High-performance liquid chromatography method for the quantification of rabeprazole in human plasma using solid-phase extraction

Ramakrishna, N. V. S.; Vishwottam, K.N.; Wishu, S.; Koteshwara, M.; Kumar, S. Suresh

doi:10.1016/j.jchromb.2004.11.032

Cited by 35 publications

(23 citation statements)

References 9 publications

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“…Literature survey revealed that capillary electrophoresis method 1 , liquid chromatography-tandem mass spectrometry 2 , RP-HPLC method 3,4 , spectrophotometric and chromatographic determination 5 , spectrophotometric method [6][7][8] were reported for determination of rabeprazole in tablet dosage forms. Various methods were reported in literature for determination of rabeprazole in combination with other drugs which includes, HPLC method [9][10][11][12][13][14] , HPTLC method [15][16][17] , column reversed-phase high-performance liquid chromatographic method 18 , spectrophotometric method [19][20][21][22][23][24][25][26][27][28][29][30] .…”

Section: Rabeprazole (Ra) or 2-[[[4-(3-methoxypropoxy)-3-methyl-2-pymentioning

confidence: 99%

A Simple Spectrophotometric Determination of Rebeprazole in Pharmaceutical Preparations

M¹,

T²,

Prabhavathi³

2012

Chem Sci Trans

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A simple, sensitive spectrophotometric method was developed for the determination of rabeprazole in pure form and for pharmaceutical formulations. In this method 2,3-dichloro-5, 6-dicyano-p-benzoquinone (DDQ) was utilized for determination of rabeprazole forming charge transfer complex with maximum absorbance at λ max 460 nm. Variables affecting the reaction were studied and optimized. Beer's law was obeyed in concentration range 20-100 µg/mL for rabeprazole. The accuracy and reproducibility of the proposed method was statistically validated by recovery studies. The proposed method is simple, rapid and validated and can be used successfully for routine analysis of rabeprazole in a pure and tablet dosage form.

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Section: Rabeprazole (Ra) or 2-[[[4-(3-methoxypropoxy)-3-methyl-2-pymentioning

confidence: 99%

A Simple Spectrophotometric Determination of Rebeprazole in Pharmaceutical Preparations

M¹,

T²,

Prabhavathi³

2012

Chem Sci Trans

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“…Chaque étape de l'extraction a été améliorée individuellement [7,12,13,15]. Les phases de lavage et d'élution ont éga-lement été étudiées afin de minimiser les pertes de molécules d'intérêt et pour maximiser la récupération des molécules adsorbées sur la phase de la colonne.…”

Section: Matériel Et Méthodesunclassified

“…Pour chaque paramètre, des séries de trois essais ont été exécutées. Tous les tests ont été réali-sés sur des mélanges contenant 6 à 8 molécules choisies de manière à obtenir un tracé chromatographique (figure 1) sans phénomène de co-élution [13,14,16]. Ainsi il a été avéré l'absence d'interférence d'un résultat sur l'autre pour les calculs de rendements d'extraction.…”

Section: Matériel Et Méthodesunclassified

“…La dernière phase de nos travaux a consisté en la comparaison des limites de détection et quantification obtenues avec des valeurs de la littérature recueillies en milieu hospitalier ou en laboratoire de toxicologie médico-légale [4,6,13,[17][18][19] ; il est nécessaire de savoir si les limites obtenues pour ces molé-cules sont cohérentes avec les concentrations auxquelles elles …”

Section: Matériel Et Méthodesunclassified

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Extraction en phase solide de 22 médicaments d'intérêt en toxicologie médico-légale

