High-performance Liquid Chromatographic Determination of Plasma Propylthiouracil

Propylthiouracil quantification in human plasma by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry: Application in a bioequivalence study

“…Our method has good sensitivity (LOQ of 20 ng/mL) for pharmacokinetic studies, with low plasma volume (0.1 mL), without derivatization, involving a very simple liquid-liquid extraction, and can be carried out in a short time (retention time 1.76 min and run time 2.5 min), permitting a higher throughput [3,5,[12][13][14].…”

“…Various methods have been used for propylthiouracil quantification: HPLC-UV (LOQ 1.2 ng/mL, retention time 2.3 min, liquid-liquid extraction) in pharmaceutical formulations [2,10], HPLC-MS/MS (LOQ 0.5-2500 ng/mL, retention time 5.6-14 min, solid extraction, derivatization) in various animal matrices (urine, muscle, liver, thyroid, animal feed, feces and hair) [15,16] and HPLC-UV (LOQ 40-250 ng/mL, run time 3-8 min, liquid-liquid and solid extraction, 0.5-3 mL of matrix) in human plasma/serum [3,5,[12][13][14]. Our method has good sensitivity (LOQ of 20 ng/mL) for pharmacokinetic studies, with low plasma volume (0.1 mL), without derivatization, involving a very simple liquid-liquid extraction, and can be carried out in a short time (retention time 1.76 min and run time 2.5 min), permitting a higher throughput [3,5,[12][13][14].…”

“…Bioavailability and serum protein binding were between 53-88% (average 77%) and 80-85%, respectively [7]. Several analytical methods based on HPLC coupled with ultraviolet [1,5,[8][9][10][11][12][13][14], chemiluminescence [9], GC-MS [11] and LC-ESI-MS/MS after derivatization with 3-iodobenzylbromide [15,16] have been used for propylthiouracil quantification. Here, we describe a novel, fast, sensitive and specific method for quantification of propylthiouracil in human plasma using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), using methylthiouracil as an internal standard (I.S.).…”

High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine–azide reaction

“…GC-MS [5,6], HPLC-MS [4,18], HPLC with ultraviolet [7,11,13,17,18], electrochemical [18] and post-column chemiluminescence [15] detection, and TLC [20,21]. Additional requirements needed for successful results comprise the selective isolation of PTU and preconcentration steps, such as extraction including liquid-liquid extraction [10][11][12][13]15,16,18,19] solid-phase dispersion [5] or clean-up procedure with derivatization step [4,6].…”

“…The detection and determination of PTU in urine [4][5][6], plasma [7][8][9][10][11][12][13], serum [13][14][15][16], milk [5][6][7][8][9][10][11][12][13][14][15][16][17] and tissues [16,18,19] have already been investigated with the application of a variety of separation methods, e.g. GC-MS [5,6], HPLC-MS [4,18], HPLC with ultraviolet [7,11,13,17,18], electrochemical [18] and post-column chemiluminescence [15] detection, and TLC [20,21].…”