High-performance liquid chromatographic determination of acetaminophen in plasma: single-dose pharmacokinetic studies

Ameer, Barbara; Greenblatt, David J.; Divoll, Marcia; Abernethy, Darrell R.; Shargel, Leon

doi:10.1016/s0378-4347(00)84226-7

Cited by 92 publications

(24 citation statements)

References 19 publications

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“…The assay of acetaminophen was based on the methods described by Ameer et al 9) with minor modifications. To 200 ml of plasma was added 200 ml of water, 100 ml of 2-acetamidophenol solution (100 mg/mg in 20% methanol) as internal standard, and 7 ml of ethyl acetate.…”

Section: Measurement Of In Vitro Drug Releasementioning

confidence: 99%

The Influence of Gastric Acidity and Taste Masking Agent on In Situ Gelling Pectin Formulations for Oral Sustained Delivery of Acetaminophen

Itoh

Kubo

Fujiwara

et al. 2006

Biological & Pharmaceutical Bulletin

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Section: Measurement Of In Vitro Drug Releasementioning

confidence: 99%

The Influence of Gastric Acidity and Taste Masking Agent on In Situ Gelling Pectin Formulations for Oral Sustained Delivery of Acetaminophen

Itoh

Kubo

Fujiwara

et al. 2006

Biological & Pharmaceutical Bulletin

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“…The assay of acetaminophen was based on the methods described by Ameer et al, 8) with minor modifications. To 100 ml of plasma was added 200 ml of water, 100 ml of 2-acetamidophenol (100 mg/ml in 20% methanol) as internal standard, and 7 ml of ethyl acetate.…”

Section: Determination Of Acetaminophen Concentration In Rat Plasmamentioning

confidence: 99%

Carrageenan Gels for Oral Sustained Delivery of Acetaminophen to Dysphagic Patients

Miyazaki

Ishitani

Takahashi

et al. 2011

Biological & Pharmaceutical Bulletin

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“…The existing methods for the determination of paracetamol in biological fluids (blood, plasma, urine) mainly use HPLC techniques [3][4][5][6], while there are some spectrophotometric methods in which paracetamol and its conjugates present in the urine are hydrolysed with acid to paminophenol which is coupled directly with phenol in the presence of hypobromite to form an indophenol dye [7] or with vanillin to form a yellow compound with the characteristic absorbance at 395 nm [8]. Damiani et al [9] proposed the first derivative spectrophotometric method for the determination of paracetamol in blood serum.…”

Section: Introductionmentioning

confidence: 99%

Development of the second‐order derivative UV spectrophotometric method for direct determination of paracetamol in urine intended for biopharmaceutical characterisation of drug products

Parojčić

Karljiković‐Rajić

Đurić

et al. 2003

Biopharm & Drug Disp

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Paracetamol is a widely used nonsalicylate analgesic and antipyretic drug. The existing methods for the determination of paracetamol in biological fluids are mainly HPLC techniques, although there are some reported methods based on spectrophotometric determinations. However, all these methods involve some extraction or derivatisation procedures. In the present study the UV spectra of investigated samples were recorded over the wavelength range 220-400 nm (l step 0.21 nm; scan speed 60 nm/min) and second-order derivative spectra were calculated. Second-order derivative spectra of different blank urine samples displayed the presence of a zero-crossing point at 245-247 nm defined as l zc . The zero-order absorption spectra of paracetamol in water displays maximum absorbance at 243 nm, while in second derivative spectra, a minimum peak at 246 nm was observed. Therefore, the application of zero-crossing technique to the second-derivative UV absorption spectrum should be useful for the determination of paracetamol using 2 D lzc . The proposed method enables determination of total paracetamol in urine directly and simply by reading the 2 D lzc of the diluted samples. The obtained results were in good accordance with published data on cumulative urinary excretion after per oral administration of paracetamol obtained applying different spectrophotometric methods of determination. It could be useful for biopharmaceutical characterisation of drug products (monitoring of the levels of paracetamol in urine in bioavailability testing, for the evaluation of in vitro-in vivo correlation and screening of different formulations during drug product development).

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High-performance liquid chromatographic determination of acetaminophen in plasma: single-dose pharmacokinetic studies

Cited by 92 publications

References 19 publications

The Influence of Gastric Acidity and Taste Masking Agent on In Situ Gelling Pectin Formulations for Oral Sustained Delivery of Acetaminophen

The Influence of Gastric Acidity and Taste Masking Agent on In Situ Gelling Pectin Formulations for Oral Sustained Delivery of Acetaminophen

Carrageenan Gels for Oral Sustained Delivery of Acetaminophen to Dysphagic Patients

Development of the second‐order derivative UV spectrophotometric method for direct determination of paracetamol in urine intended for biopharmaceutical characterisation of drug products

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