HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Pedernera, C.; Hidalgo-Ruiz, José L.; Castells, G.; Manteca, Xavier; Cristòfol, Carles

doi:10.1016/j.jchromb.2007.04.038

Cited by 12 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However they have a complex, time consuming and expensive instrumentation and some of them use a preconcentration method before the measurement. [4][5][6][7][8][9][10]16,32 The proposed system is an easy, cheap and fast analytical tool for obtaining of preliminary chemical information about perphenazine, prior the use of more complex instrumental techniques .…”

Section: Response Characteristicsmentioning

confidence: 99%

Sensitive determination of perphenazine in pharmaceuticals and human serum by flow injection chemiluminescence method using [Ru(phen)3]2+-ce(IV) system and a chemometrical optimization approach

Rezaei

Mokhtari

2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Este artigo descreve um método de quimioluminescência (CL) simples, rápido e sensível para a determinação de perfenazina usando sistema de injeção em fluxo (FI). Todas as variáveis que podem afetar a resposta do método CL foram otimizadas empregando planejamento composto central (CCD) para o planejamento experimental e metodologia de superfície de resposta (RSM) para a modelagem. A otimização usando CCD comparativamente ao método univariado mostrou uma melhora significativa na sensibilidade. Sob condições experimentais ótimas, uma ampla região linear foi obtida entre a concentração do analito e a altura do pico, dentro do intervalo de 1,2 a 1.300 ng mL -1 com coeficiente de correlação de 0,9978. O limite de detecção foi de 0,4 ng mL -1(S/N = 3) e o desvio padrão relativo para 6 repetições de uma solução contendo 70,5 ng mLfoi menor que 4%. Esse método foi aplicado com sucesso na quantificação de perfenazina em formulações farmacêuticas e em soro humano com adequadas recuperações (95,3-104,0%). A frequência analítica foi de 100 ± 5 amostras por hora.This paper describes a simple, rapid and sensitive chemiluminescence (CL) method for the determination of perphenazine by flow injection (FI) system. All variables that can affect the CL response were optimized by employing central composite design (CCD) for the experimental design and response surface methodology (RSM) for the modeling. Optimization by means of CCD method with respect to conventional single factor at a time method showed a significant improvement in the sensitivity. Under the optimum experimental conditions, a wide linear relationship between analyte concentration and peak height was obtained within the range 1.2-1,300 ng mL -1 with correlation coefficient of 0.9978. The limit of detection was 0.4 ng mL -1 (S/N = 3) and the relative standard deviation for 6 repeated measurements of a solution containing 70.5 ng mL -1 was lower than 4%. This method was successfully applied for the quantification of perphenazine in pharmaceutical formulations and human serum with good recoveries (95.3-104.0%). Sample throughput was 100 ± 5 samples per hour. Keywords: flow injection, chemiluminescence, perphenazine, central composite design IntroductionPerphenazine belongs to the phenothiazine family of drugs. Because of its neuroleptic and antidepressive actions, it is often prescribed for the treatment of psychotic patients such as schizophrenia and schizoaffective psychoses in order to decrease restlessness, aggressiveness and impulsive behavior. Also it is found to be effectual in the treatment of Parkinson's disease. 1 The monitoring of the perphenazine is important for quality assurance in pharmaceutical industry and for obtaining optimum therapeutic concentrations in body fluids to minimize the risk of toxicity. The therapeutic range and toxic threshold of perphenazine in serum were reported 4-30 and 50-100 ng mL In experimentation, we attempt to monitor the effects of certain inputs or material on the subject matter of interest. The advantages of the experimenta...

show abstract

Section: Response Characteristicsmentioning

confidence: 99%

Sensitive determination of perphenazine in pharmaceuticals and human serum by flow injection chemiluminescence method using [Ru(phen)3]2+-ce(IV) system and a chemometrical optimization approach

Rezaei

Mokhtari

2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…In most cases, separation and preconcentration of PPZ is required prior to analysis. To do this, different pre-concentration methods such as liquid liquid extraction (LLE) 4 and solid phase extraction (SPE) 11,12,14,17 have been introduced and developed. LLE is a classical method of extraction.…”

Section: Introductionmentioning

confidence: 99%

Pre-Concentration and Separation of Perphenazine Using Picric Acid Loaded α-Fe2O3 Nanoparticles: HPLC Syringe Filter as the Particles Support

Ramezani

Shekarriz

Behfar

et al. 2017

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Suspension of picric acid (PA) loaded iron oxide nanoparticles (PA-IONPs) was injected on the top of a 0.2 µm HPLC (high-performance liquid chromatography) syringe filter. The immobilized nanoparticles were used as SPE (solid phase extraction) sorbent for perphenazine separation and pre-concentration. By passing perphenazine solution through the filter, a molecular complex between perphenazine and PA was formed. Perphenazine/PA complex was eluted from the filter using dichloromethane as solvent. The perphenazine contents were determined at 254 nm. Effects of the parameters such as pH, the amount of PA-IONPs, as well as flow rate, volume, and type of eluting solvents, on the separation efficiency and pre-concentration of perphenazine were investigated. The calibration curve was linear in the range of 0.5-5 ng mL -1 (Y = 0.047X + 0.018; R 2 = 0.9952) with limit of detection of 0.16 ng mL -1. The pre-concentration and enrichment factors were 8.33 and 1.88, respectively. This method was successfully applied to the determination of perphenazine residue in tap water.

show abstract

“…Several methods are available for determining perphenazine and other phenothiazine-type antipsychotics from human plasma, urine or oral fluid, e.g. liquid chromatography with ultraviolet [5][6][7][8], coulometric [9][10][11], fluorescence [12] or mass spectrometric detection [13], gas chromatography with electron capture [14,15], nitrogen [16,17] or mass spectrometric detection [18][19][20][21], as well as capillary electrophoresis coupled with chemiluminescence [22] or ultraviolet-visible detection [23]. Radioimmunoassays have also been applied in the determination of phenothiazines from plasma [24,25].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported several sample preparation techniques for perphenazine and related substances, including liquid-liquid extraction (LLE) [8][9][10][11][14][15][16][17][18]21] and solid phase extraction (SPE) [5,6,12,13,19,20,22,23]. Being a multi-step process, LLE is often extremely time-consuming and laborious.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of a gas chromatographic–mass spectrometric method for quantitative determination of perphenazine in rabbit plasma after sublingual administration

Turunen

Lehtonen

Järvinen

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

Cited by 12 publications

References 11 publications

Sensitive determination of perphenazine in pharmaceuticals and human serum by flow injection chemiluminescence method using [Ru(phen)3]2+-ce(IV) system and a chemometrical optimization approach

Sensitive determination of perphenazine in pharmaceuticals and human serum by flow injection chemiluminescence method using [Ru(phen)3]2+-ce(IV) system and a chemometrical optimization approach

Pre-Concentration and Separation of Perphenazine Using Picric Acid Loaded α-Fe2O3 Nanoparticles: HPLC Syringe Filter as the Particles Support

Development and validation of a gas chromatographic–mass spectrometric method for quantitative determination of perphenazine in rabbit plasma after sublingual administration

Contact Info

Product

Resources

About