2000
DOI: 10.1098/rspb.2000.1157
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High intraindividual variation in internal transcibed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics

Abstract: Aeschynanthus (Gesneriaceae) is a large genus of tropical epiphytes that is widely distributed from the Himalayas and China throughout South-East Asia to New Guinea and the Solomon Islands. Polymerase chain reaction (PCR) consensus sequences of the internal transcribed spacers (ITS) of Aeschynanthus nuclear ribosomal DNA showed sequence polymorphism that was difficult to interpret. Cloning individual sequences from the PCR product generated a phylogenetic tree of 23 Aeschynanthus species (two clones per specie… Show more

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Cited by 55 publications
(35 citation statements)
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“…However, several species could not be discriminated from each other by either ITS or matK regions. On the other hand, the ITS region requires cloning before sequencing because of the allelic polymorphisms (presence of insertion/deletion, single nucleotide polymorphism), pseudogenes, and paralogous copies of the ITS region in a plant species (Buckler et al, 1997;Denduangboripant and Cronk, 2000;Kita and Ito, 2000;Bailey et al, 2003;Li et al, 2004;Razafimandimbison et al, 2004;Ruggiero and Procaccini, 2004;King and Roalson, 2008). In our study, the nucleotide sequences of ITS ranged from 720 bp in A. pskemense (W6 12754) to 748 bp in A. cernuum (W6 20304).…”
Section: Discussionmentioning
confidence: 99%
“…However, several species could not be discriminated from each other by either ITS or matK regions. On the other hand, the ITS region requires cloning before sequencing because of the allelic polymorphisms (presence of insertion/deletion, single nucleotide polymorphism), pseudogenes, and paralogous copies of the ITS region in a plant species (Buckler et al, 1997;Denduangboripant and Cronk, 2000;Kita and Ito, 2000;Bailey et al, 2003;Li et al, 2004;Razafimandimbison et al, 2004;Ruggiero and Procaccini, 2004;King and Roalson, 2008). In our study, the nucleotide sequences of ITS ranged from 720 bp in A. pskemense (W6 12754) to 748 bp in A. cernuum (W6 20304).…”
Section: Discussionmentioning
confidence: 99%
“…The region is bordered to the east by what is probably the most wellknown biogeographic boundary recognised today, Wallace's Line. A second but lesser-known biogeographic boundary occurs at the transition zone between the Sundaic and Indochinese biotas (sensu Woodruff, 2003) in the vicinity of the Isthmus of Kra (Figure 1), with distinct assemblages of amphibians (Inger, 1966; see review by Inger and Voris, 2001), reptiles (Inger and Voris, 2001), birds (Hughes et al, 2003), mammals (Corbett and Hill, 1992), insects (Corbet, 1941) and plants (Ridder-Numan, 1998;Denduangboripant and Cronk, 2000) limited to varying degrees either side of this barrier. Recently, it has been hypothesised that marine transgressions may have produced this pattern (Woodruff, 2003); specifically, that and Pliocene-era (5.5-4.5 Mya) high sea-level stands resulted in two seaways that dissected the Thai-Malay Peninsula (Figure 1; Woodruff, 2003), for durations in excess of 1 million years.…”
Section: Introductionmentioning
confidence: 99%
“…However, an increasing number of studies indicate that, in many taxa, the rate of homogenization may be too low to prevent significant levels of intraspecific rDNA polymorphisms. Often this variation is restricted to the noncoding rDNA regions such as the internal transcribed spacers (ITS1 and ITS2) or the intergenic spacer (IGS), which separates the transcribed units (Crease 1995;Copenhaver and Pikaard 1996;O'Donnell and Cigelnik 1997;Fenton et al 1998;Gernandt and Liston 1999;Hugall et al 1999;Denduangboripant and Cronk 2000;Harris and Crandall 2000;Whang et al 2002;Parkin and Butlin 2004). However, there are also numerous reports of rDNA pseudogenes where the coding regions are freed from functional constraints (Buckler et al 1997;Hartmann et al 2001;Mayol and Rossello 2001;Muir et al 2001;Márquez et al 2003;Razafimandimbison et al 2004;Ruggiero and Procaccini 2004).…”
mentioning
confidence: 99%