High-performance liquid chromatographic determination of propiverine and its N-oxide in human serum

Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

“…This method showed excellent sensitivity (0.5 ng/ml), reproducibility, specificity and speed (analysis time 20 min). Especially, the LLOQ value was five-fold lower than that obtained by Richter et al [5], and LOD was close to that obtained by LC/MS/MS method [7,8]. In conclusion, this paper describes a simple, specific and sensitive HPLC method for the determination of PPV in human plasma.…”

“…The lower limit of quantification (LLOQ) for PPV was estimated as 2 ng/ml. LLOQ value of PPV was lower than that obtained by previous HPLC (2 ng/ml versus 10 ng/ml) [5].…”

“…Extraction of PPV in plasma was conducted by the reported method [5] with slight modification. Briefly, a 20 l of 5N NaOH solution and 5 l of internal standard solution (50 g/ml) were added to 1 ml of plasma sample in a glass test tube, respectively.…”

“…The urospasmolytic action of drug is due to the inhibition of calcium influx into smooth muscle cells and competitive antagonism with acetylcholine at muscarinic receptor sites [2]. Thin-layer chromatography (TLC) [3], gas chromatography (GC)-mass spectrometry (MS) [4], high-performance liquid chromatography (HPLC) [5] and LC-tandem mass spectrometry (MS/MS) methods [6][7][8] have been reported for the quantification of PPV in biological fluids. Among these methods, TLC method has been limited by the lack of sensitivity and selectivity and GC-MS method is complicated because of time-consuming derivatization and extraction procedures.…”

Validation of a simple liquid chromatography–tandem mass spectrometric method for the determination of propiverine hydrochloride and its N-oxide metabolite in human plasma

“…1) is an anticholinergic drug widely used in the treatment of overactive bladder symptoms. A gas chromatography-mass spectrometry (GC-MS) method [1] and a high-performance liquid chromatographic (HPLC) method [2] have been developed for the simultaneous determination of propiverine hydrochloride and its metabolites. However, the GC-MS method includes complicated pretreatments to determine numerous metabolites.…”