High hydrostatic pressure inactivation of<i>Lactobacillus plantarum</i>cells in (O/W)-emulsions is independent from cell surface hydrophobicity and lipid phase parameters

Kafka, Thomas A.; Reitermayer, Dominik; Lenz, Christian A.; Vogel, Rudi F.

doi:10.1080/08957959.2017.1347926

Cited by 5 publications

(1 citation statement)

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“…Previously obtained data from our group shows that the presence of fat per se during HHP treatment does not protect L. plantarum cells against HHP-mediated inactivation [ 16 ]. However, it is known that many food environments can protect microorganisms from efficient HHP inactivation, and the effect of pre-conditioning in fat- or fatty acid-containing environments before HHP treatments has not been investigated so far.…”

Section: Introductionmentioning

confidence: 99%

Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

et al. 2018

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Tween® 80 is a frequently used supplement of media for the cultivation of lactic acid bacteria. We investigated its effect on the cell physiology and stress tolerance of Lactobacillus (L.) plantarum. Data on the transcriptomic response to Tween 80 supplementation and its effects on cellular fatty acid profiles and growth characteristics are compared with data characterizing the effect of Tween 80, other Tween types and free fatty acids on the high hydrostatic pressure (HHP) tolerance of L. plantarum strain TMW 1.708. These include effects on cell viability, sub-lethal injury, metabolic activity, protein release and propidium iodide uptake. Tween 80 caused the downregulation of fatty acid biosynthesis and an increase in oleic acid and cyclopropane fatty acid levels in the cell membrane. Tween 20, Tween 80 and free oleic acid, but not Tween 40, Tween 60 and other free fatty acids, conferred resistance against HHP. Tween 80 diminished pressure-induced loss of metabolic activity, protein release and uptake of propidium iodide. However, loss of cell viability exceeded by far membrane permeabilization, suggesting that membrane permeabilization, which has frequently been postulated as a major factor in HHP inactivation of microbes, is not necessarily required for HHP-induced cell death of Lactobacillus plantarum.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1203-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%