High molecular weight multimer form of adiponectin as a useful marker to evaluate insulin resistance and metabolic syndrome in Japanese men

Seino, Y.; Hara, Hiroshi; Saito, Ikuo; Itoh, Hiroshi

doi:10.1016/j.metabol.2007.06.015

Cited by 95 publications

(71 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plasma glucose, serum low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, triglycerides, and uric acid levels were assayed by routine automated laboratory methods, as described previously. [16][17][18] HMW-adiponectin was measured using a commercially available kit (HMW Adiponectin ELISA Kit, Fujirebio Inc., Tokyo). This ELISA system does not need a denaturing step, and the monoclonal antibody (IH7) is reported to react specifically with the HMW form of adiponectin.…”

Section: Vol 49 Nomentioning

confidence: 99%

Effects of 12-Month Valsartan Therapy on Glycation and Oxidative Stress Markers in Type 2 Diabetic Subjects With Hypertension

et al. 2008

Self Cite

View full text Add to dashboard Cite

SUMMARYAlthough it has been reported that angiotensin II receptor blockers inhibited the formation and accumulation of advanced glycation endproducts (AGEs) in vitro and in vivo, whether they can do so clinically is not clear. We investigated the effects of 12-month valsartan therapy on various markers of inflammation, glycation, and oxidation in type 2 diabetic subjects with hypertension.We started 40 mg/day valsartan treatment in 15 type 2 diabetic patients with hypertension. In 6 patients, the dose of valsartan was increased to 80 mg/day after 6 months and maintained until 12 months. Metabolic parameters including BMI and serum high molecular weight (HMW)-adiponectin, high-sensitivity C-reactive protein (hs-CRP) as an inflammation marker, AGEs, paraoxonase activity, platelet-activating factor (PAF)-acetylhydrolase activity, and urine 8-isoprostane levels were measured at baseline and after 6 and 12 months of treatment. Urine microalbumin level and carotid artery intimamedia thickness (IMT) were also measured.Even after valsartan therapy, the blood pressure levels of the patients were not decreased significantly. Serum AGEs and urine 8-isoprostane levels decreased at both 6 and 12 months (P < 0.05 for both), although other metabolic and oxidative markers were unchanged. Though urine microalbumin levels tended to be decreased after 6 and 12 months of valsartan treatment, the changes were not significant. Mean IMT at 12 months was not changed from the baseline value. In conclusion, the findings suggest that treatment with valsartan, even at a low dose, may ameliorate some glycation and oxidative stress markers independently of an effect on blood pressure in hypertensive type 2 diabetic subjects. (Int Heart J 2008; 49: 681-689)

show abstract

Section: Vol 49 Nomentioning

confidence: 99%

Effects of 12-Month Valsartan Therapy on Glycation and Oxidative Stress Markers in Type 2 Diabetic Subjects With Hypertension

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…17,18 Plasma levels of the HMW isoform and the ratio of HMW to total adiponectin are more closely associated with glucose tolerance and insulin sensitivity than total adiponectin. 19,20 Taking into account the selective decreased levels of circulating HMW adiponectin in individuals with obesity 21,22 and the different endocrine activity of SAT and VAT, 23 the fat depot-specific alterations of adiponectin isoforms production may be expected.…”

