High Passage Number of Stem Cells Adversely Affects Stem Cell Activation and Myocardial Protection

Crisostomo, Paul R.; Wang, Meijing; Wairiuko, George M.; Morrell, Eric D.; Terrell, Andrew M.; Seshadri, Preethi; Nam, Un Hui; Meldrum, Daniel R.

doi:10.1097/01.shk.0000235087.45798.93

Cited by 156 publications

(113 citation statements)

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“…It has been suggested that with increasing time in cell culture, and with increasing passages, cultured MSCs lose their proliferation and differentiation potential, and senesce [32,33]. As the cell passage number used in the present study was low, this was not one of the causes of treatment failure.…”

Section: Disccusionmentioning

confidence: 75%

Intravenous administration of adipose-derived stromal cells does not ameliorate bleomycin-induced lung injury in rats

Uji¹,

Nakada²,

Nakamura³

2013

OJRM

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Background: Mesenchymal stromal cells (MSCs) have been studied intensively in regenerative medicine. Among MSCs, adipose tissue-derived stromal cells (ASCs) are relatively easy to obtain from a patient. Since ASCs are ideal candidates for use in the treatment of disease states including pulmonary fibrosis, we investigated whether intravenous injection of ASCs could exert a therapeutic effect against bleomycininduced lung injury in rats. Methods: Rats were intratracheally administered bleomycin, and one week later ASCs were isolated and cultured. Two weeks after bleomycin treatment ASCs or PBS (phosphate-buffered saline) were injected to the rats. Three or six weeks after bleomycin instillation, the total cell counts and their profile in bronchoalveolar lavage fluid (BALF) were measured, and a histological evaluation was semiquantitatively assessed for the injured lungs, followed by cell tracing. Results: The BALF cell counts and its profiles were not significantly different in the ASCs and PBS groups. Furthermore, ASC treatment led to no significant histological effect compared with the PBS treatment. Using a fluorescent cell tracer, it was noted that the ASCs homed to the injured lung areas, but some ASCs accumulated around scars, and scarcely migrated into the fibrotic areas. Conclusions: In the present study, the intravenous administration of ASCs could not reduce the severity of bleomycin-induced lung injury in a rat model. Although the ASC counts and passage numbers were suitable, the older age and fibrotic disease stage of the rats were likely responsible for the treatment failure.

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Section: Disccusionmentioning

confidence: 75%

Intravenous administration of adipose-derived stromal cells does not ameliorate bleomycin-induced lung injury in rats

Uji¹,

Nakada²,

Nakamura³

2013

OJRM

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“…They can also play an important role in wound healing, organ donation and chronic immunesuppressive therapies (Pittenger et al 1999;Barry and Murphy 2004;Phinney and Prockop 2007;Prockop 1997). However, the problems of the low induction efficiency and high risk of the stem cells in clinical application have not been addressed yet (Colter et al 2000;Crisostomo et al 2006). Therefore, the development of a method that can produce higher-quality stem cells with improved differentiation potential is highly significant.…”

Section: Introductionmentioning

confidence: 99%

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Wang

Zhang

et al. 2013

Cytotechnology

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Multi-differentiation capability is an essential characteristic of bone marrow mesenchymal stem cells (BMSCs). Method on obtaining higherquality stem cells with an improved differentiation potential has gained significant attention for the treatment of clinical diseases and developmental biology. In our study, we investigated the multipotential differentiation capacity of BMSCs under simulated microgravity (SMG) condition. F-actin staining found that cytoskeleton took on a time-dependent change under SMG condition, which caused spindle to round morphological change of the cultured cells. Quantitative PCR and Western Blotting showed the pluripotency marker OCT4 was up-regulated in the SMG condition especially after SMG of 72 h, which we observed would be the most appropriate SMG duration for enhancing pluripotency of BMSCs. After dividing BMSCs into normal gravity (NG) group and SMG group, we induced them respectively in endothelium oriented, adipogenic and neuronal induction media. Immunostaining and Western Blotting found that endothelium oriented differentiated BMSCs expressed higher VWF and CD31 in the SMG group than in the NG group. The neuron-like cells derived from BMSCs in the SMG group also expressed higher level of MAP2 and NF-H. Furthermore, the quantity of induced adipocytes increased in the SMG group compared to the NG group shown by Oil Red O staining, The expression of PPARc2 increased significantly under SMG condition. Therefore, we demonstrated that SMG could promote BMSCs to differentiate into many kinds of cells and predicted that enhanced multi-potential differentiation capacity response in BMSCs following Electronic supplementary material The online version of this article

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“…These authors have also observed that these cells maintain the same physiology until five passages 23,24 .…”

Section: Discussionmentioning

confidence: 69%

“…Is important to note that the second passage also increase the quantity of MSC by 164% (on relation of CTF) showing that these cells have a good capacity of proliferation, like other authors have observed [20][21][22][23][24] . These authors have also observed that these cells maintain the same physiology until five passages 23,24 .…”

Section: Discussionmentioning

confidence: 87%

Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells

et al. 2010

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Purpose:To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering. Methods: New Zealand rabbits (n= 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles. Results: The fat total cells (CTF) were 1.62 x10 6 cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10 6 cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10 6 cells/mL MSC. Conclusion: The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively. Key words: Lipectomy. Stem Cells. Fats. Rabbits. RESUMOObjetivo: Apresentar um modelo experimental de análise qualitativa e quantitativa de células tronco mesênquimais proveniente da gordura de coelhos obtido por lipectomia. A gordura poderia ser uma grande fonte de obtenção de células tronco mesenquimais, criando condições para a reparação de tecidos lesados. Métodos: Foram removidos os panículos adiposos (2-3 cm) da região cervical de Coelhos Nova Zelândia (n = 10) por lipectomia. Os panículos foram fragmentados e lavados com PBS e, posteriormente, dissociados enzimaticamente com tripsina / EDTA. As células extraídas do panículo adiposo foram incubadas em meio de cultura DMEM e após 20 dias, foi realizada uma análise quantitativa da adesão de primeira e segunda passagem das células mesênquimais em garrafas de cultura. Resultados: Foram extraídas 1,62 x106 cel/ mL células totais de gordura (CTG) with 98% de viabilidade. Essas células foram levadas para o cultivo e após 20 dias, foi realizada a primeira passagem (1pd) sendo quantificadas 2,88 x10 6 cel/mL células tronco mesênquimais (CTM). Na segunda passagem (2pd) foi obtido 4,28 x10 6 cel/mL CTM. Conclusão: A lipectomia do paniculo adiposo é um método muito satisfatório para extrair células tronco a partir de gordura, quantitativamente e qualitativamente. Descritores: Lipectomia. Células-Tronco. Gorduras. Coelhos.

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High Passage Number of Stem Cells Adversely Affects Stem Cell Activation and Myocardial Protection

Cited by 156 publications

References 50 publications

Intravenous administration of adipose-derived stromal cells does not ameliorate bleomycin-induced lung injury in rats

Intravenous administration of adipose-derived stromal cells does not ameliorate bleomycin-induced lung injury in rats

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells

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