High Linoleic Acid, Low Vegetable, and High Oleic Acid, High Vegetable Diets Affect Platelet Activation Similarly in Healthy Women and Men

Abstract

Upregulation of protein kinase C (PKC), an important enzyme in platelet activation, could be one step toward platelet hyperactivity. PKC activation can be modulated by dietary components in vitro, but few data are available concerning the in vivo effects. In this strictly controlled human dietary intervention, the influence of dietary unsaturated fatty acids and vegetable compounds on platelet activation was investigated. A high linoleic acid diet (10% of energy) with small amounts of vegetables (no berries or apples) was consumed by 9 women and 4 men (24.1 +/- 3.9 y), and was compared with a high oleic acid diet (12% of energy) with considerable amounts of vegetables, berries and apples consumed by 8 women and 4 men (24.2 +/- 5.5 y). Subjects were healthy Finnish volunteers. Compliance with the experimental protocol was good, as indicated by changes in plasma fatty acids and concentrations of vitamin C, beta-carotene and alpha-tocopherol. No differences between groups were seen in indices of platelet activation, including platelet aggregation, total PKC activity and distribution of PKC isoenzymes alpha, beta(II) and delta. The results indicate that in apparently healthy and fairly young subjects with adequate vitamin intakes, diets differing markedly in their amounts of linoleic and oleic acids, and vegetables, berries and apples do not differ in platelet activation.

Extra virgin olive oil: More than a healthy fat

“…TXA2 has an important role in the progression of cardiovascular disease due to its acute and chronic effect in promoting platelet aggregation, vasoconstriction and cellular proliferation. However, differential effects of MUFA-rich diets with those rich in PUFA were not clear, suggesting controversial benefit effects on platelet activation comparing these type of oils [71].…”

Olive Oil‐Based Lipid Emulsions Do Not Influence Platelet Receptor Expression in Comparison to Medium and Long Chain Triglycerides In vitro

“…for intensive care patients. Several studies examined the influence of lipid emulsions at homeostasis [8,9,11,15] but, in this study, the influence of different lipid emulsions on platelet receptor expression was examined for the first time.…”

“…The influence of lipid emulsions on platelet function and a modulation of coagulation is a controversial issue: some studies describe an impediment of the coagulation and platelet aggregation after consuming high-linoleic acid diets [7] or after an intravenous infusion of triglycerides [8]. Other studies had not found an influence of lipid emulsions containing n-3 fatty acid on coagulation and platelet function [9,10] or after a diet with high linoleic acid vs high oleic acid [11]. The structure of cell membranes seems to be modified by parenteral nutrition with lipid emulsions.…”

Olive oil and the haemostatic system

“…Misikangas et al found no differences in platelet aggregation or in protein kinase C (an important platelet regulator) induced by a diet high in linoleic acid but low in vegetables, as against one that was high in oleic acid and also vegetable-rich, although there is a certain complicating factor in interpreting this study due to the mixing of the potential effects of the fatty acids with those of the vegetables. Furthermore, the authors did not identify the origin of the oleic acid they employed [20].…”