High‐level exogenous <i>trans</i>10, <i>cis</i>12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells

Wang, Hongfang; Liu, Hongyun; Liu, Jianxin; Zhao, Ke; Wang, Chong; Yang, Weiren

doi:10.1111/asj.12204

Cited by 11 publications

(14 citation statements)

References 24 publications

(58 reference statements)

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“…In the present study, 6 genes, including 4 DEG that regulate de novo fatty acid synthesis, triglyceride synthesis, and lipolysis (FASN, SCD1, ACSL4, and CPT1A), were verified using RT-qPCR. The findings of downregulation of FASN, ACACA, and SCD1 with an increasing concentration of t10c12-CLA were similar with those in bovine mammary epithelial cells (Kadegowda et al, 2009;Wang et al, 2014).…”

Section: Discussionsupporting

confidence: 77%

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trans-10,cis-12 conjugated linoleic acid alters lipid metabolism of goat mammary epithelial cells by regulation of de novo synthesis and the AMPK signaling pathway

Zhang

Huang

Tian

et al. 2018

Journal of Dairy Science

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The trans-10,cis-12 isomer of conjugated linoleic acid (t10c12-CLA) is a biohydrogenation intermediate in the rumen and has been shown to cause milk fat depression in dairy goats. However, few studies have focused on the in vitro molecular mechanisms involved in the response of the goat mammary gland to t10c12-CLA. In the present study, RNA sequencing technology was used to investigate the effects of t10c12-CLA on goat mammary epithelial cells. From the data, 25,153 annotated transcripts were obtained, and differentially expressed genes were selected based on a false discovery rate <0.05. Candidate genes and potent cellular signaling pathways were identified through Gene Ontology (GO) and pathway analysis. Next, real-time quantitative PCR and Western blot analyses were used to verify the results of the RNA sequencing data. The results indicated that t10c12-CLA inhibits fatty acid synthesis through downregulation of genes involved in de novo fatty acid synthesis, and this process is likely correlated with the activation of the AMP-activated protein kinase signaling pathways.

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Section: Discussionsupporting

confidence: 77%

“…A previous study reported that only a high dose of t10c12-CLA provides an anti-lipogenic effect (Wang et al, 2014). To investigate this further, we used GMEC as the in vitro model in this study, and tested 0, 10, and 200 µM t10c12-CLA to evaluate whether the effects was dose-dependent.…”

Section: Discussionmentioning

confidence: 98%

trans-10,cis-12 conjugated linoleic acid alters lipid metabolism of goat mammary epithelial cells by regulation of de novo synthesis and the AMPK signaling pathway

Zhang

Huang

Tian

et al. 2018

Journal of Dairy Science

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“…However, these results contrast research in primary bovine mammary epithelial cells in which t10c12‐CLA led to a decrease in TG content (Wang et al . ). In adipocytes, t10c12‐CLA inhibited differentiation (Kang et al .…”

Section: Discussionmentioning

confidence: 97%

“…In primary bovine mammary epithelial cells, a higher concentration of t10c12‐CLA (75–150 μmol/L) inhibited the messenger RNA (mRNA) expression of FASN , ACACA and SCD1 as well as reduced the triacylglycerol (TG) content (Wang et al . ).…”

Section: Introductionmentioning

confidence: 97%

Trans10, cis12 conjugated linoleic acid increases triacylglycerol accumulation in goat mammary epithelial cells in vitro

Zhang

Wang

et al. 2017

Animal Science Journal

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Trans10, cis12 conjugated linoleic acid (t10c12-CLA) is well-established in decreasing milk fat content and causing milk fat depression (MFD) in dairy cattle and goats. However, the detailed mechanisms of its effect are not completely understood. Therefore, we used goat mammary epithelial cells (GMECs) to further study the molecular mechanisms whereby t10c12-CLA regulates milk fat synthesis. The optimal concentration of t10c12-CLA (100 μmol/L) for cell culture was determined through a cell vitality and morphology assay, and evaluation of abundance of apoptosis-related proteins. Oil red O stain indicated that t10c12-CLA increased concentration of cytoplasmic lipid droplets. Furthermore, t10c12-CLA increased the intracellular triacylglycerol (TG) content (P < 0.05). Among 16 genes related to lipid metabolism that were measured by quantitative real-time PCR, t10c12-CLA down-regulated (P < 0.05) genes involved in de novo fatty acid synthesis including FASN, ACACA and SCD1, and also down-regulated the protein expression of FASN and SCD1 but up-regulated (P < 0.05) the expression of CD36 and ADRP. Overall, the data indicate that a side effect of de novo fatty acid synthesis inhibition by t10c12-CLA is the up-regulation of fatty acid uptake and accumulation of lipid droplets in GMECs. The biologic reason for such an effect merits further study.

