in both B. napus [59,60] and B. oleracea [61,62]. The genetic regulation of indolyl glucoisnolates derived from tryptophan is far less advanced.…”

Section: Breeding For Altered Gls Content In Brassicamentioning

confidence: 99%

Glucosinolates inBrassicavegetables: The influence of the food supply chain on intake, bioavailability and human health

Verkerk¹,

Schreiner

Krumbein

et al. 2009

Molecular Nutrition Food Res

Self Cite

526

424

View full text Add to dashboard Cite

Glucosinolates (GLSs) are found in Brassica vegetables. Examples of these sources include cabbage, Brussels sprouts, broccoli, cauliflower and various root vegetables (e.g. radish and turnip). A number of epidemiological studies have identified an inverse association between consumption of these vegetables and the risk of colon and rectal cancer. Animal studies have shown changes in enzyme activities and DNA damage resulting from consumption of Brassica vegetables or isothiocyanates, the breakdown products (BDP) of GLSs in the body. Mechanistic studies have begun to identify the ways in which the compounds may exert their protective action but the relevance of these studies to protective effects in the human alimentary tract is as yet unproven. In vitro studies with a number of specific isothiocyanates have suggested mechanisms that might be the basis of their chemoprotective effects. The concentration and composition of the GLSs in different plants, but also within a plant (e.g. in the seeds, roots or leaves), can vary greatly and also changes during plant development. Furthermore, the effects of various factors in the supply chain of Brassica vegetables including breeding, cultivation, storage and processing on intake and bioavailability of GLSs are extensively discussed in this paper.

show abstract

Section: Breeding For Altered Gls Content In Brassicamentioning

confidence: 99%

Glucosinolates inBrassicavegetables: The influence of the food supply chain on intake, bioavailability and human health

Verkerk¹,

Schreiner

Krumbein

et al. 2009

Molecular Nutrition Food Res

Self Cite

526

424

View full text Add to dashboard Cite

show abstract

“…Different members of this family may catalyze different number of rounds of elongation, with genetic variation at the GS-ELONG and GS-PRO loci enabling selection for different glucosinolate profiles, while allelic variation at the quantitative trait loci determines overall amount (286). These initiating factors have been observed in both Arabidopsis and Brassica (58,242). In B. oleracea, these genes determine the length of the side chain with either one or two rounds of methionine elongation to produce 3C or 4C glucosinolates, respectively (242).…”

Section: Introductionmentioning

confidence: 99%

Dietary Sulforaphane in Cancer Chemoprevention: The Role of Epigenetic Regulation and HDAC Inhibition

Tortorella

Royce²,

Licciardi

et al. 2015

Antioxidants & Redox Signaling

189

110

View full text Add to dashboard Cite

Significance: Sulforaphane, produced by the hydrolytic conversion of glucoraphanin after ingestion of cruciferous vegetables, particularly broccoli and broccoli sprouts, has been extensively studied due to its apparent health-promoting properties in disease and limited toxicity in normal tissue. Recent Studies: Recent identification of a sub-population of tumor cells with stem cell-like self-renewal capacity that may be responsible for relapse, metastasis, and resistance, as a potential target of the dietary compound, may be an important aspect of sulforaphane chemoprevention. Evidence also suggests that sulforaphane may target the epigenetic alterations observed in specific cancers, reversing aberrant changes in gene transcription through mechanisms of histone deacetylase inhibition, global demethylation, and microRNA modulation. Critical Issues: In this review, we discuss the biochemical and biological properties of sulforaphane with a particular emphasis on the anticancer properties of the dietary compound. Sulforaphane possesses the capacity to intervene in multistage carcinogenesis through the modulation and/or regulation of important cellular mechanisms. The inhibition of phase I enzymes that are responsible for the activation of pro-carcinogens, and the induction of phase II enzymes that are critical in mutagen elimination are well-characterized chemopreventive properties. Furthermore, sulforaphane mediates a number of anticancer pathways, including the activation of apoptosis, induction of cell cycle arrest, and inhibition of NFjB. Future Directions: Further characterization of the chemopreventive properties of sulforaphane and its capacity to be selectively toxic to malignant cells are warranted to potentially establish the clinical utility of the dietary compound as an anticancer compound alone, and in combination with clinically relevant therapeutic and management strategies.

show abstract

“…If the glucosinolate side chain contains a double bond (alkene) in the chemical structure, in the presence of epithiospecifer protein (ESP) and ferrous ions, the thiohydroximate rearranges to produce an ETN and nitrile (Bones and Rossiter 2006). ESP is more sensitive to thermal processing than myrosinase and short periods of steaming can alter degradation profiles to increase ITCs with a marked reduction in nitriles and ETNs (Sarikamis et al 2006). Thus ETNs and nitriles are more likely to be formed in raw vegetables (Abd Kadir 2013;Kyung et al 1995) such as in salads where for example cabbage is used.…”

Section: Introductionmentioning

confidence: 99%

The selective cytotoxicity of the alkenyl glucosinolate hydrolysis products and their presence in Brassica vegetables.

et al. 2015

View full text Add to dashboard Cite

Cruciferous vegetable consumption correlates with reduced risk of cancer. This chemopreventative activity may involve glucosinolates and their hydrolysis products.Glucosinolate-derived isothiocyanates have been studied for their toxicity and chemopreventative properties, but other hydrolysis products (epithionitriles, nitriles) have not been thoroughly examined. We report that these hydrolysis products differ in their cytotoxicity to human cells, with toxicity most strongly associated with isothiocyanates rather than epithionitriles and nitriles. We explored mechanisms of this differential cytotoxicity by examining the role of oxidative metabolism, oxidative stress, mitochondrial permeability, reduced glutathione levels, cell cycle arrest and apoptosis.2-Propenylisothiocyanate and 3-butenylisothiocyanate both inhibited cytochome P450 1A (CYP1A) enzyme activity in CYP expressing MCL-5 cells at high cytotoxic doses.Incubation of MCL-5 cells with non-cytotoxic doses of 2-propenylisothiocyanate for 24 h resulted in a dose-dependent inhibition of ethoxyresorufin O-deethylase, yet failed to affect CYP1A1 mRNA expression indicating interference with enzyme activity rather than inhibition of transcription. Increased reactive oxygen species (ROS) production was observed only for 2-propenylisothiocyanate treatment. 2-Propenylisothiocyanate treatment lowered reduced glutathione levels whereas no changes were noted with 3,4-epithiobutylnitrile. Cell cycle analysis showed that 2-propenylisothiocyanate induced a G2/M block whereas other hydrolysis products showed only marginal effects. We found that 2-propenylisothiocyanate and 3-butenylisothiocyanate induced cell death predominantly via necrosis whereas, 3,4-epithiobutylnitrile promoted both necrosis and apoptosis. Thus the activity of glucosinolate hydrolysis products includes cytotoxicity that is compound-class specific and may contribute to their putative chemoprotection properties.

show abstract

High glucosinolate broccoli: a delivery system for sulforaphane

Cited by 67 publications

References 29 publications

Glucosinolates inBrassicavegetables: The influence of the food supply chain on intake, bioavailability and human health

Glucosinolates inBrassicavegetables: The influence of the food supply chain on intake, bioavailability and human health

Dietary Sulforaphane in Cancer Chemoprevention: The Role of Epigenetic Regulation and HDAC Inhibition

The selective cytotoxicity of the alkenyl glucosinolate hydrolysis products and their presence in Brassica vegetables.

Contact Info

Product

Resources

About