High-level secretion of the extracellular domain of the human growth hormone receptor using a baculovirus system

An extracellular portion of granulocyte colony-stimulating factor (G-CSF) receptor, which contains an immunoglobulin-like (Ig) domain and cytokine receptor homologous (CRH) region, was secreted into the medium using Trichoplwia ni-Autographa californica nuclear polyhedrosis virus system. The gene product was purified to homogeneity mainly as a dimer (85 kDa) using G-CSF affinity column chromatography and gel filtration HPLC, although the product existed as a monomer (45 kDa) in the medium. Scatchard analyses suggested that only the dimer had high aBinity ligand binding (& = about 100 PM), which is comparable with the Kd value of the cell surface. receptor. The binding of G-CSF to Ig-CRH induced its tetramerization (200-250 kDa). The molecular composition of the tetrameric complex showed a stoichiometry of four ligands bound to four Ig-CRH. These results suggested that the oligomeric mechanism of the G-CSF receptor differs from that reported for growth hormone (GH) receptor, although CD spectrum spectroscopy suggested that the Ig-CRH has a GH receptor-like structure.

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The enhancement of the extracellular carboxyl-terminal domain of human growth hormone receptor on growth hormone dependent responses of 3T3-F442A cells

Akira¹,

Kikuchi²,

Uchida³

et al. 1994

Biomedicine & Pharmacotherapy

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High-level secretion of the extracellular domain of the human growth hormone receptor using a baculovirus system

Cited by 9 publications

References 12 publications

Expression of the extracellular domain of the human growth hormone receptor gene by an insect-baculovirus system

Expression of the extracellular domain of the human growth hormone receptor gene by an insect-baculovirus system

Evidence for the ligand‐induced conversion from a dimer to a tetramer of the granulocyte colony‐stimulating factor receptor

The enhancement of the extracellular carboxyl-terminal domain of human growth hormone receptor on growth hormone dependent responses of 3T3-F442A cells

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