High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells

Ni, Hsiao Tzu; Hu, Shuxian; Sheng, Wen S.; Olson, Judy M.; Cheeran, Maxim C.-J.; Chan, Anissa; Lokensgard, James R.; Peterson, Phillip K.

doi:10.1016/j.devbrainres.2004.06.015

Cited by 73 publications

(84 citation statements)

References 58 publications

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“…CCR1 expression on U87MG cells was up-regulated by fourfold after treatment with IFN-␥, while GM-CSF had no effect on its expression. HFA were positive for CXCR4 (12%), which is consistent with previous reports (15,21), but its expression was not augmented by GM-CSF or IFN-␥ treatment (Fig. 4B).…”

supporting

confidence: 81%

Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes

Carroll-Anzinger

Al‐Harthi

2006

J Virol

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Considerable controversy exists over whether astrocytes can support human immunodeficiency virus (HIV) infection. We evaluated the impact of three cytokines critical to the development of HIV neuropathogenesis, gamma interferon (IFN-␥), granulocyte-macrophage colony-stimulating factor, and tumor necrosis factor alpha, on priming astrocytes for HIV infection. We demonstrate that IFN-␥ was the most potent in its ability to facilitate substantial productive HIV infection of an astroglioma cell line (U87MG) and human fetal astrocytes (HFA). The mechanism of IFN-␥-mediated priming of HIV in HFA is unlikely to be at the level of up-regulation of receptors and coreceptors relevant to HIV entry. These data demonstrate that cytokine priming can alter HIV replication in astrocytes.

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supporting

confidence: 81%

Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes

Carroll-Anzinger

Al‐Harthi

2006

J Virol

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“…Examination of the expression patterns of SDF-1 and CXCR4 during embryogenesis (McGrath et al, 1999;Stumm et al, 2002;Tissir et al, 2004) indicates that all of these developmental phenotypes can be explained on the basis of deficits in SDF-1 mediated attraction of embryonic neural stem/progenitor cells. In keeping with this possibility, examination of embryonic neural progenitor cells in culture has confirmed that these cells express CXCR4 receptors and that SDF-1 can act as a chemoattractant (Ji et al, 2004;Krathwohl and Kaiser, 2004;Ni et al, 2004;Peng et al, 2004;Tran et al, 2004). In addition to CXCR4, the CXCR2 chemokine receptor has been shown to be important for regulating the migration of oligodendrocyte progenitor cells in the developing spinal cord (Tsai et al, 2002).…”

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confidence: 99%

Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain

Tran

Banisadr

Ren

et al. 2006

J of Comparative Neurology

219

228

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We previously demonstrated that chemokine receptors are expressed by neural progenitors grown as cultured neurospheres. To examine the significance of these findings for neural progenitor function in vivo, we investigated whether chemokine receptors were expressed by cells having the characteristics of neural progenitors in neurogenic regions of the postnatal brain. Using in situ hybridization we demonstrated the expression of CCR1, CCR2, CCR5, CXCR3, and CXCR4 chemokine receptors by cells in the dentate gyrus (DG), subventricular zone of the lateral ventricle, and olfactory bulb. The pattern of expression for all of these receptors was similar, including regions where neural progenitors normally reside. In addition, we attempted to colocalize chemokine receptors with markers for neural progenitors. In order to do this we used nestin-EGFP and TLXLacZ transgenic mice, as well as labeling for Ki67, a marker for dividing cells. In all three areas of the brain we demonstrated colocalization of chemokine receptors with these three markers in populations of cells. Expression of chemokine receptors by neural progenitors was further confirmed using CXCR4-EGFP BAC transgenic mice. Expression of CXCR4 in the DG included cells that expressed nestin and GFAP as well as cells that appeared to be immature granule neurons expressing PSA-NCAM, calretinin, and Prox-1. CXCR4-expressing cells in the DG were found in close proximity to immature granule neurons that expressed the chemokine SDF-1/CXCL12. Cells expressing CXCR4 frequently coexpressed CCR2 receptors. These data support the hypothesis that chemokine receptors are important in regulating the migration of progenitor cells in postnatal brain. Indexing terms stem cells; development; chemotaxis; brain repairDuring embryogenesis, neural stem/progenitor cells must migrate long distances from the germinal epithelia where they are born to their final destinations (Hatten, 1999;Alvarez-Buylla et al., 2001). During migration, stem cells may continue to divide and also become restricted in terms of their ultimate phenotypes. Thus, the timing of neuro-and gliogenesis in the developing embryo is precisely controlled (Rallu et al., 2002). The factors that determine the migration, proliferation, and differentiation of embryonic stem cells have been widely investigated and numerous factors have been shown to influence these processes (Lindvall et al., 2004). One group of molecules that has recently been shown to control progenitor cell migration in the nervous system are the chemokines (CHEMO-tactic cytoKINES) (Tran and © NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript Miller, 2003). The chemokines are a family of small secreted proteins that are known to be important regulators of leukocyte trafficking under both normal conditions and during inflammatory responses (Moser et al., 2004). Furthermore, it is now known that chemokine signaling has an important role to play in the developing embryo. Deletion of the genes for the CXCR4 chemokine receptor or f...

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“…Therefore, we asked all authors to revisit the table and upgrade their part either by own data or based on additional literature, as necessary. This resulted in the upgraded Table 1 showing phenotypes of very small embryonic-like stem cells (vSELs) (5,6), neural stem cells (NSCs) (7)(8)(9)(10)(11)(12)(13)(14)(15)(16), hematopoietic stem cells (HSCs) from two organs (4,17), MSCs (4,18), EpSC (4), limbal epithelial stem cells (LSCs) (19), endothelial progenitor cells (EPCs) from different organs (3,4,(20)(21)(22)(23)(24)(25), supra-adventitial adipose stromal cells (SA-ASCs) (3,(22)(23)(24)(25), adipose pericytes (pericyte) (3,(22)(23)(24)(25), and finally cancer stem cells (CSCs) (26).…”

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confidence: 99%

Phenotypes of stem cells from diverse origin

2009

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High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells

Cited by 73 publications

References 58 publications

Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes

Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes

Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain

Phenotypes of stem cells from diverse origin

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