Section: Introductionmentioning

confidence: 99%

The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue

et al. 2011

View full text Add to dashboard Cite

OBJECTIVE: Hypoadiponectinemia observed in obesity is associated with insulin resistance, diabetes and atherosclerosis. The aim of the present study was to investigate secretion of adiponectin and its multimeric isoforms by explants derived from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in obese and non-obese subjects. DESIGN: Paired samples of SAT and VAT and blood samples were obtained from 23 subjects (10 non-obese and 13 obese) undergoing elective abdominal surgery. Total adiponectin quantities and adiponectin isoforms were measured in conditioned media of explants derived from SAT and VAT using enzyme-linked immunosorbent assay and non-denaturing western blot, respectively. RESULTS: Total adiponectin plasma levels were lower in obese than in non-obese subjects (Po0.05). Secretion of total adiponectin in adipose tissue (AT) explants was lower in obese than in non-obese subjects in SAT (Po0.05) but not in VAT. In both, SAT and VAT, the most abundant isoform released into conditioned media was the high-molecular weight (HMW) form. Its relative proportion in relation to total adiponectin was higher in conditioned media of explants from both fat depots when compared with plasma (Po0.001). The proportion of secreted HMW vs total adiponectin was higher in VAT than in SAT explants in the group of non-obese individuals (49.3 ± 3.1% in VAT vs 40.6 ± 2.8% in SAT; Po0.01), whereas no difference between the two depots was found in obese subjects (46.2 ± 3.0 % in VAT vs 46.0 ± 2.4 % in SAT). CONCLUSION: Obesity is associated with the decrease of total adiponectin secretion in SAT. The profile of adiponectin isoforms secreted by SAT and VAT explants differs from that in plasma. Secretion of total adiponectin and HMW isoform of adiponectin are different in obese and non-obese subjects in relation to AT depot.

show abstract

“…In particular, the high-molecularweight (HMW) form of adiponectin has been proposed to be an active form [18][19][20] . We and other groups have shown that HMW adiponectin levels are more strongly correlated with MetS and CVD than total adiponectin levels 13,21,22) . Basu et al have also shown that HMW, but not low-molecular-weight, adiponectin is selectively decreased in individuals with type 2 diabetes, suggesting that HMW adiponectin plays an important role in the development of CVD 23) .…”

mentioning

confidence: 66%

Usefulness of C-Reactive Protein to High-Molecular-Weight Adiponectin Ratio to Predict Insulin Resistance and Metabolic Syndrome in Japanese Men

Saisho

Hara

Seino

et al. 2010

JAT

Self Cite

View full text Add to dashboard Cite

Aim:We questioned whether the ratio of C-reactive protein to high-molecular-weight adiponectin (C/A ratio), compared to each value alone, is more useful to predict insulin resistance and/or metabolic syndrome. Methods: We measured serum CRP and HMW adiponectin levels in 841 Japanese men who had participated in an annual health checkup. Correlations of the C/A ratio with metabolic parameters were assessed, and its predictive values for insulin resistance and MetS were compared with CRP or HMW adiponectin alone. Results: The C/A ratio was higher in subjects with MetS (n 114) than in those without MetS (0.46 0.67 vs. 0.23 0.39, p 0.0001). The C/A ratio was correlated with a larger number of metabolic parameters than CRP, but the correlation was comparable to HMW adiponectin. Likewise, the area under the curve of the C/A ratio in receiver operator characteristic analysis for MetS was greater than that of CRP, but comparable to that of HMW adiponectin. However, the AUC of the C/A ratio in ROC analysis for insulin resistance (HOMA-IR 2.5) was greater than that of CRP or HMW adiponectin alone. Conclusion: While the C/A ratio provided little advantage to predict MetS, it might be more useful to predict insulin resistance than CRP or HMW adiponectin alone. J Atheroscler Thromb, 2010; 17:944-952.

show abstract

High molecular weight multimer form of adiponectin as a useful marker to evaluate insulin resistance and metabolic syndrome in Japanese men

Cited by 95 publications

References 36 publications

Effects of 12-Month Valsartan Therapy on Glycation and Oxidative Stress Markers in Type 2 Diabetic Subjects With Hypertension

Effects of 12-Month Valsartan Therapy on Glycation and Oxidative Stress Markers in Type 2 Diabetic Subjects With Hypertension

The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue

Usefulness of C-Reactive Protein to High-Molecular-Weight Adiponectin Ratio to Predict Insulin Resistance and Metabolic Syndrome in Japanese Men

Contact Info

Product

Resources

About