High number of CD56bright NK-cells and persistently low CD4+ T-cells in a hemophiliac HIV/HCV co-infected patient without opportunistic infections

Fregni, Giulia; Maresca, A; Jalbert, Valérie; Caignard, Anne; Scott‐Algara, Daniel; Cramer, Elisabeth Bordé; Rouveix, É.; Béné, Marie C.; Capron, Claude

doi:10.1186/1743-422x-10-33

Cited by 7 publications

(4 citation statements)

References 32 publications

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“… 37 An asymptomatic hemophiliac patient co-infected with HIV/HCV also had increased CD56 bri CD16 − NK cells. 38 These results suggest that the CD56 bri CD16 − regulatory NK cells may play a critical role in protecting SARS-CoV-2 infection in asymptomatic patients.…”

Section: Discussionmentioning

confidence: 78%

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Zhao

You

Cui

et al. 2021

Sig Transduct Target Ther

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While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56briCD16− natural killer (NK) cells and upregulation of interferon-gamma in effector CD4+ and CD8+ T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4+ T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.

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Section: Discussionmentioning

confidence: 78%

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Zhao

You

Cui

et al. 2021

Sig Transduct Target Ther

View full text Add to dashboard Cite

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“…Virus induced NK cell hyperresponsiveness and cytokine production, particularly in the CD56 bri CD16 -NK subset (Scharenberg et al, 2019). An asymptomatic hemophiliac patient coinfected with HIV/HCV also had increased CD56 bri CD16 -NK cells (Fregni et al, 2013). These results suggested that the CD56 bri CD16regulatory NK cells may play a critical role in the protection of SARS-CoV-2 infection in asymptomatic patients.…”

Section: Cd56 Bri Cd16 -Nk Cells Have Been Linked To Virus Infectionmentioning

confidence: 79%

Longitudinal single-cell immune profiling revealed distinct innate immune response in asymptomatic COVID-19 patients

Zhao

You

Wang

et al. 2020

Preprint

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SUMMARYRecent studies have characterized the single-cell immune landscape of host immune response of coronavirus disease 2019 (COVID-19), specifically focus on the severe condition. However, the immune response in mild or even asymptomatic patients remains unclear. Here, we performed longitudinal single-cell transcriptome sequencing and T cell/B cell receptor sequencing on 3 healthy donors and 10 COVID-19 patients with asymptomatic, moderate, and severe conditions. We found asymptomatic patients displayed distinct innate immune responses, including increased CD56briCD16− NK subset, which was nearly missing in severe condition and enrichment of a new Th2-like cell type/state expressing a ciliated cell marker. Unlike that in moderate condition, asymptomatic patients lacked clonal expansion of effector CD8+ T cells but had a robust effector CD4+ T cell clonal expansion, coincide with previously detected SARS-CoV-2-reactive CD4+ T cells in unexposed individuals. Moreover, NK and effector T cells in asymptomatic patients have upregulated cytokine related genes, such as IFNG and XCL2. Our data suggest early innate immune response and type I immunity may contribute to the asymptomatic phenotype in COVID-19 disease, which could in turn deepen our understanding of severe COVID-19 and guide early prediction and therapeutics.

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“…Specific KIR and HLA genotype combinations influence rates of HIV-1 acquisition and disease progression, perhaps due to KIR-mediated NK cell licencing or recognition of HIV-1-derived peptides by non-clonotypic receptors. Interestingly, high percentage of CD56 hi NK cells has been associated with nearly undetectable viral load 50 , suggesting that TCF7 + NKG2C + CD94 + CD56 hi NK cells can control HIV-1. Better understanding of these memory NK cells is hoped to lead to new approaches for preventing and controlling HIV-1 and other infections.…”

Section: Discussionmentioning

confidence: 99%

HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells

et al. 2020

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HIV-1 infection is associated with heightened inflammation and excess risk of cardiovascular disease, cancer, and other complications. These pathologies persist despite antiretroviral therapy (ART). In two independent cohorts, we found that innate lymphoid cells (ILCs) were depleted in the blood and gut of people with HIV-1, even with effective ART. ILC depletion was associated with neutrophil infiltration of the gut lamina propria, type 1 interferon activation, increased microbial translocation, and natural killer (NK) cell skewing towards an inflammatory state with chromatin structure and phenotype typical of WNT transcription factor TCF7-dependent memory T cells. Cytokines that are elevated during acute HIV-1 infection reproduced the ILC and NK cell abnormalities ex vivo . These results demonstrate that inflammatory cytokines associated with HIV-1 infection irreversibly disrupt ILCs. This results in loss of gut epithelial integrity, microbial translocation, and memory NK cells with heightened inflammatory potential, and explains the chronic inflammation in people with HIV-1.

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High number of CD56bright NK-cells and persistently low CD4+ T-cells in a hemophiliac HIV/HCV co-infected patient without opportunistic infections

Cited by 7 publications

References 32 publications

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Longitudinal single-cell immune profiling revealed distinct innate immune response in asymptomatic COVID-19 patients

HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells

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