High-performance anion-exchange chromatography of organic acids

Vrátný, P.; Mikeš, O.; Štrop, Petr; Čoupek, J.; Rexová-Benková, Ljuba; Chadimová, Dana

doi:10.1016/s0021-9673(01)88149-8

Cited by 22 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several methods have been proposed in the literature for the determination of organic acids in various plant parts and foods, for example, reverse-phase high-performance liquid chromatography (RP-HPLC) (4-8), ion-exchange chromatography (9)(10)(11)(12)(13), and capillary electrophoresis (6,14). The determination of organic acids in the stem exudate of tomato and soybean grown in normal and in Zn-phytotoxic nutrient solutions was performed using a gas-liquid chromatography technique (GLC) by White et al (15).…”

Section: Introductionmentioning

confidence: 99%

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Tatár

Mihucz

Varga

et al. 1998

Microchemical Journal

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Tatár

Mihucz

Varga

et al. 1998

Microchemical Journal

View full text Add to dashboard Cite

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC). III. Quantitative analysis of organic acids in the α‐ketoglutaric acid synthesis

Gey¹,

Weissbrodt²,

Stottmeister³

1988

Acta Biotechnologica

View full text Add to dashboard Cite

A HPLC technique for the ancllys,is of organic acids in the production of a-ketoglutaric acid was developed. The method was applied and optimized for the quantitative determination of citric acid, pyruvic acid, iaocitric acid and a-ketoglutoric acid in fermentation solutions.As microorganism the yeast Yarroroia lipolytk and as substrates glucose or paraffins were used. The chromatographic separations were carried out by means of 50 and 1 0 0 x 8 m m i.d. glass columns packed with an anion-exchange resin based on an 8% cross-linked polystyrene-divinylbenzene copolymer. The relative errors ranged from 2.1% (a-ketoglutaric acid) to 5.2% (isocitric acid). The percent recovery values varied between 94.4% (isocitric acid) and 107.7% (pyruvic acid). The contents of organic acids in fermentation solutions after the microbial synthesis based on paraffins or glucose were compared.

show abstract

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC) VI. Determination of lactic acid and short‐chain carboxylic acids C₁–C₅

Gey

Klossek

Becker

1990

Acta Biotechnologica

View full text Add to dashboard Cite

Lactic acid and short-chain carboxylic acids C,-C, were analyzed on a n Aminex HPX-87H column using UV and RI detection. The HPLC method was applied to complex fermentation media of a microbial lactic acid formation process. The resulting chromatograms of the yeast extract or culture medium showed some W-absorbing substances that interfered partially with peaks of shortchain carboxylic acids. I n contrast t o UV monitoring, R I detection gave only a few small peaks of these so-called "background chromatograms". Also glucose used as substrate could be quantified in the culture medium, because of which R I detection should be preferred. Furthermore, a self-prepared cation-exchange resin (SAC = S-DVB) based on a poly(styrenedivinylbenzene) partially comparable in its properties with Aminex resins was useful for fast (about 4 minutes) and not too expensive determinations of lactic and acetic acid in fermentation media. Applying this separation system to fermentation solutions detected b y R I as well as UV monitoring results in the quantitative analysis of lactic acid kinetics identical with a n Aminex HPX-87H column were achieved. Finally, the simplicity of lactic-acid analysis is illustrated by examples of dairy products only centrifugated and diluted in double distilled water before injecting on a cation-exchange column.

show abstract

High-performance anion-exchange chromatography of organic acids

Cited by 22 publications

References 22 publications

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC). III. Quantitative analysis of organic acids in the α‐ketoglutaric acid synthesis

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC) VI. Determination of lactic acid and short‐chain carboxylic acids C₁–C₅

Contact Info

Product

Resources

About

High-performance anion-exchange chromatography of organic acids

Cited by 22 publications

References 22 publications

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Determination of Organic Acids in Xylem Sap of Cucumber: Effect of Lead Contamination

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC). III. Quantitative analysis of organic acids in the α‐ketoglutaric acid synthesis

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC) VI. Determination of lactic acid and short‐chain carboxylic acids C1–C5

Contact Info

Product

Resources

About

Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC) VI. Determination of lactic acid and short‐chain carboxylic acids C₁–C₅