High magnesium inhibits human osteoblast differentiation in vitro

Leidi, Marzia; Dellera, F.; Mariotti, Massimo; Maier, Jeanette A.M.

doi:10.1684/mrh.2011.0271

Cited by 122 publications

(91 citation statements)

References 19 publications

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“…We found that HMEC organize in tubes to the same extent and following the same kinetics in 0.1 and 1.0 mM Mg, thus indicating that Mg levels do not influence endothelial differentiation. Analogously, in pre-osteoblasts induced to differentiate we found no modulation of the differentiative process in 0.1 and 1.0 mM Mg [36]. We also show that TRPM7 is not involved in tridimensional organization of HMEC.…”

Section: Discussionsupporting

confidence: 53%

Silencing TRPM7 mimics the effects of magnesium deficiency in human microvascular endothelial cells

Baldoli¹,

Maier²

2011

Angiogenesis

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Evidence has accumulated to suggest that magnesium might play a role in controlling angiogenesis. Since microvascular endothelial cells are protagonists in this process, we investigated the behavior of these cells cultured in low extracellular magnesium or silenced for its transporter Transient Receptor Potential Melastatin (TRPM)7, essential for cellular magnesium homeostasis. In particular, we focused on some crucial steps of the angiogenic process, i.e. proliferation, migration, protease production and organization in tridimensional structures. Silencing TRPM7 mimics the effects of low extracellular magnesium on human microvascular endothelial cells (HMEC). Indeed, while no effects were observed on the production of metalloproteases and on tridimensional organization on matrigel, both magnesium deficiency and silencing of TRPM7 impair cell migration and inhibit growth by arresting the cells in the G0/G1 and G2/M phases of the cell cycle. Since low extracellular magnesium markedly decreases TRPM7 in HMEC, we suggest that TRPM7 downregulation might mediate low magnesium-induced inhibition of cell growth and migration. Human endothelial cells from the umbilical vein are growth inhibited by low magnesium and growth stimulated after TRPM7 silencing. An impairment of ERK phosphorylation in HMEC silencing TRPM7 is responsible, in part, for the different proliferative behavior of these two cell types. We broadened our studies also to endothelial colony-forming cells and found that they are sensitive to fluctuations of the concentrations of extracellular magnesium, while their proliferation rate is not modulated by TRPM7 silencing. Our results point to magnesium and TRPM7 as a modulators of the angiogenic phenotype of microvascular endothelial cells.

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Section: Discussionsupporting

confidence: 53%

Silencing TRPM7 mimics the effects of magnesium deficiency in human microvascular endothelial cells

Baldoli¹,

Maier²

2011

Angiogenesis

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“…Doping Mg in HA (2 mol%) had been shown to significantly enhance osteoblast adhesion as compared to undoped one [45] while increasing its concentration (20 wt%) was found to reduce the osteoconductivity of osteoblast-like cells [46]. Toxic effect of high Mg concentration reported in the literature [39,40] might, therefore, be one of the possible reasons for low cell proliferation above 10 at%. Besides this, increasing the Mg amount in the (Ti,Mg)N thin film coatings above 10 at% was shown to lead corrosion [47].…”

Section: Ha-deposited Tin and (Timg)n Thin Film Coatingsmentioning

confidence: 97%

“…While low amount of Mg make an insulin-like effect on cell proliferation, its high amount has shown to have negative impact on cell growth [39,40]. This necessitates the tuning of Mg in the coatings and for this, the coatings were deposited on titanium substrates by cathodic arc physical vapor deposition technique and HA formation was achieved in simulated body fluids (1Â SBF, 5Â SBF) at 378C.…”

Section: Introductionmentioning

confidence: 99%

Behavior of mammalian cells on magnesium substituted bare and hydroxyapatite deposited (Ti,Mg)N coatings

et al. 2015

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“…Normal human osteoblasts (NHOst) were maintained in osteoblast growth media (OGM) as indicated by the manufacturer (Lonza, Basel, Switzerland) at 37°C in a humidified atmosphere containing 5% CO 2 [18]. Saos-2 cells (American Type Culture Collection) were cultured in DMEM containing 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

“…Osteoblast activity was evaluated quantifying alkaline phosphatase (ALP) enzymatic activity in the medium by a colorimetric assay based on the hydrolysis of P-nitrophenyl phosphate. The absorbance was measured at 405 nm [18]. To analyze calcium deposition, the cells were rinsed with PBS, fixed (70% ethanol, 1 h), and stained for 10 min with 2% Alizarin Red S (pH 4.2).…”

Section: Methodsmentioning

confidence: 99%

Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity

Cazzaniga

Castiglioni

Maier

2014

BioMed Research International

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Background and Aims. Gravity contributes to the maintenance of bone integrity. Accordingly, weightlessness conditions during space flight accelerate bone loss and experimental models in real and simulated microgravity show decreased osteoblastic and increased osteoclastic activities. It is well known that the endothelium and bone cells cross-talk and this intercellular communication is vital to regulate bone homeostasis. Because microgravity promotes microvascular endothelial dysfunction, we anticipated that the molecular cross-talk between endothelial cells exposed to simulated microgravity and osteoblasts might be altered. Results. We cultured human microvascular endothelial cells in simulated microgravity using the rotating wall vessel device developed by NASA. Endothelial cells in microgravity show growth inhibition and release higher amounts of matrix metalloproteases type 2 and interleukin-6 than controls. Conditioned media collected from microvascular endothelial cells in simulated microgravity were used to culture human osteoblasts and were shown to retard osteoblast proliferation and inhibit their activity. Discussion. Microvascular endothelial cells in microgravity are growth retarded and release high amounts of matrix metalloproteases type 2 and interleukin-6, which might play a role in retarding the growth of osteoblasts and impairing their osteogenic activity. Conclusions. We demonstrate that since simulated microgravity modulates microvascular endothelial cell function, it indirectly impairs osteoblastic function.

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High magnesium inhibits human osteoblast differentiation in vitro

Cited by 122 publications

References 19 publications

Silencing TRPM7 mimics the effects of magnesium deficiency in human microvascular endothelial cells

Silencing TRPM7 mimics the effects of magnesium deficiency in human microvascular endothelial cells

Behavior of mammalian cells on magnesium substituted bare and hydroxyapatite deposited (Ti,Mg)N coatings

Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity

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