High-Performance Liquid Chromatography Determination of Praziquantel in Rat Plasma; Application to Pharmacokinetic Studies

Abstract

A very simplistic, selective, sensitive, and reproducible procedure based on a reversed-phase highperformance liquid chromatography was used and developed for the determination of praziquantel in rat plasma. For the separation of praziquantel from the internal standard, diazepam on an Enable, C18 column (250×4.6 mm, 5 µm particle size), with the retention time of 6.4, 8.5 min, respectively. For praziquantel with UV detector at 225 nm. The mobile phase was a mixture of acetonitrile:water in a ratio of (60:40 v/v), running through the column at the flow rate of 1 ml/min. Sample preparation of 200 µl of plasma was done by a protein precipitation by using perchloric acid. Calibration curve was found to be linear with correlation coefficients (r 2 ) is 0.9989 prepared in plasma at the concentrations of 5 to 1000 ng/ml. The precision of the above method was based on inter-day, and intra-day repeatability, and reproducibility (day-to-day variation) were found to be within the limit of 15%. Limit of quantification was accepted and found to be 5 ng/ml using 200 µl samples. The method appears to be robust and has been applied to a pharmacokinetic study of praziquantel in three groups of rats with a single oral dose of 40 mg/kg body weight.

Clinical significance of the expression of miRNA-21, miRNA-31 and miRNA-let7 in patients with lung cancer

“…The ROC curve showed that the three miRNA had clinical diagnosis efficiency for lung cancer, and the combined detection of the three miRNAs were more efficient in diagnosing lung cancer. Survival curve analysis suggested that the median survival times of patients in the miRNA-21 and miRNA-31 high expression groups were shorter than those in the low expression groups, and the median survival time of patients in miRNA-let7 high expression group was longer than that in the low expression group, indicating that the expression level of miRNA may be related to disease progression (Dey et al, 2017, Gao et al, 2017, Ge et al, 2018, Peng et al, 2017, Bokhari et al, 2018, Yildirim et al, 2017).…”

Bioanalytical method development and validation of folic acid from rat plasma using reverse phase high performance liquid chromatography

“…After the recovery the samples were dried and reconstituted in respective 300 µl of selected mobile phase. Mobile phase was filtered through 0.45 µm syringe filter and analyzed for folic acid concentration by HPLC [19,20].…”

A mathematical model for the control of swimmer's itch

“…Since exposed and infected birds cannot be identified based on phenotype, we assume that the number of exposed and infected birds that are transitioned back into the susceptible population is times the prevalence within each infected stage of the bird population at time . Research completed on a number of different vertebrates has shown that praziquantel efficacy can be limited due to its elimination from the body within days (Arion, Fernández‐Varón, Cárceles, Gagyi, & Ognean, 2018; Chai, 2013; Dey et al, 2018). Consequently, we assume that birds become susceptible immediately after praziquantel treatment.…”