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“…The CLA concentration (50 µM) was used because at 50 µM concentration, no differences were observed in cell viability between CLA treatments and control. Moreover, 3 previous studies have shown that higher concentrations of CLA induce apoptosis in the mammary cells (Keating et al, 2008;Wang et al, 2014). Finally, the physiological concentration of CLA in human sera is in the range of 10 to 70 µM (De la Torre et al, 2005).…”

Section: Experimental Designmentioning

confidence: 96%

Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1)

Basiricò

Morera

Dipasquale

et al. 2015

Journal of Dairy Science

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Some studies have shown the protective effects of conjugated linoleic acid (CLA) isomers against oxidative stress and lipid peroxidation in animal models, but no information is available about CLA and changes in oxidative status of the bovine mammary gland. The objectives of the study were to assess in vitro the effect of CLA on the cellular antioxidant response of bovine mammary cells, to examine whether CLA isomers could play a role in cell protection against the oxidative stress, and to study the molecular mechanism involved. For the study, BME-UV1 cells, a bovine mammary epithelial cell line, were used as the experimental model. The BME-UV1 cells were treated with complete medium containing 50 µM cis-9,trans-11 CLA (c9,t11 CLA), trans-10,cis-12 CLA (t10,c12 CLA), and CLA mixture (1:1, cis-9,trans-11: trans-10,cis-12 CLA). To monitor cellular uptake of CLA isomers, cells and culture medium were collected at 0, 3, and 48 h from CLA addition for lipid extraction and fatty acid analyses. To assess the cellular antioxidant response, glutathione (GSH/GSSH), NADPH, and γ-glutamyl-cysteine ligase activity was measured after 48 h from addition of CLA. Cytoplasmic superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and glutathione reductase activities and mRNA were also determined. Intracellular reactive oxygen species and thiobarbituric acid reactive substance production were assessed in cells supplemented with CLA isomers. Cell viability after 3h to H2O2 exposure was assessed to evaluate and to compare the potential protection of different CLA isomers against H2O2-induced oxidative stress. Mammary cells readily picked up all CLA isomers, their accumulation was time dependent, and main metabolites at 48 h are two 18:3 isomers. The CLA treatment induced an intracellular GSH increase, matched by high concentration of NADPH, and an increase of γ-glutamyl-cysteine ligase activity mainly in cells treated with the t10,c12 CLA isomer. The CLA isomer treatment of bovine mammary cells increased superoxide dismutase, glutathione peroxidase, and glutathione S-transferase activity and decreased glutathione reductase activity, but no changes in gene expression of these antioxidant enzymes were observed. Cells supplemented with CLA isomers showed a reduction in intracellular reactive oxygen species and thiobarbituric acid reactive substance levels. All CLA isomers were able to enhance cell resistance against H2O2-induced oxidative stress. These suggest an antioxidant role of CLA, in particular of t10,c12 CLA, by developing a significantly high redox status in cells.

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High‐level exogenous trans10, cis12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells

Cited by 11 publications

References 24 publications

trans-10,cis-12 conjugated linoleic acid alters lipid metabolism of goat mammary epithelial cells by regulation of de novo synthesis and the AMPK signaling pathway

trans-10,cis-12 conjugated linoleic acid alters lipid metabolism of goat mammary epithelial cells by regulation of de novo synthesis and the AMPK signaling pathway

Trans10, cis12 conjugated linoleic acid increases triacylglycerol accumulation in goat mammary epithelial cells in vitro

Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1)

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