High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Radzi, Salina Mat; Basri, Mahiran; Salleh, Abu Bakar; Ariff, Arbakariya; Mohammad, Rosfarizan; Rahman, Mohd Basyaruddin Abdul; Rahman, Raja Noor Zaliha Raja Abdul

doi:10.2225/vol8-issue3-fulltext-4

Cited by 53 publications

(37 citation statements)

References 21 publications

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“…Free Fatty Acid (FFA), OAEE, ethanol, water and the conversion of ester were calculated according to Lee et al [14]; Prastowo et al [5]; Segupta and Modak [15]; Barahona et al [16], and Mat Radzi et al [17], respectively.…”

Section: Discussionmentioning

confidence: 99%

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Prastowo

Hidayat

Hastuti

2015

Bull. Chem. React. Eng. Catal.

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A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reaction. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 -95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE.

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Section: Discussionmentioning

confidence: 99%

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Prastowo

Hidayat

Hastuti

2015

Bull. Chem. React. Eng. Catal.

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“…Three types of oil phase, which are oleyl laurate, oleyl stearate and oleyl oleate, were synthesized in the laboratory using the optimized reaction conditions (5). Hydrophilic polymeric surfactant, Pluronic F68 and lipophilic surfactant, Span 20 were purchased from BASF, USA and Merck, Germany, respectively.…”

Section: Experimental Materialsmentioning

confidence: 99%

“…The three oil phases were fatty acid esters of fatty alcohols, which had been synthesized enzymatically (5). The purpose of using these fatty acid esters of fatty alcohols as the oil phase was due to their unique properties, such as their excellent wetting behaviour and without the oily feeling.…”

Section: Construction Of Pseudo-ternary Phase Diagrams Using Single Wmentioning

confidence: 99%

“…With the introduction of green chemistry, enzymatic synthesis is considered to satisfy its concept due to the environmentally friendly process with a high yield of wax ester with improving quality and purity of the product (1). The synthesis of wax esters have been carried out in mild reaction conditions, such as at normal pressure and temperatures (2)(3)(4)(5). Wax esters possess potential applications due to their excellent wetting behaviour at interfaces and a non-greasy feeling when applied on the skin surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Phase Behaviour and Formation of Fatty Acid Esters Nanoemulsions Containing Piroxicam

et al. 2013

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Abstract. Fatty acid esters are long-chain esters, produced from the reaction of fatty acids and alcohols. They possess potential applications in cosmetic and pharmaceutical formulations due to their excellent wetting behaviour at interfaces and a non-greasy feeling when applied on the skin surfaces. This preliminary work was carried out to construct pseudo-ternary phase diagrams for oleyl laurate, oleyl stearate and oleyl oleate with surfactants and piroxicam. Then, the preparation and optimization study via 'One-At-ATime Approach' were carried out to determine the optimum amount of oil, surfactants and stabilizer using low-energy emulsification method. The results revealed that multi-phase region dominated the three pseudo-ternary phase diagrams. A composition was chosen from each multi-phase region for preparing the nanoemulsions systems containing piroxicam by incorporating a hydrocolloid stabilizer. The results showed that the optimum amount (w/w) of oil for oleyl laurate nanoemulsions was 30 and 20 g (w/w) for oleyl stearate nanoemulsions and oleyl oleate nanoemulsions. For each nanoemulsions system, the amount of mixed surfactants and stabilizer needed for the emulsification to take place was found to be 10 and 0.5 g (w/w), respectively. The emulsification process via high-energy emulsification method successfully produced nano-sized range particles. The nanoemulsions systems passed the centrifugation test and freeze-thaw cycle with no phase failures, and stable for 3 months at various storage temperatures (3°C, 25°C and 45°C). The results proved that the prepared nanoemulsions system cannot be formed spontaneously, and thus, energy input was required to produce nano-sized range particles.

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“…All experiments were performed in triplicate. The amount of free acid in the reaction mixture and control was specified and the percentage of conversion was defined as the ratio of converted acid in the reaction mixture to total amount of acid used in the reaction (Equation 1) [10]. % Conversion = (1 -A/B) ×100…”

Section: Analysis and Characterizationmentioning

confidence: 99%

High Performance Enzyme-Catalyzed Synthesis and Characterization of a Nonionic Surfactant

Adnani¹

2013

IOSR-JAC

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High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Cited by 53 publications

References 21 publications

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Phase Behaviour and Formation of Fatty Acid Esters Nanoemulsions Containing Piroxicam

High Performance Enzyme-Catalyzed Synthesis and Characterization of a Nonionic Surfactant

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