High-performance liquid chromatographic method for the determination of HIV-1 non-nucleoside reverse transcriptase inhibitor efavirenz in plasma of patients during highly active antiretroviral therapy

A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

“…Up to now, several methods for the determination of efavirenz in plasma using LC-MS (17,18), LC-MS-MS (19,20), MALDI-TOF/IOF (21), HPLC-PAD (22)(23)(24)(25), etc., have been published. These methods may not be widely used in underdeveloped countries because of the inaccessibility and high costs of laboratory equipments.…”

“…Also, many HPLC-UV methods for determination of plasma efavirenz (23,(26)(27)(28)(29)(30)(31) or simultaneous determination with other drugs such as anti-tuberculosis (anti-TB) agents or other antiretroviral agents (24,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) have been reported. In most of these methods, the volumes of plasma used range from 200 to 900 μL (23,(26)(27)(28)33,34,(44)(45)(46), which were inapplicable for children due to the scarcity of sample. High-cost, complicated and time-consuming plasma sample pre-treatments such as solid-phase extraction (26,33,34,(44)(45)(46), liquid-liquid extraction (23,24,27,28,32,…”

“…In most of these methods, the volumes of plasma used range from 200 to 900 μL (23,(26)(27)(28)33,34,(44)(45)(46), which were inapplicable for children due to the scarcity of sample. High-cost, complicated and time-consuming plasma sample pre-treatments such as solid-phase extraction (26,33,34,(44)(45)(46), liquid-liquid extraction (23,24,27,28,32,35) appeared in some methods. A few of reported methods were difficult to repeat because of the lack of internal standard (26).…”

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

“…This is because of its long half-life, which means effective concentrations are maintained for long periods. 3 Several methods are described in literature for the quantification of efavirenz in biological fluids by high performance liquid chromatography with ultraviolet detection (HPLC-UV), [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] HPLC with fluorescence detector, 23 liquid chromatography with mass spectrometry detection (LC-MS), 24 liquid chromatography with mass spectrometry in tandem detection(LC-MS/MS) [25][26][27][28][29] and matrix assisted laser desorption/ionization with time-of-flight in tandem detection (MALDI-TOF/ TOF). 30 When sample throughput is an important issue, such as in pharmacokinetic applications, the development of rugged methods with short analysis times becomes an important consideration.…”

Desenvolvimento de método analítico para quantificação do efavirenz por espectrofotometria no UV-Vis

“…2 Na literatura científica internacional, diversos métodos analí-ticos para a quantificação do EFZ são preconizados utilizando-se cromatografia líquida de alta eficiência (CLAE) do fármaco isolado e na forma farmacêutica cápsula, 3 assim como em plasma na forma isolada e associado a outros antirretrovirais. [4][5][6][7][8][9][10][11][12][13][14] Contudo, nenhum método analítico por espectrofotometria de absorção no ultravioleta visível (UV-vis) foi relatado.…”