High-performance Liquid chromatographic analysis of isoniazid and acetylisoniazid in biological fluids

Drug acetylation in breast cancer

“…After fully informed written consent had been obtained, a single tablet of isoniazid 200mg was given after an overnight fast and blood sampled at 3 h for measurement of the ratio of acetylisoniazid to isoniazid by high performance liquid chromatography (Hutchings et al, 1983a). Samples were stored at -70°C before assay in order to avoid breakdown of isoniazid (Hutchings et al, 1983b). Subjects with histologically proven carcinoma of the breast were included in the breast cancer group.…”

Saliva and plasma concentrations of isoniazid and acetylisoniazid in man.

“…Isoniazid and acetylisoniazid concentrations were measured by h.p.l.c. in plasma, saliva and phosphate buffer (Hutchings et al, 1983b (Figure 1). The concentrations of isoniazid in saliva tended to be higher than those found in plasma for the first 60 min in slow acetylators (Figure 2) and for the first 45 min in fast acetylators (Figure 3), thereafter attaining approximate unity.…”

Quantification of isoniazid and acetylisoniazid in rat plasma and alveolar macrophages by liquid chromatography–tandem mass spectrometry with on-line extraction

“…More advanced HPLC analytical techniques were later developed for qualitative and quantitative analysis of INH and AcINH in biological specimens. These assays used UV [22][23][24][25], fluorimetric [26,27], or electrochemical [28][29][30] methods for detection of INH and its metabolites. Although more specific, the sensitivity of these methods was not very good.…”