High-intensity vector signals for detecting SARS-CoV-2 RNA using CRISPR/Cas13a couple with stabilized graphene field-effect transistor

Sun, Yang; Yang, Cheng; Jia, Xiaolin; Chen, Shuo; Su, Fengxia; Wang, Hui; Liu, Weiliang; He, Xiaofei; Chen, Lei; Man, Baoyuan; Li, Zhengping

doi:10.1016/j.bios.2022.114979

Cited by 17 publications

(11 citation statements)

References 64 publications

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“…[86] Research progress has also been demonstrated in the rapid nucleic acid detection of unamplified samples. [87][88][89][90] In 2019, Hajian et al combined GFETs with clustered regularly interspaced short palindromic repeat (CRISPR)-associated nuclease (Cas)based technologies to enable the digital detection of a target gene sequence. [76] The as-prepared platform achieved 15-minute detection of unamplified DNA samples with a sensitivity of 1.7 × 10 −15 m. [76] Shortly after, the same research group modified the GFET sensor by using different Cas9 variants and orthologues to improve single-nucleotide polymorphisms (SNP) discrimination, to fabricate a single-nucleotide polymorphism chip and to enable multiplex electrical measurements at the same time (Termed CRISPR-SNP-Chip, Figure 4c).…”

Section: Nucleic Acidmentioning

confidence: 99%

Graphene Transistors for In Vitro Detection of Health Biomarkers

Dai

Kong

Chen

et al. 2023

Adv Funct Materials

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Biomarkers are primary indicators for precise diagnosis and treatment. The early identification of health biomarkers has been sustained by the evolutionary success in sensor technologies. Among them, graphene field-effect transistor (GFET) biosensors have exhibited major advantages such as an ultrashort response time, high sensitivity, easy operation, capability of integration, and label-free detection. Owing to the atomic thickness, graphene restricts charge carrier flow merely at the material surface and responds to foreign stimuli directly, leading to effective signal acquisition and transmission. Here, this review summarizes the latest advances in GFET biosensors in a comprehensive manner that contains the device design, working principle, surface functionalization, and proof-of-concept applications. It provides a comprehensive survey of GFET biosensors with regard to biomarker analysis at the single-device level and integrated prototypes that include wearable sensors, biomimetic systems, healthcare electronics, and diagnostic platforms. Moreover, there is discussion on the long-standing research efforts and outlook for the future development of GFET sensor systems from lab to fab.

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Section: Nucleic Acidmentioning

confidence: 99%

Graphene Transistors for In Vitro Detection of Health Biomarkers

Dai

Kong

Chen

et al. 2023

Adv Funct Materials

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“…The high sensitivity of CRISPR/Cas-based detection is typically based on isothermal amplification that can amplify nucleic acid molecules; however, the use of the isothermal amplification reagents raises the cost of CRISPR/Cas-based diagnosis. Because of its high sensitivity, electrical signal sensing could be a candidate for amplification-free CRISPR/Cas-based diagnosis [ 32 , 33 ]. Electrical signal-based CRISPR/Cas diagnosis is typically performed using a defective Cas protein such as dCas9, which can only bind and not cleave target nucleic acid molecules.…”

Section: Sensing Methods For Crispr/cas-based Detectionmentioning

confidence: 99%

Towards Point of Care CRISPR-Based Diagnostics: From Method to Device

Chen

Zhou

Wang

et al. 2023

JFB

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Rapid, accurate, and portable on-site detection is critical in the face of public health emergencies. Infectious disease control and public health emergency policymaking can both be aided by effective and trustworthy point of care tests (POCT). A very promising POCT method appears to be the clustered regularly interspaced short palindromic repeats and associated protein (CRISPR/Cas)-based molecular diagnosis. For on-site detection, CRISPR/Cas-based detection can be combined with multiple signal sensing methods and integrated into smart devices. In this review, sensing methods for CRISPR/Cas-based diagnostics are introduced and the advanced strategies and recent advances in CRISPR/Cas-based POCT are reviewed. Finally, the future perspectives of CRISPR and POCT are summarized and prospected.

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“…Its biocompatibility can also immobilize biological probes. Typical nano-materials, such as graphene, carbon nano-tubes (CNTs), MoS 2 , and silicon nano-wires (SiNWs), are commonly used as modification materials for the FET sensing layer [ 59 , 60 , 61 , 62 , 63 , 64 ].…”

Section: Applications Of Transistor-based Biosensorsmentioning

confidence: 99%

Applications of Transistor-Based Biochemical Sensors

Gao

Ming

2023

Biosensors

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Transistor-based biochemical sensors feature easy integration with electronic circuits and non-invasive real-time detection. They have been widely used in intelligent wearable devices, electronic skins, and biological analyses and have shown broad application prospects in intelligent medical detection. Field-effect transistor (FET) sensors have high sensitivity, reasonable specificity, rapid response, and portability and provide unique signal amplification during biochemical detection. Organic field-effect transistor (OFET) sensors are lightweight, flexible, foldable, and biocompatible with wearable devices. Organic electrochemical transistor (OECT) sensors convert biological signals in body fluids into electrical signals for artificial intelligence analysis. In addition to biochemical markers in body fluids, electrophysiology indicators such as electrocardiogram (ECG) signals and body temperature can also cause changes in the current or voltage of transistor-based biochemical sensors. When modified with sensitive substances, sensors can detect specific analytes, improve sensitivity, broaden the detection range, and reduce the limit of detection (LoD). In this review, we introduce three kinds of transistor-based biochemical sensors: FET, OFET, and OECT. We also discuss the fabrication processes for transistor sources, drains, and gates. Furthermore, we demonstrated three sensor types for body fluid biomarkers, electrophysiology signals, and development trends. Transistor-based biochemical sensors exhibit excellent potential in multi-mode intelligent analysis and are good candidates for the next generation of intelligent point-of-care testing (iPOCT).

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High-intensity vector signals for detecting SARS-CoV-2 RNA using CRISPR/Cas13a couple with stabilized graphene field-effect transistor

Cited by 17 publications

References 64 publications

Graphene Transistors for In Vitro Detection of Health Biomarkers

Graphene Transistors for In Vitro Detection of Health Biomarkers

Towards Point of Care CRISPR-Based Diagnostics: From Method to Device

Applications of Transistor-Based Biochemical Sensors

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