“…For example, IVM demonstrated that fluorescently labelled bisphosphonates, which have been shown to possess anti-tumour activity, were taken up by tumour-associated macrophages (TAMs), potentially corrupting their tumourpromoting functions (Junankar et al, 2015). Here IVM identified Imaging of fibrillar collagen in tissues including muscle, skin and tumours (Brown et al, 2003;Campagnola et al, 2002;Koenig and Riemann, 2003;Kular et al, 2015;Plotnikov et al, 2006;Zipfel et al, 2003) Imaging of fibrillar collagen in human breast tissue in relation to mammographic density (Huo et al, 2015), during cancer cell invasion (Andrlová et al, 2017) or anti-cancer drug targeting of the stromal ECM (Chang et al, 2017;Miller et al, 2015;Vennin et al, 2017) Third harmonic generation (THG) Excitation primarily occurs at water-lipid and water-protein interfaces, to provide structural information (e.g. cell morphology and surrounding ECM).…”