High-level mRNA quantification of proliferation marker pKi-67 is correlated with favorable prognosis in colorectal carcinoma

Ihmann, Thomas; Liu, Jian; Schwabe, Wolfgang; Häusler, Peter; Behnke, Detlev; Bruch, Hans–Peter; Broll, R.; Windhövel, Ute; Duchrow, M.

doi:10.1007/s00432-004-0612-5

Cited by 16 publications

(13 citation statements)

References 32 publications

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“…Two genes, BUB1B and CDC20, outperformed Ki-67 RNA. This is consistent with past reports for colorectal carcimona in whichMIB-1 Li had no significant correlation with Ki-67 mRNA expression [17], [53] …”

Section: Discussionsupporting

confidence: 93%

“…We also measuredKi-67 RNA levels because the corresponding protein is currently used to determine a labeling index for glioma [6], [7], [8], [9], [10], [11], [12], [13], [14], [15], [16] and previous work indicated the RNA might be a better prognosticator [17]. Interestingly, Ki-67 RNA level was outperformed by BUB1B and CDC20, overall, and by BUB1 and TTK when applied to grade II gliomas ( Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, one report indicated that RNA expression of Ki-67 correlated with survival time better than the antibody test, MIB Li, in colorectal carcinoma [17].…”

Section: Introductionmentioning

confidence: 99%

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The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression

et al. 2011

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Identification of gene expression changes that improve prediction of survival time across all glioma grades would be clinically useful. Four Affymetrix GeneChip datasets from the literature, containing data from 771 glioma samples representing all WHO grades and eight normal brain samples, were used in an ANOVA model to screen for transcript changes that correlated with grade. Observations were confirmed and extended using qPCR assays on RNA derived from 38 additional glioma samples and eight normal samples for which survival data were available. RNA levels of eight major mitotic spindle assembly checkpoint (SAC) genes (BUB1, BUB1B, BUB3, CENPE, MAD1L1, MAD2L1, CDC20, TTK) significantly correlated with glioma grade and six also significantly correlated with survival time. In particular, the level of BUB1B expression was highly correlated with survival time (p<0.0001), and significantly outperformed all other measured parameters, including two standards; WHO grade and MIB-1 (Ki-67) labeling index. Measurement of the expression levels of a small set of SAC genes may complement histological grade and other clinical parameters for predicting survival time.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression

et al. 2011

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“…It has a role in cell division by regulating cell cycle (30). In this investigation, there was no significant difference in Ki-67 expression between treated KB cells with different concentrations of Frankincense extract and untreated cells.…”

Section: Discussionmentioning

confidence: 48%

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Jaafari‐Ashkavandi¹,

Hamedi²,

Assar³

et al. 2017

Int J Cancer Manag

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Background: Cytotoxic effects of Frankincense resin have been shown on some cancer cell lines. Due to its low side effects, this study was designed to evaluate the anticancer properties of water soluble elements of Frankincense oleo-gum-resin on human oral squamous cell carcinoma cell line. Methods:Oleo-gum-resin was macerated in ethanol. After filtration, the water soluble fraction of dried residue was extracted. KB cells were treated with 0, 62.5, 125, 250 and 500 µg/mL concentrations of obtained Frankincense aqueous fractions and with Doxorubicin as positive control. Frankincense induced cell cytotoxicity; apoptosis and proliferation were investigated using WST assay, Annexin V-FITC/PI, and Ki-67 staining, respectively. Data were analyzed using Kruskal-Wallis test by SPSS 17 software.Results: IC50 of 137.21 µg/mL was obtained from Frankincense aqueous fraction after 48 hours. The percentage of apoptotic cells was elevated in a time-and dose-dependent manner. There was no statistical difference in the Ki-67 expression of KB cells, using different concentration of Frankincense aqueous fraction after 24 and 48 hours (P = 0.083). Doxorubicin inhibited cells growth essentially through apoptosis. Conclusions:Frankincense aqueous fractions seem to suppress KB cell growth through the induction of apoptosis and necrosis rather than the inhibition of proliferation and hence might be a potential anticancer agent. Structural analysis and purification of potent components are suggested for determining more definitive results.

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“…PCNA is increased in late G 1 and S phases of the cell cycle, and is correlated with the rate of DNA synthesis and cellular proliferation (28). In addition, Ki-67 is associated with cell proliferation and is detected throughout the cell cycle (29,30). The differences observed between the extent of increase of PCNA and Ki-67 levels suggest that these antigens may be differentially affected by NY-SAR-35 expression, which may be due to the fact that they are expressed differently in different cell types (31).…”

Section: Discussionmentioning

confidence: 99%

Effect of cancer/testis antigen NY-SAR-35 on the proliferation, migration and invasion of cancer cells

et al. 2016

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NY-SAR-35 is a cancer/testis (CT) antigen that was identified by serological analysis of recombinant complementary DNA expression libraries. The gene encoding NY-SAR-35 is located on the × chromosome and is aberrantly expressed in a number of cancer types and germ cells, such as those in the testes, but not in normal tissue. It has been reported that treatment with a demethylating agent induced the expression of NY-SAR-35 in several types of cancer cells. However, the function of NY-SAR-35 in cancer remains undetermined. In present study, the role of NY-SAR-35 in human lung adenocarcinoma (SK-LC-14) and hepatocellular carcinoma (SNU-449) cells was investigated following stable transfection of the NY-SAR-35 gene. NY-SAR-35 was observed to be expressed in the cytoplasm of the cells. In addition, the bromodeoxyuridine incorporation assay and immunofluorescence staining for proliferating cell nuclear antigen and Ki-67 demonstrated that proliferation was increased in cells transfected with NY-SAR-35. In addition, the trypan blue exclusion assay indicated that NY-SAR-35 increased cancer cell viability. Furthermore, NY-SAR-35 increased the migration and invasion of the cells. These results indicate that NY-SAR-35 increases cancer cell viability, proliferation, migration and invasion.

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High-level mRNA quantification of proliferation marker pKi-67 is correlated with favorable prognosis in colorectal carcinoma

Abstract: Quantitatively determined pKi-67 mRNA can be a good and new prognostic indicator for primary resected colorectal carcinoma.

Cited by 16 publications

References 32 publications

The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression

The Accuracy of Survival Time Prediction for Patients with Glioma Is Improved by Measuring Mitotic Spindle Checkpoint Gene Expression

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Effect of cancer/testis antigen NY-SAR-35 on the proliferation, migration and invasion of cancer cells

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