A very simplistic, selective, sensitive, and reproducible procedure based on a reversed-phase highperformance liquid chromatography was used and developed for the determination of praziquantel in rat plasma. For the separation of praziquantel from the internal standard, diazepam on an Enable, C18 column (250×4.6 mm, 5 µm particle size), with the retention time of 6.4, 8.5 min, respectively. For praziquantel with UV detector at 225 nm. The mobile phase was a mixture of acetonitrile:water in a ratio of (60:40 v/v), running through the column at the flow rate of 1 ml/min. Sample preparation of 200 µl of plasma was done by a protein precipitation by using perchloric acid. Calibration curve was found to be linear with correlation coefficients (r 2 ) is 0.9989 prepared in plasma at the concentrations of 5 to 1000 ng/ml. The precision of the above method was based on inter-day, and intra-day repeatability, and reproducibility (day-to-day variation) were found to be within the limit of 15%. Limit of quantification was accepted and found to be 5 ng/ml using 200 µl samples. The method appears to be robust and has been applied to a pharmacokinetic study of praziquantel in three groups of rats with a single oral dose of 40 mg/kg body weight.

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Teenagers face challenges due to the lack of information about sex, sexuality, body changes, puberty, teenage pregnancy, early childbearing, HIV/AIDS in most developing countries, and Bangladesh is no exception. However, the study aims to explore the contributing factors of inadequate sex education at schools in Bangladesh. The study reviewed a variety of secondary sources and literatures to understand the contributing factors of inadequate sex education and analyzes those data and literatures. Therefore, the study found that there is a lack of a comprehensive sex education policy and formal sex education is very limited in Bangladesh. Moreover, it also found that there are very few chapters about sex education in the biological book, but teachers feel uncomfortable teaching about these topics. Furthermore, the parents are not confident to talk with their children as they have insufficient knowledge about sex education topics. In addition, the cultural and religious barriers are also responsible for inadequate sex education due to the orthodox thoughts of the family and society. The paper recommends that there should have a comprehensive sex education policy, and curriculum. Moreover, the focus should be on the implementation of interventions for the teachers' training, parent’s session, & community engagement programs. Keywords: Adolescent, Sex education, Parent’s attitude, Teachers’ perception, Bangladesh