High level expression of a recombinant xylanase by Pichia pastoris NC38 in a 5L fermenter and its efficiency in biobleaching of bagasse pulp

Birijlall, Natasha; Ayyachamy, Manimaran; Kumar, Kuttanpillai Santhosh; Permaul, Kugen; Singh, Surendra

doi:10.1016/j.biortech.2011.07.059

Cited by 31 publications

(16 citation statements)

References 31 publications

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“…The benefits obtained with xylanase are generally dependent on the chemical bleaching sequence used (Birijlall et al 2011;Viikari et al 1994 All experiments were performed at 10% pulp consistency and retention time of 60 min at 60…”

Section: Biobleaching Of Eucalyptus Kraft Pulp With Xylanase and Hydrmentioning

confidence: 99%

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Cheng¹,

Chen²,

Huang³

et al. 2013

BioResources

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In this work, a xylanase-producing strain, Streptomyces griseorubens LH-3, was cultured, and the crude xylanase was prepared. Analysis of its enzymatic properties revealed that the crude xylanase possessed good thermal stability at temperatures below 60 o C, exhibited a wide pH range from 4.0 to 9.0, and was cellulase-free. This crude enzyme was used to treat eucalyptus kraft pulp, and the release of chromophores was the highest at the dosage of 20 IU g −1 dry pulp. Compared with the untreated group, biobleaching of eucalyptus kraft pulp with this enzyme increased the brightness of the pulp by 12.9% and reduced the Kappa number by 27.4%. Biobleaching of eucalyptus kraft pulp with this enzyme obtained the same final pulp brightness compared with that of the control; however, hydrogen peroxide consumption was reduced by 17% and the yield and viscosity of the pulp was increased by 1.47% and 1.53%, respectively. This crude xylanase has promising potential for industrial applications.

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Section: Biobleaching Of Eucalyptus Kraft Pulp With Xylanase and Hydrmentioning

confidence: 99%

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Cheng¹,

Chen²,

Huang³

et al. 2013

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“…Xylanases have also been applied to biobleaching processes, as their removal of the xylan layer enhances the bleaching effect of chemical reagents (Birijlall et al 2011;Ko et al 2011;Valls and Roncero, 2009). Thus, numerous biobleaching studies using xylanases or laccases have been published (Chauhan et al 2006;Eugenio et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Improvement of TCF Bleaching of Olive Tree Pruning Residue Pulp by Addition of a Laccase and/or Xylanase Pre-Treatment

Martín‐Sampedro¹,

Rodríguez²,

Requejo³

et al. 2012

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aThis study aimed at assessing the biobleachability of soda pulps obtained from olive tree pruning residue. The enzymatic (LMS) pretreatment was applied prior to a simple totally chlorine free (TCF) bleaching sequence, consisting of an alkaline extraction and a hydrogen peroxide stage. Additionally, the effect of adding xylanase jointly with or prior to LMS was evaluated. All of these enzymatic pre-treatments were associated with an enhancement of the bleaching sequence. The best results were found when both enzymes were applied in the same stage: lowest hydrogen peroxide consumption (63 percent); kappa number, 11.6; brightness, 46 percent ISO. The mechanical properties observed were similar to those reported by other authors who have studied pulps from olive tree pruning residue. Finally, bleached pulps were subjected to accelerated ageing in order to assess the evolution of brightness and colorimetric properties. Although biobleached pulps showed lower stability upon ageing, the best optical properties, even after ageing, were observed in pulps treated with both xylanase and laccase.

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“…DEG75-AG can potentially be utilized in industrial processes that occur under alkaline conditions. For instance, DEG75-AG could be combined with alkaline-active endoxylanases in biobleaching of pulp to dramatically decrease the use of harsh chemicals [2,4,13].…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of a novel GH67 α-glucuronidase from a mixed culture

Lee

Kibblewhite

Wagschal

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Hemicelluloses represent a large reservoir of carbohydrates that can be utilized for renewable products. Hydrolysis of hemicellulose into simple sugars is inhibited by its various chemical substituents. The glucuronic acid substituent is removed by the enzyme α-glucuronidase. A gene (deg75-AG) encoding a putative α-glucuronidase enzyme was isolated from a culture of mixed compost microorganisms. The gene was subcloned into a prokaryotic vector, and the enzyme was overexpressed and biochemically characterized. The DEG75-AG enzyme had optimum activity at 45 °C. Unlike other α-glucuronidases, the DEG75-AG had a more basic pH optimum of 7-8. When birchwood xylan was used as substrate, the addition of DEG75-AG increased hydrolysis twofold relative to xylanase alone.

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High level expression of a recombinant xylanase by Pichia pastoris NC38 in a 5L fermenter and its efficiency in biobleaching of bagasse pulp

Cited by 31 publications

References 31 publications

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Improvement of TCF Bleaching of Olive Tree Pruning Residue Pulp by Addition of a Laccase and/or Xylanase Pre-Treatment

Isolation and characterization of a novel GH67 α-glucuronidase from a mixed culture

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