Mancebo¹,

Perrin

2008

Ann Toxicol Anal

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Résumé -De nombreuses colonnes d'extraction en phase solide dédiées à l'extraction des toxiques et leurs protocoles spécifiques sont proposées à l'analyste. Nous avons optimisé l'un de ces protocoles pour l'extraction à partir de sang total -en une seule étape -de principes actifs de médicaments acides, neutres et basiques. Chaque niveau du protocole a été conçu de manière à obtenir les meilleurs rendements d'extraction. L'analyse des extraits (identification et quantification) a été réalisée à l'aide d'un système de High Performance Liquid Chromatography system with Diode Array Detector (HPLC-DAD). La colonne SPE Evolute ABN r (Acid Base Neutral) fournie par Argonaut est celle qui extrait le mieux les différents composés analysés parmi toutes les cartouches testées. La colonne Oasis HLB r (Hydrophilic Lipophilic Balance) proposée par Waters permet également l'extraction de la totalité des molécules mais avec des rendements inférieurs. Quant aux autres colonnes étudiées (distribuées par Varian et Phenomenex), elles ont pré-senté des résultats insuffisants dans le cadre de ce projet. Les limites de détection et de quantification obtenues avec ce protocole optimisé ont été comparées aux concentrations thérapeutiques et toxiques habituellement retrouvées. La mise au point de cette nouvelle procédure a permis d'optimiser une méthode de dépistage large (criblage) de médicaments fréquemment rencontrés dans les matrices biologiques en toxicologie clinique et médico-légale. Mots clés : Extraction en phase solide, colonnes SPE, médicaments, intoxication, criblageAbstract -Solid phase extraction of 22 drugs of interest in the forensic toxicology field. Many solid phase extraction columns which are dedicated to the extraction of various drugs associated with their specific protocols are proposed to the analyst. We have optimised a one stage procedure for the extraction from whole blood of acidic, neutral and basic drugs. Each step of the protocol has been optimised so as to obtain the best extraction recovery. The analysis of the extracts (identification and quantification) has been realised with the help of High Performance Liquid Chromatography system coupled with a Diode Array Detector (HPLC-DAD). The SPE Evolute ABN r column (Acid Base Neutral) by Argonaut is the column which does extract the more and the best the different analysed components among all the columns which have been tested. Oasis HLB r column (Hydrophilic Lipophilic Balance) by Waters also allows the extraction of the whole list of active principles studied but with lower recoveries. The other columns studied (sold by Varian and Phenomenex) presented insufficient results for this project. In the last step of the work we checked if the results did fit to the forensic toxicology purpose; detection and quantification limits have been compared to therapeutic and toxic concentrations usually found in forensic toxicology. Implementing this new procedure allowed us to create a method of comprehensive screening of drugs frequently implicated in forensic toxicolo...

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“…Aceclofenac directly blocks prostaglandin (PGE2) secretion at the site of inflammation by inhibiting interleukin (IL1) and tumour necrosis factor (TNF) in the inflammatory cells (intracellular action) . Literature review reveals that Spectrophotometric (Kousy et al, 1999;Zawilla et al,2002;Rahman et al, 2008;ElGindy et al, 2003;Shah et al, 2008;Mishra et al, 2006) HPLC (Singhvi et al, 2007;Park et al, 2008;Ramakrishna et al, 2005;Srinivas et al, 2009;Uno et al, 2005;Singh et al, 2004;Rao et al, 2006;Kulkarni et al, 2006;Jin et al, 2006;Bhinge et al, 2008;Hinz et al, 2006;Musmade et al, 2008) and HPTLC (Joshi et al, 2008) methods have been reported for estimation of individual drugs or in combination with other drugs in formulation. So far no simultaneous method has been reported as this was a new combined dosage form.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC analysis for the simultaneous estimation of rabeprazole sodium and aceclofenac in a combined dosage form

2012

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A rapid, simple and highly sensitive reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative determination of Rabeprazole sodium and Aceclofenac in a combined dosage form. Rabeprazole sodium and Aceclofenac were chromatographed using C-18 column as stationary phase and methanol: acetonitrile: water (60 : 10 : 30 v/v/v) as the mobile phase at a flow rate of 1.0 ml/min at ambient temperature and detected at 280 nm. The retention time (RT) of Rabeprazole sodium and Aceclofenac were found to be 5.611 min and 2.102 minute, respectively. The linearities of Rabeprazole sodium and Aceclofenac were in the range of 1-10 µg/ml and 3-15 µg/ml, respectively. The limit of detection was found to be 0.091 µg/ml for Rabeprazole sodium and 0.043 µg/ml for Aceclofenac. The proposed method was applied for the determination of Rabeprazole sodium and Aceclofenac in a combined dosage form and result was found satisfactory.DOI: http://dx.doi.org/10.3329/icpj.v1i12.12450 International Current Pharmaceutical Journal 2012, 1(12): 410-413

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High-performance liquid chromatography method for the quantification of rabeprazole in human plasma using solid-phase extraction

Cited by 35 publications

References 9 publications

A Simple Spectrophotometric Determination of Rebeprazole in Pharmaceutical Preparations

A Simple Spectrophotometric Determination of Rebeprazole in Pharmaceutical Preparations

Extraction en phase solide de 22 médicaments d'intérêt en toxicologie médico-légale

RP-HPLC analysis for the simultaneous estimation of rabeprazole sodium and aceclofenac in a combined dosage form

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