High–performance liquid chromatographic determination of perphenazine in plasma

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method for the analysis of perphenazine in rabbit plasma was developed. Amitriptyline HCl was used as the internal standard. The chromatography was performed using a Microsorb-CN column; the mobile phase consisted of 85:15 methanol/0.005 M ammonium acetate buffer; flow rate of 1.6 ml/min; and ultraviolet detection at 254 nm. Retention times were 10.8 and 18.1 min for perphenazine and amitriptyline HCl, respectively. The recovery of perphenazine added to plasma in the concentration range of 0.05-5 micrograms/ml was found to be greater than 80%. This procedure was used for the analysis of plasma samples collected over time following the oral administration of perphenazine tablets in rabbits.

Serotonin and dopamine receptor gene polymorphisms and the risk of extrapyramidal side effects in perphenazine-treated schizophrenic patients

“…Plasma perphenazine concentrations were determined at the Department of Clinical Pharmacology, Huddinge University Hospital, Stockholm, Sweden, by high-performance liquid chromatography, according to a previously published method (Mandal and Ace 1993), in samples taken 12±1 h after the last perphenazine dose. The limit of quantitation was 0.1 nmol/l of perphenazine.…”

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

“…Several analytical techniques have been reported in the literature involving gas chromatography [5], HPLC with coulometric detection [6] or HPLC with UV detection [6][7][8]. All these reported HPLC techniques involved, in the sample preparation, double or triple liquid-liquid extractions with organic solvent, followed by an evaporation [6,9] or solid phase extraction [8][9][10][11].…”

“…These sample preparation procedures require a lot of time, reagents and materials, and consequently they are quite expensive. Also, some of these reported techniques require electrochemical detectors, which are seldom available [9], or special chromatographic columns [7,8].…”

Development and validation of a gas chromatographic–mass spectrometric method for quantitative determination of perphenazine in rabbit plasma after sublingual administration

“…Several methods are available for determining perphenazine and other phenothiazine-type antipsychotics from human plasma, urine or oral fluid, e.g. liquid chromatography with ultraviolet [5][6][7][8], coulometric [9][10][11], fluorescence [12] or mass spectrometric detection [13], gas chromatography with electron capture [14,15], nitrogen [16,17] or mass spectrometric detection [18][19][20][21], as well as capillary electrophoresis coupled with chemiluminescence [22] or ultraviolet-visible detection [23]. Radioimmunoassays have also been applied in the determination of phenothiazines from plasma [24,25].…”