High Performance Liquid Chromatographic Method for the Determination of Sodium Alginate in Foods

Toyoda, Masatake; Yomota, Chikako; Ito, Yoshio; Harada, Motoo

doi:10.3358/shokueishi.26.189

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Assays Based On Degradation Of Uronic Acids1

Assay For Alginic Acid1

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1999

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Cited by 6 publications

(2 citation statements)

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“…The reaction with Fe 2 (SO 4 ) 3 was successfully used for the study of seasonal changes and anatomic distribution of alginates in some species of brown algae. 106 In a more complicated procedure described by Toyoda et al, 107 naphthoresorcinol-HCl was added to an alginate after its precipitation as Cu 2+ salt; the coloured reaction product formed thereupon was extracted with butyl acetate and quantitatively assayed using HPLC.…”

Section: Assays Based On Degradation Of Uronic Acidsmentioning

confidence: 99%

Alginic acids and alginates: analytical methods used for their estimation and characterisation of composition and primary structure

Усов¹

1999

Russ. Chem. Rev.

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Section: Assays Based On Degradation Of Uronic Acidsmentioning

confidence: 99%

Alginic acids and alginates: analytical methods used for their estimation and characterisation of composition and primary structure

Усов¹

1999

Russ. Chem. Rev.

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“…Alginic acid production was quantified by high-performance liquid chromatogra phy (HPLC; C-R4A, Shimadzu. Kyoto, Japan) accord ing to the method ofToyoda et al [11], HPLC A 25-cm, 4.6-mm-internal-diamcter Finepak SILCig column (Japan Spectroscopic, Tokyo. Japan) was used with acetonitrile-(Sigma, Tokyo, Japan)water-butyl acetate (75:20:5) as the mobile phase.…”

Section: Assay For Alginic Acidmentioning

confidence: 99%

The Influence of Azithromycin on the Biofilm Formation of <i>Pseudomonas aeruginosa</i> in vitro

et al. 1996

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The influence of azithromycin on biofilm formation by Pseudomonas aeruginosa, a cause of refractory chronic respiratory tract infection, was investigated. Alginic acid produced by a mucoid strain of P. aeruginosa was quantified by high-performance liquid chromatography from colonies growing on an agar medium. Polysaccharides in the biofilm formed on silicon chips by a nonmucoid strain were determined by a tryptophan reaction. The effect of azithromycin was examined at concentrations below the minimum inhibitory concentration (sub-MIC) for each strain. Azithromycin significantly inhibited the production of alginic acid from the mucoid strain at ≥ 1/256 MIC, and the production of exopolysaccharides from the nonmucoid strain at ≥ 1/16 MIC. The inhibition of biofilm formation by azithromycin was also observed by scanning electron microscopy. These findings suggest that azithromycin inhibits biofilm formation by P. aeruginosa at concentrations well below the MIC.

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[43] In vitro and in Vivo models of bacterial biofilms

Yasuda¹,

Koga²,

Fukuoka³

1999

Biofilms

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High Performance Liquid Chromatographic Method for the Determination of Sodium Alginate in Foods

Cited by 6 publications

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Alginic acids and alginates: analytical methods used for their estimation and characterisation of composition and primary structure

Alginic acids and alginates: analytical methods used for their estimation and characterisation of composition and primary structure

The Influence of Azithromycin on the Biofilm Formation of <i>Pseudomonas aeruginosa</i> in vitro

[43] In vitro and in Vivo models of bacterial biofilms

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