High levels of corticosterone, and gene expression of star, cyp17a2, hsd3b, cyp21, hsd11b2 during acute stress in common carp with interrenal hyperplasia

Nematollahi, Mohammad Ali; Pelt-Heerschap, H.M.L. van; Atsma, W.; Komen, J.

doi:10.1016/j.ygcen.2012.01.023

Cited by 14 publications

(8 citation statements)

References 44 publications

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“…Because the 11-beta hydroxysteroid dehydrogenase inactivates cortisol, it would be expected that higher levels of hsd11b2 could mitigate glucocorticoid signaling in the stressed group. However, this lower level of expression was consistent with decreased hsd11b2 that was found 22–24 hours after acute stress studies in adult zebrafish brain and in the kidney of a common carp strain 12,40 . Alderman and Vijayan 12 suggested that turnover to protein could decrease hsd11b2 mRNA levels.…”

Section: Discussionsupporting

confidence: 85%

Telencephalon transcriptome analysis of chronically stressed adult zebrafish

Huang

Butler

Lubin

2019

Sci Rep

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Chronic stress leads to disruptions in learning and memory processes. The effects of chronic stress experience on the adult zebrafish brain, particularly the memory associated telencephalon brain region, is unclear. The goal of this study was to identify gene expression changes in the adult zebrafish brain triggered by chronic unpredictable stress. Transcriptome analysis of the telencephalon revealed 155 differentially expressed genes. Of these genes, some are critical genes involved in learning and memory, such as cdk5 and chrna7, indicating effects of chronic unpredictable stress on zebrafish memory. Interestingly, several genes were annotated in the Orange domain, which is an amino acid sequence present in eukaryotic DNA-binding transcription repressors. Furthermore, we identified hsd11b2, a cortisol inactivating gene, as chronic stress-responsive in the whole zebrafish brain. Collectively, these findings suggest that memory associated gene expression changes in adult zebrafish telencephalon are affected by chronic stress experience.

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Section: Discussionsupporting

confidence: 85%

Telencephalon transcriptome analysis of chronically stressed adult zebrafish

Huang

Butler

Lubin

2019

Sci Rep

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“…Hsd11b2 plays a role in the conversion of the physiologically active cortisol to inactive cortisone [51]. It has been suggested that 11ß-hydroxysteroid dehydrogenases protect the teleost gonads from the inhibitory effects of cortisol, such as the inhibition of testicular androgen production [52,53].…”

Section: Resultsmentioning

confidence: 99%

Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer)

Ravi

Jiang

Liew

et al. 2014

Reprod Biol Endocrinol

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BackgroundThe Asian seabass (Lates calcarifer) is a protandrous hermaphrodite that typically matures as a male at approximately 2–4 years of age and then changes sex in subsequent years. Although several sexual maturation stages have been described histologically for both testis and ovary, the underlying gene expression profiles remain lacking. The development of a gene expression platform is therefore necessary to improve our understanding of the gonad development of this cultured teleost species.MethodsThirty Asian seabass gonads were collected from farms in Singapore, examined histologically and staged according to their sex and gonadal maturation status. Partial coding sequences of 24 sex-related genes were cloned using degenerate primers and were sequenced. Additional 13 cDNA sequences were obtained through next-generation sequencing. A real-time qPCR was then performed using the microfluidic-based Fluidigm 48.48 Dynamic arrays.ResultsWe obtained 17 ovaries and 13 testes at various stages of sexual maturation. Of the 37 genes that were tested, 32 (86%) showed sexually dimorphic expression. These genes included sex-related genes, sox9, wt1, amh, nr5a2, dmrt1 and nr0b1, which showed testis-enhanced expression similar to other vertebrate species. Known male- and female-enhanced germ cells markers, which were established from studies in other species, similarly showed testis- and ovary-enhanced expression, respectively, in the Asian seabass. Three pro-Wnt signaling genes were also upregulated in the ovary, consistent with existing studies that suggested the role of Wnt signaling in ovarian differentiation in teleosts and mammals. The expression patterns of genes involved in steroidogenesis, retinoic acid metabolism, apoptosis and NF-κB signaling were also described. We were able to classify gonads according to sex and gonadal maturation stages, based on their small-scale transcriptomic profiles, and to uncover a wide variation in expression profiles among individuals of the same sex.ConclusionsThe analysis of a selected set of genes related to reproduction and in sufficient number of individuals using a qPCR array can elucidate new insights into the molecular mechanisms involved in Asian seabass gonad development. Given the conservation of gene expression patterns found in this study, these insights may also help us draw parallels with other teleosts.

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“…Alternatively, based on a time-delayed correlation between cortisol release and 11b-hydroxysteroid dehydrogenase 2 (hsd11b2) transcript increases, several studies have suggested that hsd11b2 could be responsible for mediating the ultra-short loop negative feedback mentioned earlier. Hsd11b2, an enzyme that oxidizes cortisol to the inactive cortisone, is postulated to be involved in significantly reducing cortisol concentrations in the interrenal cells, thus reducing the amount of cortisol secreted [12,13,30,31]. Further investigation is needed to confirm these theories for teleosts and whether or not these mechanisms are GR-mediated.…”

Section: Discussionmentioning

confidence: 99%