High molecular weight forms of basic fibroblast growth factor recognized by a new anti‐bFGF antibody

Grothe, Claudia; Zachmann, Karolin; Unsicker, Klaus; Westermann, Reiner

doi:10.1016/0014-5793(90)80059-r

Cited by 36 publications

(26 citation statements)

References 21 publications

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“…In the growing postnatal testis of the rat, the Sertoli cell apparently is able to produce a bFGF-related factor, as concluded from in vitro experiments with isolated Sertoli cells from immature rats (4,7). In the present study we have shown expression of a 30-kDa bFGF-like protein in germ cells of the adult testes of two rodent species, rat and mouse, and, in addition, in the immature rat testis, using a recently characterized, highly specific antiserum against bFGF (8,9).…”

supporting

confidence: 72%

“…Several higher mol wt forms (ranging from 20-25 kDa) have been described so far (13)(14)(15), and a number of less well characterized bFGFimmunoreactive proteins with higher molecular masses (including 24-, 27-, 29-, 30-, and 46-kDa forms) have been reported by various groups (8,9,(16)(17)(18). Interestingly, a 29-kDa form of bFGF was the predominant form in the pituitary and various brain regions of the rat (17).…”

Section: Discussionmentioning

confidence: 91%

“…The 30-kDa bFGF-like protein found in rodent testes could represent such a precursor. This is not only indicated by the fact that it was recognized by a well characterized antibody, which reacts with various forms of bFGF, including 18-, 24-, 30-, and 46-kDa forms, that were found in other tissues (adrenal, ovary, and pituitary) (8,9), but also by the fact that preabsorbtion of the antiserum with recombinant bFGF abolished the specific staining.…”

Section: Discussionmentioning

confidence: 96%

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Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

et al. 1991

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supporting

confidence: 72%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 96%

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Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

et al. 1991

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“…Bohlen and Baird), and has been frequently characterized (see Blottner et al, 1989;Gonzalez et al, 1990;Grothe et al, 1990;1991b Specificity controls were carried out by 1) adsorbing the antibody to an excess of purified human recombinant FGF-2 (generous gift from PROGEN, Heidelberg, Federal Republic of Germany), 2) omitting the first antibody, or 3) replacing it with normal rabbit serum (cf. Grothe et al, 1991b).…”

Section: Methodsmentioning

confidence: 99%

Fibroblast growth factor‐2‐like immunoreactivity in auditory brainstem nuclei of the developing and adult rat: Correlation with onset and loss of hearing

Riedel

Friauf

Grothe

et al. 1995

J of Comparative Neurology

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Fibroblast growth factor-2 (FGF-2; basic FGF) is widely distributed in the developing and adult brain and has numerous effects on cultured and lesioned neural cells. The physiological role of FGF-2 in the unlesioned nervous system, however, is still not understood. We have studied the distribution of FGF-2 in the developing, adult, and functionally impaired central auditory system of the rat using specific antibodies and peroxidase-antiperoxidase immunocytochemistry. FGF-2-like immunoreactivity (FGF-2-IR) occurred in neuronal cell bodies and/or nerve fibers but was very rarely observed in glial cells. Several auditory brainstem nuclei, including the superior paraolivary nucleus, the medial superior olive, the lateral and ventral trapezoid nuclei, and the central nucleus, as well as the external cortex of the inferior colliculus, were entirely devoid of FGF-2-IR. In the dorsal cochlear nucleus, the lateral superior olive, and the nuclei of the lateral lemniscus, FGF-2-IR was not detectable in nerve cell bodies prior to adult age. Neurons in the medial geniculate body exhibited FGF-2-IR only transiently, from postnatal day (P) 5 until P16. Neurons in the medial nucleus of the trapezoid body were immunoreactive from P8 onwards. FGF-2-IR in anteroventral and posteroventral cochlear neurons disappeared at P14, i.e., at the onset of hearing, but immunoreactivity returned after P21. A transient expression of FGF-2 around the time when hearing function commences was observed in the dorsal cortex of the inferior colliculus.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The characteristics of the polyclonal anti-FGF-2 antibody were described earlier (Grothe et al 1990). In brief, the antiserum was generated in rabbits immunised against purified bovine pituitary FGF-2.…”

Section: Antibodiesmentioning

confidence: 99%

Localization of fibroblast growth factor 2 (FGF-2) protein and the receptors FGFR 1-4 in normal human seminiferous epithelium

Steger

Tetens

Seitz

et al. 1998

Histochemistry and Cell Biology

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Fibroblast growth factor 2 (FGF-2), which occurs in various isoforms both species and tissue specifically, regulates cell proliferation and differentiation via a dual receptor system consisting of heparan sulphate proteoglycans and receptor tyrosine kinases (FGFRs). This study demonstrates for the first time the distribution pattern of FGF-2 and the receptors FGFR 1-4 in the normal seminiferous epithelium of adult men. In western blot analyses, the polyclonal antibody, anti-FGF-2, shows two immunoreactive bands at 18 and 24 kDa. On paraffin sections, positive immunoreaction occurs within the cytoplasm of spermatogonia. The distribution pattern of the polyclonal anti-FGFR 1-4 antibodies is as follows: anti-FGFR-1 (one 68-kDa band) stains nuclei and cytoplasm of spermatogonia; anti-FGFR-3 (five bands at 68, 78, 105, 125 and 145 kDa) stains the nuclei of all germ cells except those of elongated spermatids; and anti-FGFR-4 (one 48-kDa band) stains the cytoplasm of primary pachytene spermatocytes. We were unable to demonstrate FGFR-2 immunoreactivity either in western blot analysis or on paraffin sections. This distribution pattern suggests that FGF-2 in spermatogonia is involved in the autocrine and paracrine regulation of the proliferation and differentiation of spermatogonia and spermatocytes via the receptors FGFR-1, FGFR-3 and FGFR-4.

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High molecular weight forms of basic fibroblast growth factor recognized by a new anti‐bFGF antibody

Cited by 36 publications

References 21 publications

Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

Presence and Localization of a 30-kDa Basic Fibroblast Growth Factor-Like Protein in Rodent Testes*

Fibroblast growth factor‐2‐like immunoreactivity in auditory brainstem nuclei of the developing and adult rat: Correlation with onset and loss of hearing

Localization of fibroblast growth factor 2 (FGF-2) protein and the receptors FGFR 1-4 in normal human seminiferous epithelium

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