High-performance liquid chromatographic preparation of galactosyl-hydroxylsine, a specific bone collagen marker

Moro, Luigi; Battista, Cinzia; Modricky, C.; Rovis, L; Bernard, B. de; Traldi, Pietro; Lubrano, Giovanni; Marotti, Francesco

doi:10.1016/s0378-4347(00)82786-3

Cited by 14 publications

(7 citation statements)

References 7 publications

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“…The posttranslational modification of hydroxylysine by glycosylation with ga lactose is specific for bone collagen and after bone resorp tion by osteoclasts GHY is finally excreted in the urine [17], The excretion pattern of GHY under therapy with GH is comparable to OHP excretion.…”

Section: Discussionmentioning

confidence: 99%

“…Procollagen peptides in serum were measured by RIA using test kits (RIA-gnost PIUP, Behring, Marburg, Germa ny, and Procollagen PICP RIA kit, Farmos, Turku, Finland). Galactosylhydroxylysine (GHY) excretion in urine was measured by highperformance liquid chromatography according to Moro et al [17], Values are calculated as a ratio of GHY to creatinine excretion in urine.…”

Section: Assaysmentioning

confidence: 99%

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Dose-Dependent Responses in Insulin-Like Growth Factors, Insulin-Like Growth Factor-Binding Protein-3 and Parameters of Bone Metabolism to Growth Hormone Therapy in Young Adults with Growth Hormone Deficiency

Wollmann

Schönaü²,

Blum

et al. 1995

Horm Res

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There is increasing awareness that growth hormone (GH) replacement therapy is also essential in adult patients with growth hormone deficiency (GHD). There are little data available on the dose requirements for replacement therapy in this age group. In childhood, the growth response to GH therapy can serve as an indicator for the correct replacement dose. Because this indicator does not exist in adults, we analyzed growth factors and biochemical markers of bone metabolism by specific radioimmunoassays in a group (n = 12) of adult patients (age, 20.0-31.6 years) with GHD with childhood onset before and after a 4-week treatment period (daily, s.c.) with recombinant, human GH at different doses (0.125, 0.25 and 0.5 IU/kg body weight/week). Comparing the basal levels to those on low-dose GH (0.125 IU/kg/week) and on a high dose (0.5 IU/kg/week), the following results were obtained. Insulin-like growth factor-I (IGF-1) in serum: basal, 68.6 ± 37 ng/ml; low dose, 176.9 ± 65 ng/ml (p ≤ 0.05); high dose, 380.6 ± 200 ng/ml (p < 0.01). IGF-binding protein-3 in serum: basal, 2.13 ± 0.58 mg/l; low dose, 3.23 ± 0.84 mg/l (p < 0.01); high dose, 3.97 ± 0.82 mg/l. Osteocalcin in serum: basal, 3.88 ± 1.27 ng/ml; low dose, 7.01 ± 2.20 ng/ml (p < 0.01); no further increase. Procollagen-I peptide in serum: basal, 113.6 ± 36.7 μg/l; low dose, 211.6 ± 90.4 μg/l (p < 0.01); no further increase. Galactosylhydroxylysine excretion in urine: basal, 12.9 ± 5.24 μmol/g; low dose, 23.3 ± 13.2μmol/g(p < 0.05); high dose, 27.7 ± 13.7 μmol/g. We conclude that the parameters measured possibly represent a useful tool for the assessment of the correct replacement dose in adult GHD. As the lowest dose investigated (0.125 IU/kg body weight/week) normalized serum levels of growth factors and parameters of bone metabolism, this dose might be the advisable replacement dose in adult GHD.

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Section: Discussionmentioning

confidence: 99%

Section: Assaysmentioning

confidence: 99%

Dose-Dependent Responses in Insulin-Like Growth Factors, Insulin-Like Growth Factor-Binding Protein-3 and Parameters of Bone Metabolism to Growth Hormone Therapy in Young Adults with Growth Hormone Deficiency

Wollmann

Schönaü²,

Blum

et al. 1995

Horm Res

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“…The area of the peaks was calculated by integration. The values of hydroxylysine and hydroxylysine glycosides were calculated using L-lysine as an external standard as previously described [15].…”

Section: Measurementsmentioning

confidence: 99%

Posttranslational Modifications of Bone Collagen Type I are Related to the Function of Rat Femoral Regions

