High level of metaflumizone resistance and multiple insecticide resistance in field populations of Spodoptera exigua (Lepidoptera: Noctuidae) in Guangdong Province, China

Su, Jianya; Sun, Xing-Xing

doi:10.1016/j.cropro.2014.03.013

Cited by 60 publications

(45 citation statements)

References 25 publications

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“…However, the expression and regulation of detoxification genes are less well reported in Spodoptera exigua (Hübner), which is a worldwide pest that damages numerous crops, including vegetables and ornamentals. Abamectin, lambda‐cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb have been extensively applied to control S. exigua in China, and very high resistance has been reported in field populations from Guangdong province in South China (Su & Sun, ; Tian et al ., ). The enhanced breakdown of insecticides in resistance populations partially mediated these resistances, and increased P450 activity was the primary cause for metabolic resistance in this pest (Shimada et al ., ; Tian et al ., ; Wang et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

et al. 2017

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Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can increase the expression of detoxification enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells; eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression; more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In conclusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification.

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Section: Introductionmentioning

confidence: 99%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

et al. 2017

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“…The HZ12‐meta strain had enhanced FMO (1.5‐fold), esterase (3.4‐fold) and P450 (1.4‐fold) enzymatic activities compared to the susceptible strain, and the enzyme inhibitors significantly synergized the toxicity of metaflumizone on the HZ12‐meta strain: the synergism ratios were 1.9, 3.4 and 1.7 for FMO, esterase and P450, respectively. Therefore, the increased detoxification may play some role in this resistance; even the target mutation for this resistance remains undefined at present (Su & Sun, ; Tian et al ., ). According to the increased enzymatic activities and the inhibitors' synergistic potentials in the HZ12‐meta strain, metabolic resistance may be conferred mainly by esterases and to a lesser extent by FMOs and P450s in this strain.…”

Section: Discussionmentioning

confidence: 97%

“…Insecticide resistance is generally conferred by an increase in detoxification metabolism and a decrease in target binding affinity in the resistant population (Li et al ., ; Dong et al ., ; Tian et al ., ). Our previous work showed that S. exigua populations collected from Huizhou in 2011 and 2012 had developed resistance (over 60‐fold) to a novel insecticide, metaflumizone (Su & Sun, ), which is a blocker of voltage‐gated sodium channels (Silver et al ., ). Studies on biochemical mechanism revealed that the S. exigua population resistant to metaflumizone had higher cytochrome P450 (P450; 1.4‐fold) and esterase (3.4‐fold) activities than the susceptible strain (Lab‐sus), and the inhibitors of esterase and P450 synergize the toxicity of metaflumizone against the resistant strain (HZ12‐meta).…”

Section: Introductionmentioning

confidence: 97%

“…It has developed high levels of resistance to multiple insecticides owing to sustained repetitive insecticide application, and control failure has been reported for many insecticides in south China, such as metaflumozine, lambda‐cyhalothrin, chlorpyrifos, abamectin, chlorantraniliprole, etc. (Su & Sun, ). Cases of field resistance to many conventional chemicals (including organophosphates, carbamates and pyrethroids) and novel chemicals (including spinosad, abamectin, indoxacarb and tebufenozide) in other areas of the world have also been reported (Moulton et al ., ; Osorio et al ., ; Lai et al ., ; Ishtiaq et al ., ; Che et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization, expression pattern and metabolic activity of flavin‐dependent monooxygenases inSpodoptera exigua

Tian

Zhao²,

Guo

et al. 2018

Insect Molecular Biology

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Enhanced detoxification is one of the important mechanisms for insecticide resistance. Most research in this field to date has focused on the role of cytochrome P450s. Our previous work revealed that flavin-dependent monooxygenases (FMOs) were involved in metabolic resistance of Spodoptera exigua. In the present study we investigated the molecular characteristics, expression patterns and oxidative activities of SeFMO on insecticides. Three FMO genes, which encode proteins with the typical FMO motifs, were cloned from S. exigua. The oxidative activities of eukaryotically expressed SeFMO enzymes were verified with the model substrate of FMO. Importantly, the SeFMOs had significantly higher oxidative activities on metaflumizone and lambda-cyhalothrin than on model substrates and other insecticides tested. The three SeFMOs were mainly expressed in the midgut, fat body and Malpighian tubules. The tissues responsible for xenobiotic metabolism and their expression characteristics were similar to those of P450s acting as detoxification genes. The study also revealed that the expression of SeFMOs could be induced by insecticide exposure, and that SeFMOs were over-expressed in a metaflumizone-resistant strain of S. exigua. These results suggest that SeFMOs are important insecticide detoxifying enzymes, and that over-expression of FMO genes may be one of the mechanisms for metabolic resistance in S. exigua.

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“…The most common adaptive mechanism may be straightforward insecticide resistance. The beet armyworm, for example, has evolved over 3,000-fold resistance to the pyrethroid insecticide lambda cyhalothrin9. In extreme cases, insecticide resistance can lead to local disasters.…”

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confidence: 99%

Driving Pest Insect Populations: Agricultural Chemicals Lead to an Adaptive Syndrome in Nilaparvata Lugens Stål (Hemiptera: Delphacidae)

You

et al. 2016

Sci Rep

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The brown planthopper (BPH) is a devastating pest of rice throughout Asia. In this paper we document the BPH biogeographic range expansion in China over the 20-year period, 1992 to 2012. We posed the hypothesis that the range expansion is due to a syndrome of adaptations to the continuous presence of agricultural chemicals (insecticides and a fungicide) over the last 40 years. With respect to biogeography, BPH ranges have expanded by 13% from 1992 to 1997 and by another 3% from 1997 to 2012. In our view, such expansions may follow primarily from the enhancing effects of JGM, among other agricultural chemicals, and from global warming. JGM treatments led to increased thermotolerance, recorded as decreased mortality under heat stress at 40 ± 1 °C (down from 80% to 55%) and increased fecundity (by 49%) at 34 °C. At the molecular level, JGM treatments led to increased abundances of mRNA encoding Acetyl Co-A carboxylase (Acc) (up 25%) and Hsp70 (up 32%) in experimental BPH. RNAi silencing of Hsp70 and Acc eliminated the JGM effects on fecundity and silencing Hsp70 reduced JGM-induced thermotolerance. Integrated with global climate change scenarios, such syndromes in pest insect species have potential for regional- and global-scale agricultural disasters.

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High level of metaflumizone resistance and multiple insecticide resistance in field populations of Spodoptera exigua (Lepidoptera: Noctuidae) in Guangdong Province, China

Cited by 60 publications

References 25 publications

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Molecular characterization, expression pattern and metabolic activity of flavin‐dependent monooxygenases inSpodoptera exigua

Driving Pest Insect Populations: Agricultural Chemicals Lead to an Adaptive Syndrome in Nilaparvata Lugens Stål (Hemiptera: Delphacidae)

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