High Incidence of Antibodies to HTLV-I tax in Blood Relatives of Adult T Cell Leukemia Patients

Okayama, Akira; Chen, Yi-Ming A.; Thchibana, Nobuyoshi; Shioiri, Shigemasa; Lee, Tun‐Hou; Tsuda, Kazunori; Essex, Max

doi:10.1093/infdis/163.1.47

Cited by 24 publications

(14 citation statements)

References 24 publications

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“…Our findings of HTLV-I pX message by RT-PCR in ATL samples are in agreement with those of Kinoshita et al (35), while contradictory results exist (36). In support of pX gene expression in vivo, anti-tax antibodies are frequently found in ATL patients and in healthy carriers (37).…”

Section: Resultssupporting

confidence: 92%

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

Berneman

Gartenhaus

Reitz

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

143

120

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Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the progression to and maintenance of the leukemic state in vivo is unclear because of the inability of most previous studies to readily detect HTLV-I RNA in infected individuals. By using the reverse transcriptase-polymerase chain reaction, we detected spliced messages for the HTLV-I pX regulatory genes in primary uncultured cells from ATL patients and healthy asymptomatic carriers. In addition to the expected doubly spliced pX message, three alternatively spliced mRNAs were demonstrated (pXA17, pX-p21rex, and pX-orflI mRNAs, where orf = open reading frame). The same splice sites were shown in the messages from uncultured ATL cells and from the HTLV-I-producing C1O/MJ cell line. Alternatively spliced pX mRNAs have the potential to code for known and putative pX gene products. Among the transcripts is a monocistronic mRNA likely to code for p2l'rx (pX-p2lrx mRNA). Since alternative splicing of HTLV

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Section: Resultssupporting

confidence: 92%

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

Berneman

Gartenhaus

Reitz

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

143

120

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“…Singapore). OF samples were also analyzed by western blot with a 50-kDa HTLV-1-tax recombinant protein (21).…”

Section: Spasticmentioning

confidence: 99%

Human T-Lymphotropic virus (HTLV) type I in vivo integration in oral keratinocytes

Domínguez¹,

González.²,

Sánchez³

et al. 2011

Braz. J. Microbiol.

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“…In a 5-year prospective follow-up study conducted in Miyazaki, Japan, a total of five seroconverters have been identified among married couples who were anti-HTLV-I antibody-discordant (26). Since sexual contact with an infected spouse was the most likely source of exposure, we used a recombinant Tax protein (27) ITo whom reprint requests should be addressed.…”

mentioning

confidence: 99%

Sexual transmission of human T-cell leukemia virus type I associated with the presence of anti-Tax antibody.

Chen

Okayama

Lee

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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The tax gene product (Tax protein) of human T-cell leukemia virus type I (EHTLV-I) is a specific transcriptional activator of the viral long terminal repeat sequence and is essential for the replication cycle of the virus. To elucidate the relationship between the presence of anti-Tax antibody and the transmission of the viral infection, annual consecutive serum samples from married couples serologically discordant or concordant for HTLV-I were examined. These included 5 individuals whose spouses seroconverted during this 5-year follow-up study period. The samples were tested by a Western blot assay using a recombinant Tax protein as the antigen. The results showed that 24 of 32 (75%) men in the concordant couples (both husband and wife were HTLV-I carriers) had anti-Tax antibody, while only 5 of 18 (27.8%) men in the discordant couples (husband was carrier and wife was seronegative to HTLV-I) were positive for anti-Tax antibody (P = 0.0012). Furthermore, all spouses of the 5 seroconverters (4 women and 1 man) had anti-Tax antibody, while only 23 of 46 (50%) age-matched randomly selected HTLV-I carriers from the discordant-couple group had anti-Tax antibody. When the data were analyzed by gender, all husbands of the female seroconverters had anti-Tax antibodies, which was signiflcantly higher than the prevalence of anti-Tax antibodies in men who did not transmit the virus to their spouses during the follow-up period (P = 0.017). In addition, antibody reactivity to other HTLV-I antigens (including Env gp46, transmembrane protein gp21, and Gag p19 and p24) were examined. The results indicated no significant difference between the prevalence of antibody reactivity to any of the antigens in the spouses of the seroconverters and the reference group. We conclude that the presence of anti-Tax antibody in men may indicate a high risk of viral transmission to their wives via heterosexual routes.

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High Incidence of Antibodies to HTLV-I tax in Blood Relatives of Adult T Cell Leukemia Patients

Cited by 24 publications

References 24 publications

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

Human T-Lymphotropic virus (HTLV) type I in vivo integration in oral keratinocytes

Sexual transmission of human T-cell leukemia virus type I associated with the presence of anti-Tax antibody.

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