Moro

Romanello

Favia

et al. 2000

Calcified Tissue International

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This study analyzes the relationship between the function of femoral regions in the rat and the extent of collagen type I posttranslational modifications, to assess whether the different functional roles, i.e., mechanical or metabolic, of the bone tissues are related to the molecular structure of the matrix. For this purpose, 18 female, 100-day-old Sprague-Dawley rats were sacrificed, under anesthesia, and their femurs were removed and dissected free of adhering tissue. The spongy bone of the proximal metaphysis and the diaphysis were then selected as regions exerting prevalently a mechanical function, and the spongy bone of the distal metaphysis was selected as mainly related to metabolic function. Bone prepared from these regions was used to extract and purify the major component of the matrix, type I collagen. The content of hydroxyproline, hydroxylysine, glycosylated hydroxylysine, and pyridinium crosslinks was evaluated and the amount of each compound was expressed as a molar ratio to hydroxyproline. The amount of glycosylated hydroxylysine and pyridinium crosslinks in the distal metaphysis are significantly different from the amounts measured both in the diaphysis and the proximal metaphysis. On the contrary, the amounts of the same compounds in the diaphysis and the proximal metaphysis are statistically the same. The amount of free hydroxylysine, however, appears to be different in the proximal metaphysis and in the diaphysis. The conclusion is that matrix composition differs among different skeletal regions according to the main function they exert.

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“…GHyl has a potential use as a marker of bone resorption, but it has never been a subject of intense development because of the analytical complexity of the original assay (6). The newest assays are simpler, more practical (7)(8)(9), and allow a reconsideration of GHyl as a candidate marker of bone resorption.…”

Section: Introductionmentioning

confidence: 99%

Responsiveness of urinary markers of bone resorption to orchiectomy and clodronate treatment in mature rats: a comparative study

Rubinacci¹,

Villa

Sibilia³

et al. 1998

European Journal of Endocrinology

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Objective: The aim of this study was to assess the responsiveness of two classes of bone resorption markers to the enhancement of osteoclastic activity induced by orchiectomy and to its inhibition by clodronate treatment in mature rats. Design: Bone mineral density (BMD) at femural metaphysis, femural diaphysis, lumbar vertebrae, and the urinary excretion of pyridinoline (Pyr), deoxypyridinoline (D-Pyr), galactosylhydroxylysine (GHyl) and glucosylgalactosylhydroxylysine (GGHyl) were monitored at regular intervals for 30 days prior to and for 60 days following orchiectomy in eleven rats, divided into two groups: five rats untreated and the other six treated with clodronate. Results: Prior to orchiectomy, a significant (P < 0.01) decrease in BMD was observed only at the distal femural metaphysis. This decrease appeared to be associated with a time-dependent increase in the urinary excretion of all markers. Following orchiectomy, the BMD of the untreated group decreased significantly (P < 0.01) at all bone sites. The bone loss was accompanied by a significant (P < 0.01) increase in Pyr and D-Pyr concentrations in urine, whereas urinary GHyl and GGHyl did not change significantly. In the clodronate-treated group, the BMD of the three skeletal sites did not change significantly, while the urinary excretion of all urinary biochemical markers decreased significantly (P < 0.001). Conclusion: This study showed that pyridinolines are able to monitor the bone response to orchiectomy and to clodronate treatment response in androgen-deficient mature male rat, whereas glycosides appear prone to confounding factors.

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High-performance liquid chromatographic preparation of galactosyl-hydroxylsine, a specific bone collagen marker

Cited by 14 publications

References 7 publications

Dose-Dependent Responses in Insulin-Like Growth Factors, Insulin-Like Growth Factor-Binding Protein-3 and Parameters of Bone Metabolism to Growth Hormone Therapy in Young Adults with Growth Hormone Deficiency

Dose-Dependent Responses in Insulin-Like Growth Factors, Insulin-Like Growth Factor-Binding Protein-3 and Parameters of Bone Metabolism to Growth Hormone Therapy in Young Adults with Growth Hormone Deficiency

Posttranslational Modifications of Bone Collagen Type I are Related to the Function of Rat Femoral Regions

Responsiveness of urinary markers of bone resorption to orchiectomy and clodronate treatment in mature rats: a comparative study

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