High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

Qian, Airong; Yang, Pengfei; Hu, Lifang; Zhang, Wen; Di, Shengmeng; Wang, Z.; Han, Jian; Gao, Xiang; Shang, Peng

doi:10.1016/j.asr.2010.04.012

Cited by 27 publications

(20 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies and those of other researchers have shown that cellular cytoskeleton of bone cells can rearrange to adapt to mechanical environments (Malone et al, 2007;McGarry et al, 2005;Ponik et al, 2007;Qian et al, 2010;Qian et al, 2012). Here, we demonstrated that fl uid fl ow stress causes a remarkable increase in β-actin and α-tubulin expression at mRNA level, and promotion in stress fi ber formation in MLO-Y4 cells, not in MC3T3-E1 cells.…”

Section: Integrin-associated Molecules Expression At Mrna Levelsupporting

confidence: 51%

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Zhang

et al. 2012

Biol. Res.

View full text Add to dashboard Cite

Interstitial fl uid fl ow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fl uid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fl uid fl ow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a signifi cant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin αvβ3. However, there was no or limited increase observed in MC3T3-E1 osteoblast-like cells. Cell area and fi ber stress formation were also markedly promoted by fl uid fl ow only in MLO-Y4 cells. But the numbers of processes per cell remain unaff ected in both cell lines.

show abstract

Section: Integrin-associated Molecules Expression At Mrna Levelsupporting

confidence: 51%

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Zhang

et al. 2012

Biol. Res.

View full text Add to dashboard Cite

show abstract

“…Cellular morphology and membrane roughness of bone-inducing cells were shown to be associated with the presence of mechanical loads, as the removal of gravitational loads with high magnitude of environmental gradient (0 g using magnetic levitation) reduced the height and the roughness of these cells (Qian et al, 2010). Our results suggest that daily addition of oscillatory loads increases the roughness of the cellular membrane for marrow stem cells that commit to osteogenesis.…”

Section: Discussionmentioning

confidence: 58%

“…We were not able to achieve the scanning of live cells in "fluid cell apparatus", which is a physiologically more relevant test for cellular membranes (Pesen and Hoh, 2005). Therefore, our results regarding cell morphology and roughness should be treated as relative rather than absolute (Qian et al, 2010). Mechanical vibrations were shown to be anabolic for osteoprogenitor cell pools in vitro, whether the application of stimulus was vertical (Kim et al, 2012) or horizontal (Uzer et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Bone marrow stem cells adapt to low-magnitude vibrations by altering theircytoskeleton during quiescence and osteogenesis

Demiray¹,

Özçivici

2015

Turk J Biol

View full text Add to dashboard Cite

Application of mechanical vibrations is anabolic to bone tissue, not only by guiding mature bone cells to increased formation, but also by increasing the osteogenic commitment of progenitor cells. However, the sensitivity and adaptive response of bone marrow stem cells to this loading regimen has not yet been identified. In this study, we subjected mouse bone marrow stem cell line D1-ORL-UVA to daily mechanical vibrations (0.15 g, 90 Hz, 15 min/day) for 7 days, both during quiescence and osteogenic commitment, to identify corresponding ultrastructural adaptations on cellular and molecular levels. During quiescence, mechanical vibrations significantly increased total actin content and actin fiber thickness, as measured by phalloidin staining and fluorescent microscopy. Cellular height also increased, as measured by atomic force microscopy, along with the expression of focal adhesion kinase (PTK2) mRNA levels. During osteogenesis, mechanical vibrations increased the total actin content, actin fiber thickness, and cytoplasmic membrane roughness, with significant increase in Runx2 mRNA levels. These results show that bone marrow stem cells demonstrate similar cytoskeletal adaptations to low-magnitude high-frequency mechanical loads both during quiescence and osteogenesis, potentially becoming more sensitive to additional loads by increased structural stiffness.

show abstract

“…Our previous studies have shown that diamagnetic levitation caused by large gradient high superconducting magnet influences cytoskeleton arrangement and expression of associated genes in osteoblast-like and osteocyte-like cells (Qian et al 2008(Qian et al , 2009(Qian et al , 2010. In this study, 48 h of clinorotation, but not 12 h and 24 h of clinorotation, destroyed stress fibre formation and α-tubulin distribution in MLO-Y4 cells.…”

Section: Discussionmentioning

confidence: 69%

Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells

Weng

et al. 2012

Biologia

View full text Add to dashboard Cite

The aim of this study was to investigate the effects of the clinostat-simulated weightlessness on biological characteristics of MLO-Y4 osteocyte-like cells. MLO-Y4 cells were incubated for 24 h, then randomly divided into 3 groups and rotated in a clinostat as a model of simulated weightlessness for 12 h, 24 h and 48 h. The morphology, cytoskeleton, and secretion of soluble molecules of MLO-Y4 cells were observed and detected. The results show that clinostat culture affects the number of dendrites/cell, cytoskeleton distribution, and secretion of nitric oxide and prostaglandin E2 in MLO-Y4 cells. These results may provide some clue to explore the cellular mechanism of bone loss caused by weightlessness.

show abstract

High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

Cited by 27 publications

References 49 publications

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells

Bone marrow stem cells adapt to low-magnitude vibrations by altering theircytoskeleton during quiescence and osteogenesis

Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells

Contact Info

Product

Resources

About