High-performance liquid chromatographic determination of neutraceuticals, glucosamine sulphate and chitosan, in raw materials and dosage forms

El-Saharty, Yasser S.; Bary, Amany Abdel

doi:10.1016/s0003-2670(02)00279-9

Cited by 56 publications

(45 citation statements)

References 16 publications

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“…Sample hydrolysis and glucosamine derivatization were carried out following reported methods (14,15). During sample hydrolysis, protein samples of 60.0 mg, and an HCl solution (7.5 M) of 4.0 mL and a phenol solution (10.0 g/L) of 1.0 mL were successively added to glass ampoules, which were sealed by a blast burner, and heated at an electric-heated thermostatic drying oven (101-S-BS; Shanghai Hengyue Medical Instruments Co., Ltd., Shanghai, China) at 100 o C for 3 h. After ampoule breakage, the hydrolysates (1.0) mL in the ampoules were separated and neutralized to pH 7.…”

Section: Methodsmentioning

confidence: 99%

Property modification of caseinate responsible to transglutaminase-induced glycosylation and crosslinking in the presence of a degraded chitosan

Zhu

Wang

Zhao

2015

Food Sci Biotechnol

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Transglutaminase at a concentration of 10 kU/kg of protein and degraded chitosan were used for glycosylation and crosslinking of caseinate at a fixed molar ratio of the acyl acceptor to the acyl donor of 3:1, a protein concentration of 50 g/L, a pH 7.5 at 37 o C, and a reaction time of 4 h. Electrophoretic and chemical analyses showed glycosylation and crosslinking of caseinate. Glycosylated and crosslinked caseinate (GC-caseinate) contained glucosamine at 12.77 g/kg of protein, and the protein fraction had fewer reactable amino groups than original caseinate (0.58 vs. 0.64 mol/kg of protein). GC-caseinate exhibited an enhanced surface hydrophobicity, in vitro digestibility, water-binding capacity, and rheological properties, with poor protein dispensability and emulsification activity, but a similar oilbinding capacity and emulsion stability, compared with original caseinate. GC-caseinate also exhibited better properties than transglutaminase-crosslinked caseinate. Glycosylation and crosslinking was effective for better water-binding and rheological properties of caseinate.

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Section: Methodsmentioning

confidence: 99%

Property modification of caseinate responsible to transglutaminase-induced glycosylation and crosslinking in the presence of a degraded chitosan

Zhu

Wang

Zhao

2015

Food Sci Biotechnol

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“…Glucosamine in pharmaceutical formulations, nutraceuticals, and raw bulk materials is determined by HPLC with RI detection (El-Saharty & Bary 2002), evaporative light scattering detection (Jacyno & Thrall 2004), direct UV detection at 195 nm (Shao et al 2004), and UV detection at 254 nm after derivatisation with phenylisothiocyanate (Liang et al 1999). Besides HPLC technique, capillary electrophoresis (CE) with UV detection (214 nm) after the derivatisation of GA with anthranilic acid (Qi et al 2006) or with conductometric detection (Jáč et al 2008) without derivatisation have been used.…”

mentioning

confidence: 99%

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Václavíková¹,

Kvasnička²

2013

Czech J. Food Sci.

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Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech J. Food Sci., 31: 55-65.Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many of the marketed products. We present here a capillary isotachophoretic method for the determination of glucosamine and chondroitin sulfate in dietary supplements. Cationic analysis of glucosamine was performed with a leading electrolyte consisting of 10mM NH 4 OH + 20mM acetic acid. As the leading electrolyte for anionic analysis of chondroitin sulphate, a mixture of 5mM HCl + 10mM glycylglycine + 0.05% of 2-hydroxyethylcellulose was used. The solution of 10mM citric acid served as the terminating electrolyte for both glucosamine and chondroitin sulfate analyses. The analytes were detected by conductivity and UV detectors. The characteristics of the method,, i.e., linearity, accuracy, repeatability, and quantitation limit, were evaluated. On a set of 35 samples of marketed dietary supplements did we prove that the capillary isotachophoresis is a suitable method for the routine analysis of glucosamine and chondroitin sulfate.

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“…Therefore, refractive index detectors have been employed for the HPLC analysis of glucosamine. 7,8 In addition to the detection difficulty, a relatively cumbersome normal phase LC separation is required for the direct analysis of glucosamine in pharmaceutical products. 2,[5][6][7][8] Pre-column derivatization has been employed to overcome the detection difficulty and/or to adopt a reverse phase LC separation.…”

Section: Introductionmentioning

confidence: 99%

“…7,8 In addition to the detection difficulty, a relatively cumbersome normal phase LC separation is required for the direct analysis of glucosamine in pharmaceutical products. 2,[5][6][7][8] Pre-column derivatization has been employed to overcome the detection difficulty and/or to adopt a reverse phase LC separation. [9][10][11] Recently, a normal phase LC separation with ESI/MS detection method has been employed for the direct determination of glucosamine in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Development of an Isotope-Dilution Flow-Injection Electrospray/ Mass Spectrometric Method for the Accurate Determination of Glucosamine in Pharmaceutical Formulation

Kim¹,

Kim²,

Ahn³

et al. 2009

Bulletin of the Korean Chemical Society

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An isotope-dilution flow-injection electrospray/mass spectrometric method was developed for the accurate determination of glucosamine contents in pharmaceutical formulations. Samples were extracted by methanol. After spiking glucosamine-1-13 C1 as an internal standard, the extracts were then analyzed by flow-injection ESI/MS in a selected ion monitoring (SIM) mode to detect [M+H] + ions of the analyte and its isotope analogue at m/z 180 and m/z 181, respectively. Confirmatory measurements were made by selectively monitoring the collisionally induced dissociation channels of m/z 180 → m/z 72 and m/z 181 →73, respectively, to test the possibility of bias in the SIM method due to matrix interferences, but any significant bias in the SIM mode was not observed. Repeatability and reproducibility studies showed that the flow-injection ESI/MS method is a reliable and reproducible method which can provide a typical method precision of 1.0 %. Other results for the method validation are reported.

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High-performance liquid chromatographic determination of neutraceuticals, glucosamine sulphate and chitosan, in raw materials and dosage forms

Cited by 56 publications

References 16 publications

Property modification of caseinate responsible to transglutaminase-induced glycosylation and crosslinking in the presence of a degraded chitosan

Property modification of caseinate responsible to transglutaminase-induced glycosylation and crosslinking in the presence of a degraded chitosan

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Development of an Isotope-Dilution Flow-Injection Electrospray/ Mass Spectrometric Method for the Accurate Determination of Glucosamine in Pharmaceutical Formulation

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