High-performance bioanalysis based on ion concentration polarization of micro-/nanofluidic devices

Wang, Chen; Wang, Yang; Zhou, Yue; Wu, Zeng-Qiang; Xia, Xing‐Hua

doi:10.1007/s00216-019-01756-8

Cited by 29 publications

(22 citation statements)

References 78 publications

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“…In this context, developing new solutions for sample analyte preconcentration in bioanalytical fluidic devices remains a necessity. To address this prevalent issue, many focusing techniques based on electrokinetic phenomena have been proposed, among which ion concentration polarization [8‐20], field‐amplified sample stacking (FASS) [21‐23], concentration gradient focusing [24‐27], and isoelectric focusing [28‐30]. All these techniques allow focusing analytes by exploiting the unbalanced ionic transport between anionic and cationic species due to the competition between electroosmotic flow (EOF) and electrophoretic flow (EP).…”

Section: Introductionmentioning

confidence: 99%

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

et al. 2020

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Concentration polarization (CP)-based focusing electrokinetics nanofluidic devices have been developed in order to simultaneously detect and enrich highly diluted analytes ona-chip. However, stabilization of focal points over long time under the application of the electric field remains as a technical bottleneck. If pressure-assisted preconcentration methods have been proposed to stabilize propagating modes at low inverse Dukhin number (1/D u 1), these recent protocols remain laborious for optimizing experimental parameters. In this paper, "electric field E/counter-pressure P" diagrams have been established during pressure-assisted electro-preconcentration of fluorescein as a model molecule. Such E/P diagram allows direct observation of the region for which the optimal counterpressure P leads to a stable focusing regime. This region of stable focusing is shown to vary depending of the nanoslit length (100 μm < L nanoslit < 500 μm) and the nature of the background electrolyte (KCl and NaCl). Longer nanoslits (500 μm) produce stabilization at low counter-pressure P, whereas NaCl offers a narrower region of stable focusing in the E/P diagram compared to KCl. Finally, the ability of such pressure-assisted protocol to concentrate negatively charged proteins has been tested with a more applicative protein, i.e., ovalbumin. The corresponding E/P diagram confirms the existence of the stable focusing regime at both low electric field E (≤20 V) and counter-pressure P (≤0.4 bar). With an enrichment factor as high as 70 after 2 min for ovalbumin at a concentration of 10 μM, such pressure-assisted nanofluidic electro-preconcentration protocol appears very promising to concentrate and detect biomolecules.

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Section: Introductionmentioning

confidence: 99%

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

et al. 2020

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“…ICP is an interfacial phenomenon. It occurs mainly at the micro‐nano interface of the surface‐charged channel of the electric field polarization . It mainly includes two basic phenomena: the ion depletion effect and the ion enrichment effect .…”

Section: Introductionmentioning

confidence: 99%

A review: applications of ion transport in micro‐nanofluidic systems based on ion concentration polarization

Han

Chen

2019

J of Chemical Tech & Biotech

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Lab-on-a-chip has been used widely in rapid, high-throughput and low-consumption analysis of samples in biochemistry. The ion concentration polarization (ICP) produced by ion-selective transport of nanochannels provides a novel solution for problems in ultra-low concentration sample detection, systems biology and desalination. This paper reviews the applications of ion transport based on the principle of ICP in micro-nanofluidic systems. First, the fundamental governing equations of ICP are described. Then, the applications of nano-electrokinetic ion enrichment and ion current rectification (ICR) are introduced. Nano-electrokinetic ion enrichment is used mainly in the fields of molecular enrichment, ultra-low concentration sample detection and seawater desalination. ICR is applied mainly to the sensitive detection of analytical substances such as proteins, nucleic acids and small molecules. The application of ion transport based on ICP principle is summarized and the possible directions worthy of further research are proposed.

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“…[351] While this work provides a theoretical modeling analysis, others have put these principles to the test in ion concentration polarization devices. [352,353] Furthermore, preconcentrators can be used to reduce the negative effects of electroosmotic flow on ion enrichment. [354] Massively parallel hierarchical nanochannels can concentrate nucleic acids, proteins, and bacteria, at concentrations down to the attomolar level, in complex samples including whole blood.…”

Section: Nanochannelsmentioning

confidence: 99%

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

Welch

Powell

Clevinger

et al. 2021

Adv Funct Materials

115

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Nanoscale materials have unique properties that make them especially useful for biomedical diagnostic applications. Recent developments in nanoengineering have resulted in increasing use of nanostructures in biosensors. Various types of 0D, 1D, 2D, and 3D nanostructures have been used to improve biosensor sensitivity, selectivity, limit of detection, and time to result, among other metrics. These nanostructures have been integrated into electrochemical, optical, and other biosensors for this purpose. Here, the most recent advances in the use of nanostructured materials in biosensors are described. This includes a discussion of nanoparticles, nanorods, nanofibers, nanopillars, nanowires, nanosheets, indented nanopatterns (nanoholes and nanoslits), nanogaps, nanochannels, nanopores, nanofunctionalized surfaces, and complex hierarchical structures and their unique advantages and applications in biosensors. Clinical applications of these nanobiosensors are also highlighted along with a discussion of the future directions for these materials in diagnostics.

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High-performance bioanalysis based on ion concentration polarization of micro-/nanofluidic devices

Cited by 29 publications

References 78 publications

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

A review: applications of ion transport in micro‐nanofluidic systems based on ion concentration polarization

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

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